Stage-dependent requirement of neuroretinal Pax6 for lens and retina development
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24523460
DOI
10.1242/dev.098822
PII: dev.098822
Knihovny.cz E-resources
- Keywords
- Lens induction, Pax6, Retinal progenitor, mRx-Cre,
- MeSH
- Cell Differentiation genetics physiology MeSH
- Embryonic Stem Cells cytology metabolism MeSH
- Gene Knockdown Techniques MeSH
- Homeodomain Proteins antagonists & inhibitors genetics metabolism MeSH
- Cell Cycle Checkpoints genetics physiology MeSH
- Mice, Knockout MeSH
- Mice, Transgenic MeSH
- Mice MeSH
- Neural Stem Cells cytology metabolism MeSH
- Lens, Crystalline embryology metabolism MeSH
- Eye Proteins antagonists & inhibitors genetics metabolism MeSH
- Repressor Proteins antagonists & inhibitors genetics metabolism MeSH
- Retina cytology embryology metabolism MeSH
- Pregnancy MeSH
- Trans-Activators genetics metabolism MeSH
- PAX6 Transcription Factor MeSH
- Paired Box Transcription Factors antagonists & inhibitors genetics metabolism MeSH
- Gene Expression Regulation, Developmental MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- cone rod homeobox protein MeSH Browser
- Homeodomain Proteins MeSH
- Eye Proteins MeSH
- Pax6 protein, mouse MeSH Browser
- Repressor Proteins MeSH
- Trans-Activators MeSH
- PAX6 Transcription Factor MeSH
- Paired Box Transcription Factors MeSH
The physical contact of optic vesicle with head surface ectoderm is an initial event triggering eye morphogenesis. This interaction leads to lens specification followed by coordinated invagination of the lens placode and optic vesicle, resulting in formation of the lens, retina and retinal pigmented epithelium. Although the role of Pax6 in early lens development has been well documented, its role in optic vesicle neuroepithelium and early retinal progenitors is poorly understood. Here we show that conditional inactivation of Pax6 at distinct time points of mouse neuroretina development has a different impact on early eye morphogenesis. When Pax6 is eliminated in the retina at E10.5 using an mRx-Cre transgene, after a sufficient contact between the optic vesicle and surface ectoderm has occurred, the lens develops normally but the pool of retinal progenitor cells gradually fails to expand. Furthermore, a normal differentiation program is not initiated, leading to almost complete disappearance of the retina after birth. By contrast, when Pax6 was inactivated at the onset of contact between the optic vesicle and surface ectoderm in Pax6(Sey/flox) embryos, expression of lens-specific genes was not initiated and neither the lens nor the retina formed. Our data show that Pax6 in the optic vesicle is important not only for proper retina development, but also for lens formation in a non-cell-autonomous manner.
References provided by Crossref.org
Ancestral role of Pax6 in chordate brain regionalization
Dose-dependent regulation of horizontal cell fate by Onecut family of transcription factors
Genetic interaction between Pax6 and β-catenin in the developing retinal pigment epithelium
