Comparative analysis of respiratory chain and oxidative phosphorylation in Leishmania tarentolae, Crithidia fasciculata, Phytomonas serpens and procyclic stage of Trypanosoma brucei
Language English Country Netherlands Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
24556248
DOI
10.1016/j.molbiopara.2014.02.003
PII: S0166-6851(14)00019-X
Knihovny.cz E-resources
- Keywords
- Crithidia, Leishmania, Mitochondrion, Oxidative phosphorylation, Phytomonas, Trypanosoma,
- MeSH
- Glycerolphosphate Dehydrogenase metabolism MeSH
- Oxygen metabolism MeSH
- Membrane Potential, Mitochondrial MeSH
- Mitochondria enzymology metabolism MeSH
- Oxidative Phosphorylation * MeSH
- Electron Transport MeSH
- Trypanosomatina genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Glycerolphosphate Dehydrogenase MeSH
- Oxygen MeSH
Trypanosomatids are unicellular parasites living in a wide range of host environments, which to large extent shaped their mitochondrial energy metabolism, resulting in quite large differences even among closely related flagellates. In a comparative manner, we analyzed the activities and composition of mitochondrial respiratory complexes in four species (Leishmania tarentolae, Crithidia fasciculata, Phytomonas serpens and Trypanosoma brucei), which represent the main model trypanosomatids. Moreover, we measured the activity of mitochondrial glycerol-3-phosphate dehydrogenase, the overall oxygen consumption and the mitochondrial membrane potential in each species. The comparative analysis suggests an inverse relationship between the activities of respiratory complexes I and II, as well as the overall activity of the canonical complexes and glycerol-3-phosphate dehydrogenase. Our comparative analysis shows that mitochondrial functions are highly variable in these versatile parasites.
Biology Centre Institute of Parasitology České Budějovice Czech Republic
Department of Biochemistry Faculty of Natural Sciences Comenius University Bratislava Slovakia
References provided by Crossref.org
Distribution and Functional Analysis of Isocitrate Dehydrogenases across Kinetoplastids
Kinetoplast Genome of Leishmania spp. Is under Strong Purifying Selection
Highly flexible metabolism of the marine euglenozoan protist Diplonema papillatum
Differences in mitochondrial NADH dehydrogenase activities in trypanosomatids
ZapE/Afg1 interacts with Oxa1 and its depletion causes a multifaceted phenotype
Molecular mechanisms of thermal resistance of the insect trypanosomatid Crithidia thermophila
