Gold nanoparticle-based immuno-PCR for detection of tau protein in cerebrospinal fluid
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24642424
DOI
10.1016/j.jim.2014.03.007
PII: S0022-1759(14)00081-7
Knihovny.cz E-resources
- Keywords
- ELISA, Gold nanoparticles, PCR, Tau protein,
- MeSH
- Alzheimer Disease diagnosis MeSH
- Biomarkers cerebrospinal fluid MeSH
- Enzyme-Linked Immunosorbent Assay * MeSH
- Metal Nanoparticles * MeSH
- Humans MeSH
- Antibodies, Monoclonal immunology MeSH
- Polymerase Chain Reaction methods MeSH
- tau Proteins cerebrospinal fluid MeSH
- Sensitivity and Specificity MeSH
- Tyramine metabolism MeSH
- Gold * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers MeSH
- MAPT protein, human MeSH Browser
- Antibodies, Monoclonal MeSH
- tau Proteins MeSH
- Tyramine MeSH
- Gold * MeSH
Tau protein in cerebrospinal fluid (CSF) is an important biomarker of Alzheimer's disease and some other brain diseases. Enzyme-linked immunosorbent assays (ELISAs) have been mostly used for quantification of tau and other biomarkers in CSF. However, these assays do not have sufficient sensitivity and dynamic range. In this study we tested the suitability of gold nanoparticles functionalized with tau-specific monoclonal antibody and oligonucleotide template for immuno-polymerase chain reaction (Nano-iPCR) quantification of tau protein in human CSF samples and compared it with ELISA, either commercial or newly developed with tyramide signal amplification. Our data indicate that Nano-iPCR is superior in sensitivity and detection range to ELISA in tau protein detection.
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