Differential expression of anterior gradient protein 3 in intrahepatic cholangiocarcinoma and hepatocellular carcinoma
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24747240
DOI
10.1016/j.yexmp.2014.04.002
PII: S0014-4800(14)00052-5
Knihovny.cz E-zdroje
- Klíčová slova
- AGR3, GPC-3, Hepatocellular carcinoma, Immunohistochemistry, Intrahepatic cholangiocarcinoma, Mucopolysaccharides,
- MeSH
- cholangiokarcinom diagnóza genetika MeSH
- diferenciální diagnóza MeSH
- dospělí MeSH
- genetické markery MeSH
- glypikany genetika metabolismus MeSH
- hepatocelulární karcinom diagnóza genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- nádorové biomarkery genetika metabolismus MeSH
- nádorové proteiny genetika metabolismus MeSH
- nádory jater diagnóza genetika MeSH
- nádory žlučových cest MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- transportní proteiny genetika metabolismus MeSH
- žlučové cesty intrahepatální metabolismus patologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- AGR3 protein, human MeSH Prohlížeč
- genetické markery MeSH
- glypikany MeSH
- GPC3 protein, human MeSH Prohlížeč
- nádorové biomarkery MeSH
- nádorové proteiny MeSH
- transportní proteiny MeSH
Intrahepatic cholangiocarcinoma (ICC) is the second most common primary liver cancer next to hepatocellular carcinoma (HCC). Despite the significant difference of the therapeutic strategy for both diseases, their histological appearance may be very similar. Thus the correct diagnosis is crucial for treatment choice but is often difficult to achieve. The aim of our study was to evaluate anterior gradient 3 (AGR3) as a new diagnostic marker helping to distinguish between ICC and HCC. AGR3 is a putative transmembrane protein implicated in breast, prostate and ovary tumorigenesis and belongs to the family of protein disulfide isomerases. Since there is little information on how AGR3 is expressed in normal and diseased tissues and what its exact function is, we analyzed its expression pattern in normal liver and tumor tissue of ICC and HCC. The immunohistochemical analysis in normal tissue revealed specific AGR3 expression in intrahepatic bile duct cholangiocytes which was not present in liver hepatocytes. Consequently we analyzed AGR3 expression in 74 representative samples of puncture biopsies, tissue excisions and resection specimens from which 48 samples were diagnosed as HCC and 26 as ICC. Our results showed AGR3 expression negative and weakly positive respectively in hepatocellular carcinomas compared to stronger AGR3 positivity in cholangiocellular carcinomas. AGR3 expression statistically significantly correlated to acid mucopolysaccharide expression and negatively correlated to glypican-3 expression. We conclude that according to receiver operating characteristics (ROC) analysis AGR3 expression is relatively specific for ICC and is potentially linked to mucosecretion, which may indicate potential implication in treatment resistance.
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