The aim of this study was to evaluate the bioactive substances in 19 berry cultivars of edible honeysuckle (Lonicera edulis). A statistical evaluation was used to determine the relationship between the content of selected bioactive substances and individual cultivars. Regarding mineral elements, the content of sodium was measured using potentiometry and spectrophotometry. The content of selected polyphenolic compounds with high antioxidant activity was determined by a HPLC-UV/ED method. The total amount of polyphenols was determined by the Folin-Ciocalteu method. The antioxidant activity was determined using five methods (DPPH, FRAP, ABTS, FR and DMPD) that differ in their principles. The content of 13 amino acids was determined by ion-exchange chromatography. The experimental results obtained for the different cultivars were evaluated and compared by statistical and bioinformatic methods. A unique feature of this study lies in the exhaustive analysis of the chosen parameters (amino acids, mineral elements, polyphenolic compounds and antioxidant activity) during one growing season.

Department of Biomedical Engineering Faculty of Electrical Engineering and Communication Brno University of Technology Kolejni 4 CZ 612 00 Brno Czech Republic

Department of Chemistry and Biochemistry Faculty of Agronomy Mendel University Zemedelska 1 CZ 613 00 Brno Czech Republic

Department of Food Analysis and Chemistry Faculty of Technology Tomas Bata University Namesti T G Masaryka 275 762 72 Zlin Czech Republic

Department of Garden and Landscape Architecture Faculty of Horticulture Mendel University Valticka 337 CZ 691 44 Lednice Czech Republic

Department of Vegetable Science and Floriculture Faculty of Horticulture Mendel University Lednice Valtická 337 CZ 691 44 Lednice Czech Republic

Department of Viticulture and Enology Faculty of Horticulture Mendel University Valticka 337 CZ 691 44 Lednice Czech Republic

Faculty of Military Health Sciences University of Defence Trebesska 1575 CZ 500 01 Hradec Kralove Czech Republic

Institute for Teacher Training Faculty of Central European Studies Constantine the Philosopher University Drazovska 4 SK 949 74 Nitra Slovak Republic

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Plekhanova M.N., Streltsyna S.A., Rostova N.S. Fruit Chemical Composition of Lonicera subsect Caerulea (Caprifoliaceae species), Genetic Resourses in Russia and Neighbouring Countries. volume 30. Estonian Agricultural University-Forest Research Institut; Tartu, Estonia: 1998. pp. 143–146.

Pokorna-Jurikova T., Matuskovic J. The study of irrigation influence on nutritional value of Lonicera kamtschatica—Cultivar Gerda 25 and Lonicera edulis berries under the Nitra conditions during 2001–2003. Hortic. Sci. 2007;34:11–16.

Jurikova T., Rop O., Mlcek J., Sochor J., Balla S., Szekeres L., Hegedusova A., Hubalek J., Adam V., Kizek R. Phenolic profile of edible honeysuckle berries (Genus lonicera) and their biological effects. Molecules. 2012;17:61–79. PubMed PMC

Jurikova T., Sochor J., Rop O., Mlcek J., Balla S., Szekeres L., Zitny R., Zitka O., Adam V., Kizek R. Evaluation of polyphenolic profile and nutritional value of non-traditional fruit species in the Czech Republic—A comparative study. Molecules. 2012;17:8968–8981. doi: 10.3390/molecules17088968. PubMed DOI PMC

Gazdik Z., Krska B., Adam V., Saloun J., Pokorna T., Reznicek V., Horna A., Kizek R. Electrochemical determination of the antioxidant potential of some less common fruit species. Sensors. 2008;8:7564–7570. doi: 10.3390/s8127564. PubMed DOI PMC

Lefèvre I., Ziebel J., Guignard C., Sorokin A., Tikhonova O., Dolganova N., Hoffmann L., Eyzaguirre P., Hausman J.-F. Evaluation and comparison of nutritional quality and bioactive compounds of berry fruits from Lonicera caerulea, Ribes L. species and Rubus idaeus grown in Russia. J. Berry Res. 2011;1:159–167.

Rop O., Reznicek V., Mlcek J., Jurikova T., Balik J., Sochor J., Kramarova D. Antioxidant and radical oxygen species scavenging activities of 12 cultivars of blue honeysuckle fruit. Hortic. Sci. 2011;38:63–70.

Myjavcova R., Marhol P., Kren V., Simanek V., Ulrichova J., Palikova I., Papouskova B., Lemr K., Bednar P. Analysis of anthocyanin pigments in Lonicera (Caerulea) extracts using chromatographic fractionation followed by microcolumn liquid chromatography-mass spectrometry. J. Chromatogr. A. 2010;1217:7932–7941. doi: 10.1016/j.chroma.2010.05.058. PubMed DOI

Chaovanalikit A., Thompson M.M., Wrolstad R.E. Characterization and quantification of Anthocyanins and polyphenolics in blue honeysuckle (Lonicera caerulea L.) J. Agric. Food Chem. 2004;52:848–852. doi: 10.1021/jf030509o. PubMed DOI

Palikova I., Heinrich J., Bednar P., Marhol P., Kren V., Cvak L., Valentova K., Ruzicka F., Hola V., Kolar M., et al. Constituents and Antimicrobial Properties of Blue Honeysuckle: A Novel Source for Phenolic Antioxidants. J. Agric. Food Chem. 2008;56:11883–11889. doi: 10.1021/jf8026233. PubMed DOI

Farcasanu I.C., Gruia M.I., Paraschivescu C., Oprea E., Baciu I. Ethanol extracts of Lonicera caerulea and Sambucus nigra berries exhibit antifungal properties upon heat-stressed Saccharomyces cerevisiae cells. Rev. Chim. 2006;57:79–81.

Gruia M.I., Oprea E., Gruia I., Negoita V., Farcasanu I.C. The Antioxidant Response Induced by Lonicera caerulaea Berry Extracts in Animals Bearing Experimental Solid Tumors. Molecules. 2009;14:893–893. doi: 10.3390/molecules14020893. PubMed DOI PMC

Thompson M.M., Chaovanalikit A. A. Preliminary Observations on Adaptation and Nutraceutical Values of Blue Honeysuckle (Lonicera caerulea) in Oregon, USA. In: Hicklenton P., Maas J., editors. Berry Crop Breeding, Production and Utilization for a New Century. 26th International Horticultural Congress; Toronto, ON, Canada: 2003. pp. 65–72.

Zhao H.T., Wang Z.Y., Cheng C.L., Yao L., Wang L., Lu W.H., Yang X., Ma F.M. In-vitro free radical scavenging activities of anthocyanins from three berries. J. Med. Plants Res. 2011;5:7036–7042.

Kusznierewicz B., Piekarska A., Mrugalska B., Konieczka P., Namiesnik J., Bartoszek A. Phenolic composition and antioxidant properties of polish blue-berried honeysuckle genotypes by HPLC-DAD-MS, HPLC postcolumn derivatization with ABTS or FC, and TLC with DPPH visualization. J. Agric. Food Chem. 2012;60:1755–1763. doi: 10.1021/jf2039839. PubMed DOI

Švarcova I., Heinrich J., Valentova K. Berry fruits as a source of biologically active compounds: The case of Lonicera caerulea. Biomed. Pap. Med. Fac. Univ. Palacky Olomouc. 2007;151:163–174. doi: 10.5507/bp.2007.031. PubMed DOI

Skupien K., Ochmian I., Grajkowski J. Influence of ripening time on fruit chemical composition of two blue honeysuckle cultigens. J. Fruit Ornam. Plant Res. 2009;17:101–111.

Ochmian I., Skupien K., Grajkowski J., Smolik M., Ostrowska K. Chemical Composition and Physical Characteristics of Fruits of Two Cultivars of Blue Honeysuckle (Lonicera caerulea L.) in Relation to their Degree of Maturity and Harvest Date. Not. Bot. Horti Agrobot. Cluj-Napoca. 2012;40:155–162.

Rupasinghe H.P.V., Yu L.J., Bhullar K.S., Bors B. Short communication: Haskap (Lonicera caerulea): A new berry crop with high antioxidant capacity. Can. J. Plant Sci. 2012;92:1311–1317. doi: 10.4141/cjps2012-073. DOI

Streltsyna S.A., Sorotkin A.A., Plekhanova M.N., Lobanova E.V. Sostav biologičeskich aktivnyh fenolyj soedinenii sortov žimolosti v uslovjach everno-zapadnoj zony plodovodstva. Agrar. Ross. 2006;6:67–72.

Bakowska-Barczak A.M., Marianchuk M., Kolodziejczyk P. Survey of bioactive components in Western Canadian berries. Can. J. Physiol. Pharmacol. 2007;85:1139–1152. doi: 10.1139/Y07-102. PubMed DOI

Vasco C., Ruales J., Kamal-Eldin A. Total phenolic compounds and antioxidant capacities of major fruits from Ecuador. Food Chem. 2008;111:816–823. doi: 10.1016/j.foodchem.2008.04.054. DOI

Plekhanova M.N. Blue Honeysuckle (Lonicera caerulea L.)—A New Commercial Berry Crop for Temperate Climate: Genetic Resources and Breeding. In: Geibel M., Fischer M., Fischer C., editors. Proceedings of the Eucarpia Symposium on Fruit Breeding and Genetics; Dresden, Germany. 6–10 September 1999; Leuven, Belgium: International Society Horticultural Science; 2000. pp. 159–164.

Zadernowski R., Naczk M., Nesterowicz J. Phenolic acid profiles in some small berries. J. Agric. Food Chem. 2005;53:2118–2124. doi: 10.1021/jf040411p. PubMed DOI

Orincak J., Matuskovic J., Jurcak S. Possibilities of Species Lonicera caerulea in Utilization of the Secondary Metabolism in Food and Pharmaceutical Processing. 1st ed. SPU; Nitra, Slovakia: 2003. pp. 210–219.

Deineka V.I., Sorokopudov V.N., Deineka L.A., Shaposhnik E.I., Koltsov S.V. Anthocyans from fruit of some plants of the Caprifoliaceae family. Chem. Nat. Compd. 2005;41:162–164. doi: 10.1007/s10600-005-0102-2. DOI

Manach C., Scalbert A., Morand C., Remesy C., Jimenez L. Polyphenols: Food sources and bioavailability. Am. J. Clin. Nutr. 2004;79:727–747. PubMed

Oszmianski J., Wojdylo A. Aronia melanocarpa phenolics and their antioxidant activity. Eur. Food Res. Technol. 2005;221:809–813. doi: 10.1007/s00217-005-0002-5. DOI

Li H.Y., Deng Z.Y., Wu T., Liu R.H., Loewen S., Tsao R. Microwave-assisted extraction of phenolics with maximal antioxidant activities in tomatoes. Food Chem. 2012;130:928–936. doi: 10.1016/j.foodchem.2011.08.019. DOI

Jurikova T., Matuskovic J., Gazdik Z. Effect of irrigation on intensity of respiration and study of sugar and organic acids content in different development stages of Lonicera kamtschatica and Lonicera edulis berries. Hortic. Sci. 2009;36:14–20.

Gazdik Z., Reznicek V., Adam V., Zitka O., Jurikova T., Krska B., Matuskovic J., Plsek J., Saloun J., Horna A., et al. Use of Liquid Chromatography with Electrochemical Detection for the Determination of Antioxidants in Less Common Fruits. Molecules. 2008;13:2823–2836. doi: 10.3390/molecules131102823. PubMed DOI PMC

Ochmian I., Grajkowski J., Skupien K. Field performance, fruit chemical composition and formness under cold storage and stimulated “shelf–life” conditions of tree blue honeysuckle cultigens (Lonicera caerulea) J. Fruit Ornam. Plant Res. 2008;16:83–91.

Hakkinen S.H., Karenlampi S.O., Heinonen I.M., Mykkanen H.M., Torronen A.R. HPLC method for screening of flavonoids and phenolic acids in berries. J. Sci. Food Agric. 1998;77:543–551. doi: 10.1002/(SICI)1097-0010(199808)77:4<543::AID-JSFA78>3.0.CO;2-I. DOI

Jurikova T., Sochor J., Rop O., Mlcek J., Balla S., Szekeres L., Adam V., Kizek R. Polyphenolic profile and biological activity of Chinese hawthorn (Crataegus pinnatifida BUNGE) fruits. Molecules. 2012;17:14490–14509. doi: 10.3390/molecules171214490. PubMed DOI PMC

Smolik M., Ochmian I., Grajkowski J. Genetic variability of Polish and Russian accessions of cultivated blue honeysuckle (Lonicera caerulea) Russ. J. Genet. 2010;46:960–966. doi: 10.1134/S1022795410080077. PubMed DOI

Benzie I.F.F., Strain J.J. Ferric reducing antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol. 1999;299:15–27. doi: 10.1016/S0076-6879(99)99005-5. PubMed DOI

Wang H., Cao G.H., Prior R.L. Total antioxidant capacity of fruits. J. Agric. Food Chem. 1996;44:701–705. doi: 10.1021/jf950579y. DOI

Rop O., Posolda M., Mlcek J., Reznicek V., Sochor J., Adam V., Kizek R., Sumczynski D. Qualities of native apple cultivar juices characteristic of Central Europe. Not. Bot. Horti Agrobot. Cluj-Napoca. 2012;40:222–228.

Rop O., Sochor J., Jurikova T., Zitka O., Skutkova H., Mlcek J., Salas P., Krska B., Babula P., Adam V., et al. Effect of five different stages of ripening on chemical compounds in medlar (Mespilus germanica L.) Molecules. 2011;16:74–91. PubMed PMC

Sochor J., Skutkova H., Babula P., Zitka O., Cernei N., Rop O., Krska B., Adam V., Provaznik I., Kizek R. Mathematical Evaluation of the Amino Acid and Polyphenol Content and Antioxidant Activities of Fruits from Different Apricot Cultivars. Molecules. 2011;16:7428–7457. doi: 10.3390/molecules16097428. PubMed DOI PMC

Rop O., Balik J., Reznicek V., Jurikova T., Skardova P., Salas P., Sochor J., Mlcek J., Kramarova D. Chemical characteristics of fruits of some selected quince (Cydonia oblonga Mill.) cultivars. Czech J. Food Sci. 2011;29:65–73.

Rop O., Jurikova T., Sochor J., Mlcek J., Kramarova D. Antioxidant capacity, scavenging radical activity and selected chemical composition of native apple cultivars from central Europe. J. Food Qual. 2011;34:187–194. doi: 10.1111/j.1745-4557.2011.00387.x. DOI

Koracevic D., Koracevic G., Djordjevic V., Andrejevic S., Cosic V. Method for the measurement of antioxidant activity in human fluids. J. Clin. Pathol. 2001;54:356–361. doi: 10.1136/jcp.54.5.356. PubMed DOI PMC

Sochor J., Babula P., Krska B., Horna A., Provaznik I., Hubalek J., Kizek R. Evaluation of output signals from CoulArray detector for determination of antioxidant capacity of apricots samples. In: Jan J., Jirik R., Kolar R., Kolarova J., Kozumplik J., Provaznik I., editors. Analysis of Biomedical Signals and Images. Brno Univ Technology Vut Press; Brno, Czech Republic: 2010. pp. 209–214.

Prior R.L., Wu X.L., Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J. Agric. Food Chem. 2005;53:4290–4302. doi: 10.1021/jf0502698. PubMed DOI

Pohanka M., Stetina R., Svobodova H., Ruttkay-Nedecky B., Jilkova M., Sochor J., Sobotka J., Adam V., Kizek R. Sulfur mustard causes oxidative stress and depletion of antioxidants in muscles, livers, and kidneys of Wistar rats. Drug Chem. Toxicol. 2013;36:270–276. doi: 10.3109/01480545.2012.710629. PubMed DOI

Pohanka M., Sochor J., Ruttkay-Nedecky B., Cernei N., Adam V., Hubalek J., Stiborova M., Eckschlager T., Kizek R. Automated assay of the potency of natural antioxidants using pipetting robot and spectrophotometry. J. Appl. Biomed. 2012;10:155–167. doi: 10.2478/v10136-012-0006-y. DOI

Raudsepp P., Anton D., Roasto M., Meremae K., Pedastsaar P., Maesaar M., Raal A., Laikoja K., Pussa T. The antioxidative and antimicrobial properties of the blue honeysuckle (Lonicera caerulea L.), Siberian rhubarb (Rheum rhaponticum L.) and some other plants, compared to ascorbic acid and sodium nitrite. Food Control. 2013;31:129–135. doi: 10.1016/j.foodcont.2012.10.007. DOI

Paulovicsova B., Turianica I., Jurikova T., Baloghova M., Matuskovic J. Antioxidant properties of selected less common fruit species. Lucrări ştiinţifice Zootehnie şi Biotehnologii. 2009;42:608–614.

Lee J., Schreiner R.P. Free amino acid profiles from “Pinot noir” grapes are influenced by vine N-status and sample preparation method. Food Chem. 2010;119:484–489. doi: 10.1016/j.foodchem.2009.06.045. DOI

Wallrauch S. Amino-acids criteria for the evaluation of fruit juices. Abstr. Pap. Am. Chem. Soc. 1985;189:0065–7727.

Zitka O., Sochor J., Rop O., Skalickova S., Sobrova P., Zehnnalek J., Beklova M., Krska B., Adam V., Kizek R. Comparison of various easy-to-use procedures for extraction of phenols from apricot fruits. Molecules. 2011;16:2914–2936. doi: 10.3390/molecules16042914. PubMed DOI PMC

Singleton V.L., Orthofer R., Lamuela-Raventos R.M. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In: Packer L., editor. Oxidants and Antioxidants, Part A. Gulf Professional Publishing; San Diego, CA, USA: 1999. pp. 152–178.

Sochor J., Ryvolova M., Krystofova O., Salas P., Hubalek J., Adam V., Trnkova L., Havel L., Beklova M., Zehnalek J., et al. Fully automated spectrometric protocols for determination of antioxidant activity: Advantages and disadvantages. Molecules. 2010;15:8618–8640. doi: 10.3390/molecules15128618. PubMed DOI PMC

Sochor J., Salas P., Zehnalek J., Krska B., Adam V., Havel L., Kizek R. An assay for spectrometric determination of antioxidant activity of a biological extract. Listy Cukrov. 2010;126:416–417.

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