Different phase behavior and packing of ceramides with long (C16) and very long (C24) acyls in model membranes: infrared spectroscopy using deuterated lipids
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25122563
DOI
10.1021/jp506407r
Knihovny.cz E-resources
- MeSH
- Models, Biological MeSH
- Ceramides chemistry MeSH
- Cholesterol chemistry MeSH
- Epidermis chemistry MeSH
- Cholesterol Esters chemistry MeSH
- Fatty Acids chemistry MeSH
- Membranes chemistry MeSH
- Models, Molecular MeSH
- Spectrophotometry, Infrared MeSH
- Temperature MeSH
- Humidity MeSH
- Hydrogen Bonding MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Ceramides MeSH
- Cholesterol MeSH
- cholesteryl sulfate MeSH Browser
- Cholesterol Esters MeSH
- lignoceric acid MeSH Browser
- Fatty Acids MeSH
Ceramides (Cer) are the central molecules in sphingolipid metabolism that participate in cellular signaling and also prevent excessive water loss by the skin. Previous studies showed that sphingosine-based Cer with a long 16C chain (CerNS16) and very long 24C-chain ceramides (CerNS24) differ in their biological actions. Increased levels of long CerNS16 at the expense of the very long CerNS24 have been found in atopic dermatitis patients, and this change correlated with the skin barrier properties. To probe the membrane behavior of the long CerNS16 and the very long chain CerNS24, we studied their interactions with fatty acids and cholesterol in model stratum corneum membranes using infrared spectroscopy. Using Cer with deuterated acyls and/or deuterated fatty acids, we showed differences in lipid mixing, packing, and thermotropic phase behavior between long and very long Cer. These differences were observed in the presence of lignoceric acid or a heterogeneous fatty acid mixture (C16-C24), in the presence or absence of cholesterol sulfate, and at 5-95% humidity. In these membranes, very long CerNS24 prefers an extended (splayed-chain) conformation in which the fatty acid is associated with the very long Cer chain. In contrast, the shorter CerNS16 and fatty acids are mostly phase separated.
References provided by Crossref.org
Lysosphingolipids in ceramide-deficient skin lipid models
Polymorphism, Nanostructures, and Barrier Properties of Ceramide-Based Lipid Films
Behavior of 1-Deoxy-, 3-Deoxy- and N-Methyl-Ceramides in Skin Barrier Lipid Models
Long and very long lamellar phases in model stratum corneum lipid membranes