Different phase behavior and packing of ceramides with long (C16) and very long (C24) acyls in model membranes: infrared spectroscopy using deuterated lipids
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25122563
DOI
10.1021/jp506407r
Knihovny.cz E-zdroje
- MeSH
- biologické modely MeSH
- ceramidy chemie MeSH
- cholesterol chemie MeSH
- epidermis chemie MeSH
- estery cholesterolu chemie MeSH
- mastné kyseliny chemie MeSH
- membrány chemie MeSH
- molekulární modely MeSH
- spektrofotometrie infračervená MeSH
- teplota MeSH
- vlhkost MeSH
- vodíková vazba MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ceramidy MeSH
- cholesterol MeSH
- cholesteryl sulfate MeSH Prohlížeč
- estery cholesterolu MeSH
- lignoceric acid MeSH Prohlížeč
- mastné kyseliny MeSH
Ceramides (Cer) are the central molecules in sphingolipid metabolism that participate in cellular signaling and also prevent excessive water loss by the skin. Previous studies showed that sphingosine-based Cer with a long 16C chain (CerNS16) and very long 24C-chain ceramides (CerNS24) differ in their biological actions. Increased levels of long CerNS16 at the expense of the very long CerNS24 have been found in atopic dermatitis patients, and this change correlated with the skin barrier properties. To probe the membrane behavior of the long CerNS16 and the very long chain CerNS24, we studied their interactions with fatty acids and cholesterol in model stratum corneum membranes using infrared spectroscopy. Using Cer with deuterated acyls and/or deuterated fatty acids, we showed differences in lipid mixing, packing, and thermotropic phase behavior between long and very long Cer. These differences were observed in the presence of lignoceric acid or a heterogeneous fatty acid mixture (C16-C24), in the presence or absence of cholesterol sulfate, and at 5-95% humidity. In these membranes, very long CerNS24 prefers an extended (splayed-chain) conformation in which the fatty acid is associated with the very long Cer chain. In contrast, the shorter CerNS16 and fatty acids are mostly phase separated.
Citace poskytuje Crossref.org
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