Cytokinin response factors regulate PIN-FORMED auxin transporters
Language English Country England, Great Britain Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26541513
DOI
10.1038/ncomms9717
PII: ncomms9717
Knihovny.cz E-resources
- MeSH
- Arabidopsis MeSH
- Chromatin Immunoprecipitation MeSH
- Cytokinins metabolism MeSH
- Plants, Genetically Modified MeSH
- Microscopy, Confocal MeSH
- Plant Roots metabolism MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Indoleacetic Acids metabolism MeSH
- Membrane Transport Proteins genetics metabolism MeSH
- Arabidopsis Proteins genetics metabolism MeSH
- Gene Expression Regulation, Plant MeSH
- Response Elements MeSH
- Signal Transduction MeSH
- Transcription Factors genetics metabolism MeSH
- Green Fluorescent Proteins MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CRF3 protein, Arabidopsis MeSH Browser
- CRF6 protein, Arabidopsis MeSH Browser
- Cytokinins MeSH
- Indoleacetic Acids MeSH
- Membrane Transport Proteins MeSH
- PIN1 protein, Arabidopsis MeSH Browser
- PIN7 protein, Arabidopsis MeSH Browser
- Arabidopsis Proteins MeSH
- Transcription Factors MeSH
- Green Fluorescent Proteins MeSH
Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation of auxin distribution is a developmentally crucial hormonal interaction, its molecular basis is largely unknown. Here we show a direct regulatory link between cytokinin signalling and the auxin transport machinery uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory element effectively uncouples PIN transcription from the CRF-mediated cytokinin regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development.
Department of Plant Systems Biology VIB Technologiepark 927 B 9052 Gent Belgium
Institute of Experimental Botany ASCR Rozvojová 263 16502 Prague Czech Republic
Institute of Science and Technology Austria Am Campus 1 3400 Klosterneuburg Austria
Laboratory of Biochemistry Wageningen University Dreijenlaan 3 6703HA Wageningen the Netherlands
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