Molecular identification of Entamoeba species in savanna woodland chimpanzees (Pan troglodytes schweinfurthii)
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26935395
DOI
10.1017/s0031182016000263
PII: S0031182016000263
Knihovny.cz E-zdroje
- Klíčová slova
- Entamoeba, chimpanzee, great apes, molecular diversity, savannah,
- MeSH
- entamébóza epidemiologie parazitologie MeSH
- Entamoeba klasifikace genetika izolace a purifikace MeSH
- feces parazitologie MeSH
- fylogeneze MeSH
- nemoci lidoopů epidemiologie parazitologie MeSH
- Pan troglodytes parazitologie MeSH
- pastviny MeSH
- polymerázová řetězová reakce MeSH
- protozoální DNA genetika MeSH
- sekvenční homologie nukleových kyselin MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Tanzanie epidemiologie MeSH
- Názvy látek
- protozoální DNA MeSH
To address the molecular diversity and occurrence of pathogenic species of the genus Entamoeba spp. in wild non-human primates (NHP) we conducted molecular-phylogenetic analyses on Entamoeba from wild chimpanzees living in the Issa Valley, Tanzania. We compared the sensitivity of molecular [using a genus-specific polymerase chain reaction (PCR)] and coproscopic detection (merthiolate-iodine-formaldehyde concentration) of Entamoeba spp. We identified Entamoeba spp. in 72 chimpanzee fecal samples (79%) subjected to species-specific PCRs for six Entamoeba species/groups (Entamoeba histolytica, Entamoeba nuttalli, Entamoeba dispar, Entamoeba moshkovskii, Entamoeba coli and Entamoeba polecki ST2). We recorded three Entamoeba species: E. coli (47%), E. dispar (16%), Entamoeba hartmanni (51%). Coproscopically, we could only distinguish the cysts of complex E. histolytica/dispar/moshkovskii/nuttalli and E. coli. Molecular prevalence of entamoebas was higher than the prevalence based on the coproscopic examination. Our molecular phylogenies showed that sequences of E. dispar and E. coli from Issa chimpanzees are closely related to sequences from humans and other NHP from GenBank. The results showed that wild chimpanzees harbour Entamoeba species similar to those occurring in humans; however, no pathogenic species were detected. Molecular-phylogenetic methods are critical to improve diagnostics of entamoebas in wild NHP and for determining an accurate prevalence of Entamoeba species.
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