Results from a round robin test for the ecotoxicological evaluation of construction products using two leaching tests and an aquatic test battery
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
PubMed
28211327
DOI
10.1016/j.chemosphere.2017.01.146
PII: S0045-6535(17)30165-0
Knihovny.cz E-resources
- Keywords
- Construction products, Ecotoxicity tests, Eluates, Leaching tests, Round robin test,
- MeSH
- Bacteria drug effects MeSH
- Biological Assay methods standards MeSH
- Water Pollutants, Chemical analysis toxicity MeSH
- Zebrafish MeSH
- Daphnia drug effects MeSH
- Ecotoxicology methods standards MeSH
- Elastomers toxicity MeSH
- Ethylenes toxicity MeSH
- Rubber toxicity MeSH
- Stramenopiles drug effects MeSH
- Observer Variation MeSH
- Reproducibility of Results MeSH
- Toxicity Tests methods standards MeSH
- Eggs MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Water Pollutants, Chemical MeSH
- Elastomers MeSH
- ethylene-propylene-diene-monomer MeSH Browser
- Ethylenes MeSH
- Rubber MeSH
A European round robin test according to ISO 5725-2 was conceptually prepared, realised, and evaluated. The aim was to determine the inter-laboratory variability of the overall process for the ecotoxicological characterization of construction products in eluates and bioassays. To this end, two construction products BAM-G1 (granulate) and HSR-2 (roof sealing sheet), both made of EPDM polymers (rubber), were selected. The granular construction product was eluted in a one stage batch test, the planar product in the Dynamic Surface Leaching test (DSLT). A total of 17 laboratories from 5 countries participated in the round robin test: Germany (12), Austria (2), Belgium (1), Czech Republic (1) and France (1). A test battery of four standardised ecotoxicity tests with algae, daphnia, luminescent bacteria and zebrafish eggs was used. As toxicity measures, EC50 and LID values were calculated. All tests, except the fish egg test, were basically able to demonstrate toxic effects and the level of toxicity. The reproducibility of test results depended on the test specimens and the test organisms. Generally, the variability of the EC50 or LID values increased with the overall level of toxicity. For the very toxic BAM-G1 eluate a relative high variability of CV = 73%-110% was observed for EC50 in all biotests, while for the less toxic HSR-2 eluate the reproducibility of EC50 varied with sensitivity: it was very good (CV = 9.3%) for the daphnia test with the lowest sensitivity, followed by the algae test (CV = 36.4%). The luminescent bacteria test, being the most sensitive bioassay for HSR-2 Eluate, showed the highest variability (CV = 74.8%). When considering the complex overall process the reproducibility of bioassays with eluates from construction products was acceptable.
BAM Federal Institute for Materials Research and Testing Berlin Germany
Dr U Noack Laboratorien Sarstedt Germany
ECT Oekotoxikologie GmbH Flörsheim Main Germany
Eurofins Agroscience Services Ecotox GmbH Niefern Öschelbronn Germany
Federal Environment Agency Dessau Germany
Federal Environment Agency FG 3 2 5 Berlin Germany
Federal Institute of Hydrology Koblenz Germany
Hydrotox GmbH Freiburg Germany
IDUS Biologisch Analytisches Umweltlabor Ottendorf Okrilla Germany
INERIS Verneuil en Halatte France
Institut Dr Nowak Ottersberg Germany
Lenzing AG Safety Health and Environment Department Lenzing Austria
Masaryk Water Research Institute Ostrava Czechia
Niedersächsischer Landesbetrieb für Wasserwirtschaft Küsten und Naturschutz Hildesheim Germany
SGS Institut Fresenius GmbH Taunusstein Germany
ToxRat Solutions GmbH Alsdorf Germany
University of Natural Resources and Life Sciences Vienna IFA Tulln Austria
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