Low-pH induced reversible reorganizations of chloroplast thylakoid membranes - As revealed by small-angle neutron scattering
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
28237493
DOI
10.1016/j.bbabio.2017.02.010
PII: S0005-2728(17)30038-5
Knihovny.cz E-resources
- Keywords
- Chloroplast thylakoid membranes, Lamellar repeat distance, Low pH and p(2)H, Small-angle neutron scattering (SANS),
- MeSH
- Membrane Fluidity MeSH
- Photosynthesis * MeSH
- Photosystem I Protein Complex metabolism ultrastructure MeSH
- Photosystem II Protein Complex metabolism ultrastructure MeSH
- Pisum sativum metabolism ultrastructure MeSH
- Hydrogen-Ion Concentration MeSH
- Plant Leaves metabolism MeSH
- Scattering, Small Angle * MeSH
- Neutron Diffraction * MeSH
- Energy Transfer MeSH
- Thylakoids metabolism ultrastructure MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Photosystem I Protein Complex MeSH
- Photosystem II Protein Complex MeSH
Energization of thylakoid membranes brings about the acidification of the lumenal aqueous phase, which activates important regulatory mechanisms. Earlier Jajoo and coworkers (2014 FEBS Lett. 588:970) have shown that low pH in isolated plant thylakoid membranes induces changes in the excitation energy distribution between the two photosystems. In order to elucidate the structural background of these changes, we used small-angle neutron scattering on thylakoid membranes exposed to low p2H (pD) and show that gradually lowering the p2H from 8.0 to 5.0 causes small but well discernible reversible diminishment of the periodic order and the lamellar repeat distance and an increased mosaicity - similar to the effects elicited by light-induced acidification of the lumen. Our data strongly suggest that thylakoids dynamically respond to the membrane energization and actively participate in different regulatory mechanisms.
College of Nyíregyháza Institute of Environmental Science H 4400 Nyíregyháza Hungary
Paul Scherrer Institute Laboratory for Neutron Scattering and Imaging 5232 Villigen PSI Switzerland
School of Life Science Devi Ahilya University Khandwa Road Indore 452 001 India
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