Deregulated expression of long non-coding RNA UCA1 in multiple myeloma
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
28543758
DOI
10.1111/ejh.12908
Knihovny.cz E-zdroje
- Klíčová slova
- biomarker, long non-coding RNA, multiple myeloma, plasma cells, prognosis, quantitative real-time PCR,
- MeSH
- biologické markery MeSH
- chromozomální aberace MeSH
- diferenciální diagnóza MeSH
- hybridizace in situ fluorescenční MeSH
- Kaplanův-Meierův odhad MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé středního věku MeSH
- lidé MeSH
- mnohočetný myelom diagnóza genetika mortalita terapie MeSH
- nádorové biomarkery * MeSH
- prognóza MeSH
- regulace genové exprese u nádorů * MeSH
- RNA dlouhá nekódující genetika MeSH
- ROC křivka MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- staging nádorů MeSH
- stanovení celkové genové exprese MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- biologické markery MeSH
- nádorové biomarkery * MeSH
- NEAT1 long non-coding RNA, human MeSH Prohlížeč
- RNA dlouhá nekódující MeSH
- UCA1 RNA, human MeSH Prohlížeč
OBJECTIVES: Long non-coding RNAs (lncRNAs) are RNA transcripts longer than 200 nucleotides that are not translated into proteins. They are involved in pathogenesis of many diseases including cancer and have a potential to serve as diagnostic and prognostic markers. We aimed to investigate lncRNA expression profiles in bone marrow plasma cells (BMPCs) of newly diagnosed multiple myeloma (MM) patients in comparison to normal BMPCs of healthy donors (HD) in a three-phase biomarker study. METHODS: Expression profile of 83 lncRNA was performed by RT2 lncRNA PCR Array (Qiagen), followed by quantitative real-time PCR using specific TaqMan non-coding RNA assays analyzing 84 newly diagnosed MM patients and 25 HD. RESULTS: Our analysis revealed dysregulation of two lncRNAs; NEAT1 (sensitivity of 55.0% and specificity of 79.0%) and UCA1 (sensitivity of 85.0% and specificity of 94.7%). UCA1 levels correlated with albumin and monoclonal immunoglobulin serum levels, cytogenetic aberrations, and survival of MM patients. CONCLUSION: Our study suggests a possible prognostic impact of UCA1 expression levels on MM patients.
Central European Institute of Technology Masaryk University Brno Czech Republic
Department of Clinical Hematology University Hospital Brno Brno Czech Republic
Department of Experimental Biology Faculty of Science Masaryk University Brno Czech Republic
Department of Hematooncology University Hospital Ostrava Ostrava Czech Republic
Institute of Biostatistics and Analyses Faculty of Medicine Masaryk University Brno Czech Republic
Citace poskytuje Crossref.org
Long Non-Coding RNAs in Multiple Myeloma
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