Deregulated expression of long non-coding RNA UCA1 in multiple myeloma
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
28543758
DOI
10.1111/ejh.12908
Knihovny.cz E-resources
- Keywords
- biomarker, long non-coding RNA, multiple myeloma, plasma cells, prognosis, quantitative real-time PCR,
- MeSH
- Biomarkers MeSH
- Chromosome Aberrations MeSH
- Diagnosis, Differential MeSH
- In Situ Hybridization, Fluorescence MeSH
- Kaplan-Meier Estimate MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Middle Aged MeSH
- Humans MeSH
- Multiple Myeloma diagnosis genetics mortality therapy MeSH
- Biomarkers, Tumor * MeSH
- Prognosis MeSH
- Gene Expression Regulation, Neoplastic * MeSH
- RNA, Long Noncoding genetics MeSH
- ROC Curve MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Neoplasm Staging MeSH
- Gene Expression Profiling MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Biomarkers MeSH
- Biomarkers, Tumor * MeSH
- NEAT1 long non-coding RNA, human MeSH Browser
- RNA, Long Noncoding MeSH
- UCA1 RNA, human MeSH Browser
OBJECTIVES: Long non-coding RNAs (lncRNAs) are RNA transcripts longer than 200 nucleotides that are not translated into proteins. They are involved in pathogenesis of many diseases including cancer and have a potential to serve as diagnostic and prognostic markers. We aimed to investigate lncRNA expression profiles in bone marrow plasma cells (BMPCs) of newly diagnosed multiple myeloma (MM) patients in comparison to normal BMPCs of healthy donors (HD) in a three-phase biomarker study. METHODS: Expression profile of 83 lncRNA was performed by RT2 lncRNA PCR Array (Qiagen), followed by quantitative real-time PCR using specific TaqMan non-coding RNA assays analyzing 84 newly diagnosed MM patients and 25 HD. RESULTS: Our analysis revealed dysregulation of two lncRNAs; NEAT1 (sensitivity of 55.0% and specificity of 79.0%) and UCA1 (sensitivity of 85.0% and specificity of 94.7%). UCA1 levels correlated with albumin and monoclonal immunoglobulin serum levels, cytogenetic aberrations, and survival of MM patients. CONCLUSION: Our study suggests a possible prognostic impact of UCA1 expression levels on MM patients.
Central European Institute of Technology Masaryk University Brno Czech Republic
Department of Clinical Hematology University Hospital Brno Brno Czech Republic
Department of Experimental Biology Faculty of Science Masaryk University Brno Czech Republic
Department of Hematooncology University Hospital Ostrava Ostrava Czech Republic
Institute of Biostatistics and Analyses Faculty of Medicine Masaryk University Brno Czech Republic
References provided by Crossref.org
Long Non-Coding RNAs in Multiple Myeloma
Circulating exosomal long noncoding RNA PRINS-First findings in monoclonal gammopathies
