Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis
Language English Country Switzerland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
29258075
DOI
10.1159/000484524
PII: 000484524
Knihovny.cz E-resources
- Keywords
- Catheter, Complex sample, Mixed chromatogram, PCR denaturing gradient gel electrophoresis, RipSeq Mixed,
- MeSH
- Bacteria classification genetics isolation & purification MeSH
- Denaturing Gradient Gel Electrophoresis methods MeSH
- Molecular Diagnostic Techniques methods MeSH
- Diagnostic Techniques, Urological MeSH
- DNA, Bacterial MeSH
- Humans MeSH
- Urine chemistry microbiology MeSH
- Urinary Catheters microbiology MeSH
- Polymerase Chain Reaction methods MeSH
- Software * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
Complex samples are a challenge for sequencing-based broad-range diagnostics. We analysed 19 urinary catheter, ureteral Double-J catheter, and urine samples using 3 methodological approaches. Out of the total 84 operational taxonomic units, 37, 61, and 88% were identified by culture, PCR-DGGE-SS (PCR denaturing gradient gel electrophoresis followed by Sanger sequencing), and PCR-DGGE-RM (PCR- DGGE combined with software chromatogram separation by RipSeq Mixed tool), respectively. The latter approach was shown to be an efficient tool to complement culture in complex sample assessment.
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