Bioinformatics analyses and in vitro evidence for five and six stacked G-quadruplex forming sequences
Language English Country France Media print-electronic
Document type Journal Article
PubMed
29733879
DOI
10.1016/j.biochi.2018.05.002
PII: S0300-9084(18)30125-1
Knihovny.cz E-resources
- Keywords
- Bioinformatics, Circular dichroism, Electrophoresis, G-quadruplex,
- MeSH
- Circular Dichroism MeSH
- G-Quadruplexes * MeSH
- Nucleic Acid Conformation MeSH
- Computational Biology methods MeSH
- Publication type
- Journal Article MeSH
Quadruplexes are noncanonical DNA structures that arise in guanine rich loci and have important biological functions. Classically, quadruplexes contain four stacked intramolecular G-tetrads. Surprisingly, although some algorithms allow searching for longer than 4G tracts for quadruplex formation, these have not yet been systematically studied. Therefore, we analyzed the human genome for sequences that are predicted to adopt stacked intramolecular G-tetrads with greater than four stacks. The data provide evidence for numerous G-quadruplexes that contain five or six stacked intramolecular G-tetrads. These sequences are predominantly found in known gene regulatory regions. Electrophoretic mobility assays and circular dichroism spectroscopy indicate that these sequences form quadruplex structures in vitro under physiological conditions. The localization and in vitro stability of these G-quadruplexes indicate their potentially important roles in gene regulation and their potential for therapeutic applications.
References provided by Crossref.org
G-Quadruplex in Gene Encoding Large Subunit of Plant RNA Polymerase II: A Billion-Year-Old Story
G-Quadruplexes in the Archaea Domain
