A series of 116 small-molecule 1-hydroxynaphthalene-2-carboxanilides was designed based on the fragment-based approach and was synthesized according to the microwave-assisted protocol. The biological activity of all of the compounds was tested on human colon carcinoma cell lines including a deleted TP53 tumor suppressor gene. The mechanism of activity was studied according to the p53 status in the cell. Several compounds revealed a good to excellent activity that was similar to or better than the standard anticancer drugs. Some of these appeared to be more active against the p53 null cells than their wild-type counterparts. Intercalating the properties of these compounds could be responsible for their mechanism of action.

A Chełkowski Institute of Physics and Silesian Center for Education and Interdisciplinary Research University of Silesia 75 Pułku Piechoty 1a 41 500 Chorzów Poland

Central Laboratories Faculty of Chemical and Food Technology Slovak University of Technology in Bratislava Radlinskeho 9 Bratislava 81237 Slovakia

Department of Analytical Chemistry Faculty of Natural Sciences Comenius University Ilkovicova 6 842 15 Bratislava Slovakia

Department of Chemical Drugs Faculty of Pharmacy University of Veterinary and Pharmaceutical Sciences Palackeho 1 Brno 612 42 Czech Republic

Department of Pharmaceutical Chemistry Faculty of Pharmacy Comenius University Odbojarov 10 832 32 Bratislava Slovakia

Global Change Research Institute CAS Belidla 986 4a Brno 603 00 Czech Republic

Institute of Chemistry University of Silesia 75 Pułku Piechoty 1a 41 500 Chorzów Poland

Regional Centre of Advanced Technologies and Materials Faculty of Science Palacky University Slechtitelu 27 783 71 Olomouc Czech Republic

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