The aim of this work was to study the potential of selected Halomonas species for conversion of waste frying oil into polyhydroxyalkanoates (PHA). In total nine Halomonas strains were experimentally screened for their capability of PHA production. Among them, Halomonas neptunia and Halomonas hydrothermalis were identified as potent PHA producers. Initial concentration of NaCl was identified as parameter influencing PHA yields as well as molecular weight of the polymer. In addition, H. hydrothermalis was capable of biosynthesis of a copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate P(3HB-co-3HV). When valerate was utilized as a precursor, the 3HV fraction in the copolymer reached high values of 50.15 mol.%. PHA production on lipid substrates by Halomonas has not been reported so far. Bearing in mind all the positive aspects of employing extremophiles in industrial biotechnology, H. hydrothermalis seems to be a very interesting halophilic strain for production of PHA using lipid substrates.

Biology Centre The Czech Academy of Sciences v v i Branisovska 31 370 05 Ceske Budejovice Czech Republic; University of South Bohemia Faculty of Science Branisovska 31 370 05 Ceske Budejovice Czech Republic

Faculty of Chemistry Brno University of Technology Purkynova 118 612 00 Brno Czech Republic

Institute of Chemistry NAWI Graz University of Graz Heinrichstrasse 28 3 8010 Graz Austria; ARENA Arbeitsgemeinschaft für Ressourcenschonende and Nachhaltige Technologien Inffeldgasse 21b 8010 Graz Austria

Citace poskytuje Crossref.org

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