Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články
Grantová podpora
109965/Z/15/Z
Wellcome Trust - United Kingdom
MR/P011136/1
Medical Research Council - United Kingdom
R50 CA211108
NCI NIH HHS - United States
PubMed
31633216
PubMed Central
PMC7350392
DOI
10.1002/eji.201970107
Knihovny.cz E-zdroje
- MeSH
- alergologie a imunologie normy MeSH
- fenotyp MeSH
- konsensus MeSH
- lidé MeSH
- průtoková cytometrie metody normy MeSH
- separace buněk metody normy MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
A·P·E Applied Physics and Electronics Berlin Germany
Alicante Institute for Health and Biomedical Research Alicante Spain
AsedaSciences West Lafayette IN USA
Barts and The London School of Medicine and Dentistry Queen Mary University of London UK
BC Children's Hospital Research Institute Vancouver Canada
BD Biosciences San Jose CA USA
BD Life Sciences Ashland OR USA
BD Life Sciences San Diego CA USA
Beckman Coulter Inc Miami FL USA
Berlin Institute of Health Berlin Germany
Bindley Biosciences Center Purdue University West Lafayette IN USA
Biophysics R and D Engineering Miltenyi Biotec GmbH Bergisch Gladbach Germany
Burnet Institute AMREP Flow Cytometry Core Facility Melbourne Victoria Australia
Cancer and Stem Cell Biology Duke NUS Medical School Singapore
Cancer Immunology Research Genentech South San Francisco CA USA
Caprion Biosciences ImmuneCarta Montréal Quebec Canada
Cell Biology Unit IRCCS Ospedale Policlinico San Martino Genova Italy
Cell Clearance in Health and Disease Lab VIB Center for Inflammation Research Ghent Belgium
Center for Immunology University of Minnesota Minneapolis MN USA
Center for Life Nano Istituto Italiano di Tecnologia Rome Italy
Centre d'Immunophénomique CIPHE Marseille France
Centre de Recherches des Cordeliers Equipe Cancer et Immunité anti tumorale Paris France
Centre for Environmental Research UFZ Department Environmental Microbiology Leipzig Germany
Chair for Immunology University Regensburg Germany
Christine Kühne Center for Allergy Research and Education Davos Switzerland
Chromocyte Limited Electric Works Sheffield UK
Clinic for Orthopedics and Trauma Surgery University Hospital Bonn Bonn Germany
Clinical Research Unit Berlin Institute of Health Charite Universitätsmedizin Berlin Berlin Germany
Clinical Trial Center Leipzig University Leipzig Leipzig Germany
Comprehensive Cancer Center Zurich Switzerland
Computer Graphics and Visualization Department of Intelligent Systems TU Delft Delft The Netherlands
Core Facility Flow Cytometry Biomedical Center Ludwig Maximilians University Munich Germany
Cytomics Technological Service Principe Felipe Research Center Valencia Spain
Data Mining and Modeling for Biomedicine VIB UGent Center for Inflammation Research Ghent Belgium
Department of Applied Mathematics Computer Science and Statistics Ghent University Ghent Belgium
Department of Biochemistry and Molecular Biology Monash University Clayton Victoria Australia
Department of Biochemistry Institute of Chemistry University of São Paulo São Paulo SP Brazil
Department of Bioengineering University of California Los Angeles California USA
Department of Biomedical Molecular Biology Ghent University Ghent Belgium
Department of Cell Biology Albert Einstein College of Medicine Bronx NY USA
Department of Chemical and Materials Engineering New Mexico State University Las Cruces NM USA
Department of Chemistry University of Tokyo Tokyo Japan
Department of Clinical and Experimental Medine Linköping University Linköping Sweden
Department of Experimental and Clinical Medicine University of Florence Florence Italy
Department of Hematology Oncology and Rheumatology University Heidelberg Heidelberg Germany
Department of Hematology Singapore General Hospital Singapore
Department of Immunobiology School of Immunology and Microbial Sciences King's College London UK
Department of Immunology and Pathology Monash University Melbourne Victoria Australia
Department of Immunology Graduate School of Medicine Chiba University Chiba city Chiba Japan
Department of Immunology IRCCS Bambino Gesu Children's Hospital Rome Italy
Department of Immunology Rady Faculty of Health Sciences University of Manitoba Winnipeg MB Canada
Department of Immunology University L Eotvos Budapest Hungary
Department of Immunology University of Toronto Toronto ON Canada
Department of Immunology University of Udine Udine Italy
Department of Infectious Disease Imperial College London UK
Department of Internal Medicine 3 University Hospital Regensburg Germany
Department of Internal Medicine Groene Hart Hospital Gouda The Netherlands
Department of Life Sciences University of Modena and Reggio Emilia Modena Italy
Department of Life Sciences University of Siena Siena Italy
Department of Mecicine University of Massachusetts Medical School Worcester MA USA
Department of Medical Biotechnologies and Translational Medicine University of Milan Milan Italy
Department of Medical Genetics University of British Columbia Vancouver BC Canada
Department of Medical Microbiology and Immunology University of Alberta Alberta Canada
Department of Medicine Albert Einstein College of Medicine Bronx NY USA
Department of Medicine Cancer Research Centre Salamanca Spain
Department of Medicine The University of British Columbia Vancouver Canada
Department of Microbial Infection and Immunity Ohio State University Columbus OH USA
Department of Microbiology and Immunology University of Gothenburg Gothenburg Sweden
Department of Molecular Medicine Sapienza University of Rome IRCCS Neuromed Pozzilli Italy
Department of Neurology Technical University of Munich Munich Germany
Department of Neuropathology Universitätsklinikum Erlangen Germany
Department of Oncology University of Lausanne and CHUV Epalinges Switzerland
Department of Parasitology Leiden University Medical Center Leiden The Netherlands
Department of Pathology and Rogel Cancer Center the University of Michigan Ann Arbor Michigan USA
Department of Pathology University of Cambridge Cambridge UK
Department of Physiopathology and Transplants University of Milan Milan Italy
Department of Precision Instruments Tsinghua University Beijing China
Department of Respiratory Sciences University of Leicester Leicester UK
Department of Rheumatology and Clinical Immunology Berlin Institute of Health Berlin Germany
Department of Rheumatology Erasmus MC University Medical Center Rotterdam Rotterdam The Netherlands
Department of Rheumatology Leiden University Medical Center Leiden The Netherlands
Department of Surgery The University of British Columbia Vancouver Canada
Dept Medicine Rheumatology and Clinical Immunology Charité Universitätsmedizin Berlin Germany
Dept Molecular Translational Medicine University of Brescia Brescia Italy
Dept of Hematopathology The University of Texas MD Anderson Cancer Center Houston TX USA
Deutsches Rheuma Forschungszentrum an Institute of the Leibniz Association Berlin Germany
Dipartimento di Medicina Interna e Specialità Mediche Sapienza Università di Roma Rome Italy
Discipline of Dermatology University of Sydney Sydney New South Wales Australia
Division of Medical Sciences National Cancer Centre Singapore Singapore
Division of Oncology Washington University School of Medicine St Louis MO USA
Divsion of Medical Oncology and Hematology Princess Margaret Hospital Toronto Ontario Canada
e Duve Institute Université catholique de Louvain Brussels Belgium
ETH Zurich Institute of Microbiology Zurich Switzerland
Executive Offices National Cancer Centre Singapore Singapore
Experimental Immunology Helmholtz Centre for Infection Research Braunschweig Germany
Experimental Medicine Division Nuffield Department of Medicine University of Oxford Oxford UK
FACS and Imaging Core Facility Max Planck Institute for Biology of Ageing Cologne Germany
Faculty of Biological Sciences Friedrich Schiller University Jena Jena Germany
Flow and Image Cytometry Shared Resource Roswell Park Comprehensive Cancer Center Buffalo NY USA
Flow Cytometry Core Facility Blizard Institute Queen Mary London University London UK
Flow Cytometry Core Facility European Molecular Biology Laboratory Heidelberg Germany
Flow Cytometry Core Facility German Cancer Research Centre Heidelberg Germany
Flow Cytometry Core Facility Institute of Experimental Immunology University of Bonn Bonn Germany
Flow Cytometry Core Humanitas Clinical and Research Center Milan Italy
Flow Cytometry Core Technologies UCD Conway Institute University College Dublin Dublin Ireland
Flow Cytometry Facility Babraham Institute Cambridge UK
Flow Cytometry Houston Methodist Hospital Research Institute Houston TX USA
Flow Cytometry Scientific Technology Platform The Francis Crick Institute London UK
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico University of Milan
Fred Hutchinson Cancer Research Center Vaccine and Infectious Disease Division Seattle WA USA
Fundação Oswaldo Cruz Minas Laboratory of Immunopatology Belo Horizonte MG Brazil
Genome Biology Unit European Molecular Biology Laboratory Heidelberg Germany
Genomics Core Facility European Molecular Biology Laboratory Heidelberg Germany
German Cancer Consortium partner site Berlin Berlin Germany
German Cancer Research Center Division of Theoretical Systems Biology Heidelberg Germany
German Cancer Research Center Heidelberg Germany
German Center for Infection Research Munich Germany
Heidelberg Institute for Stem Cell Technology and Experimental Medicine Heidelberg Germany
Heidelberg University Institute of Immunology Section of Molecular Immunology Heidelberg Germany
Honorary Dean of Life Sciences Henan University Kaifeng China
IMIM University Pompeu Fabra Barcelona Spain
Immunobiology Laboratory The Francis Crick Institute London UK
Immunology and Environment LIMES Institute University of Bonn Bonn Germany
Immunology Section Lund University Lund Sweden
Inflammation and Oncology Research Amgen Inc South San Francisco USA
INGM Fondazione Istituto Nazionale di Genetica Molecolare Ronmeo ed Enrica Invernizzi Milan Italy
Innate Immunty Unit Department of Immunology Institut Pasteur Paris France
INSERM U932 PSL University Institut Curie Paris France
Inst f Med Mikrobiology and Hospital Hygiene University of Marburg Germany
Institut für Genetik Universität zu Köln Köln Germany
Institut für Immunologie Christian Albrechts Universität zu Kiel Kiel Germany
Institut für Klinische Molekularbiologie Christian Albrechts Universität zu Kiel Germany
Institut für Transfusionsmedizin Universitätsklinik Schleswig Holstein Kiel Germany
Institut Pasteur Cellular Lymphocytes Biology Immunology Departement Paris France
Institut Pasteur Inserm U1223 Paris France
Institute for Advanced Study Shenzhen University Shenzhen China
Institute for Medical Informatics Statistics and Epidemiology University of Leipzig Leipzig Germany
Institute for Molecular Medicine Goethe University Frankfurt Frankfurt am Main Germany
Institute for Systemic Inflammation Research University of Luebeck Luebeck Germany
Institute of Bioinnovation BSRC Alexander Fleming Vari Greece
Institute of Biomedicine and Translational Medicine University of Tartu Tartu Estonia
Institute of Experimental Immunology University of Bonn Germany
Institute of Experimental Immunology University of Zurich Zurich Switzerland
Institute of Immunobiology Kantonsspital St Gallen St Gallen Switzerland
Institute of Immunology Department of Pathobiology University of Veterinary Medicine Vienna Austria
Institute of Immunology Hannover Medical School Hannover Germany
Institute of Molecular and Clinical Immunology Otto von Guericke University Magdeburg Germany
Institute of Molecular Health Sciences ETH Zurich Zürich Switzerland
Institute of Technological Sciences Wuhan University Wuhan China
Institute of Transplant Immunology Hannover Medical School MHH Hannover Germany
Institute of Virology Technical University of Munich Munich Germany
Internal Medicine 1 University Hospital Regensburg Germany
IRCCS Fondazione Don Carlo Gnocchi Milan Italy
IRCCS Fondazione Santa Lucia Rome Italy
IRCCS Istituto Giannina Gaslini Genova Italy
Isaac and Laura Perlmutter Cancer Center NYU Langone Medical Center New York USA
Istituto Clinico Humanitas IRCCS and Humanitas University Pieve Emanuele Milan Italy
Istituto Pasteur Fondazione Cenci Bolognetti Rome Italy
Jena University Hospital Institute of Immunology Jena Germany
John van Geest Cancer Research Centre Nottingham Trent University Nottingham UK
Karolinska Institutet Biomedicum Solna Sweden
Kennedy Institute of Rheumatology University of Oxford Oxford UK
King's College London Peter Gorer Department of Immunobiology London UK
Laboratory of Cytomics Joint Research Unit CIPF UVEG Principe Felipe Research Center Valencia Spain
Laboratory of Translational Immunology Humanitas Clinical and Research Center Rozzano Italy
Leibniz Research Institute for Environmental Medicine Düsseldorf Germany
Leiden Computational Biology Center Leiden University Medical Center Leiden The Netherlands
Luxembourg Centre for Systems Biomedicine University of Luxembourg Belvaux Luxembourg
Macrophage Biology Group School of Biology University of Barcelona Barcelona Spain
Max Planck Institute for Infection Biology Berlin Germany
Medinfomatics Ltd West Drayton Middlesex UK
Milan Center for Neuroscience University of Milano Bicocca Milan Italy
Murdoch Children's Research Institute Parkville Victoria Australia
Nanobiophotonics Department Leibniz Institute of Photonic Technology Jena Germany
National Institute for Health Migration and Poverty Rome Italy
National Institutes of Health Bethesda MD USA
National Key Laboratory of Medical Immunology Nankai University Tianjin China
Networked Biomedical Research Center for Hepatic and Digestive Diseases Madrid Spain
Neuroimmunology and Flow Cytometry Units Fondazione Santa Lucia IRCCS Rome Italy
Owl Biomedical Inc Santa Barbara USA
Preparative Flow Cytometry Max Delbrück Centrum für Molekulare Medizin Berlin Germany
Program in Emerging Infectious Disease Duke NUS Medical School Singapore
Purdue University Cytometry Laboratories Purdue University West Lafayette IN USA
Quantitative Systems Biology Life and Medical Sciences Institute University of Bonn Bonn Germany
R and D Reagents Miltenyi Biotec GmbH Bergisch Gladbach Germany
Regeneration in Hematopoiesis Leibniz Institute on Aging Fritz Lipmann Institute Jena Germany
Regensburg Center for Interventional Immunology Regensburg Germany
RIKEN Center for Integrative Medical Sciences Yokohama Japan
Roswell Park Comprehensive Cancer Center Elm and Carlton Streets Buffalo NY USA
School of Biological Sciences Nanyang Technological University Singapore
School of Biomedical and Health Sciences RMIT University Bundoora Victoria Australia
School of Biomedical Engineering The University of British Columbia Vancouver Canada
School of Computing Science Simon Fraser University Burnaby Canada
School of Plant Sciences and Bio5 Institute University of Arizona Tucson USA
Section of Immunology Vetsuisse Faculty University of Zurich Zurich Switzerland
Singapore Immunology Network Biopolis Singapore
Swiss Institute of Allergy and Asthma Research University of Zurich Davos Switzerland
Terry Fox Laboratory BC Cancer Vancouver BC Canada
The FIRC Institute of Molecular Oncology Milan Italy
Theodor Kocher Institute University of Bern Bern Switzerland
Thermo Fisher Scientific Eugene Oregon USA
Translational Immunology Institute SingHealth Duke NUS Academic Medical Centre Singapore
TranslaTUM Technical University of Munich Munich Germany
TRI Flow Cytometry Suite Translational Research Institute Wooloongabba QLD Australia
Tuberculosis Laboratory The Francis Crick Institute London UK
Unit Lymphopoiesis Department of Immunology Institut Pasteur Paris France
Université Paris Descartes Institut National de la Santé et de la Recherche Médicale Paris France
University Bonn Medical Faculty Bonn Germany
University of Auckland School of Biological Sciences Maurice Wilkins Center Auckland New Zealand
University of Birmingham Birmingham UK
University of Cambridge Cambridge Institute for Medical Research Cambridge UK
University of Verona Department of Medicine Section of General Pathology Verona Italy
VIB Flow Core VIB Center for Inflammation Research Ghent Belgium
William Harvey Research Institute Queen Mary University London United Kingdom
WPI Immunology Frontier Research Center Osaka University Osaka Japan
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RNA-Seq of Single Fish Cells - Seeking Out the Leukocytes Mediating Immunity in Teleost Fishes
