Cytogenetics of Eudiplozoon nipponicum (Monogenea, Diplozoidae): Karyotype, spermatocyte division and 18S rDNA location
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
31770588
DOI
10.1016/j.parint.2019.102031
PII: S1383-5769(19)30382-4
Knihovny.cz E-zdroje
- Klíčová slova
- Chromosomes, Evolution, Fluorescent in situ hybridization, Heterochromatin, Meiosis,
- MeSH
- barvení a značení MeSH
- buněčné dělení * MeSH
- cytogenetické vyšetření MeSH
- fylogeneze MeSH
- hybridizace in situ fluorescenční MeSH
- kapři parazitologie MeSH
- karyotyp MeSH
- RNA ribozomální 18S genetika MeSH
- spermatocyty fyziologie MeSH
- Trematoda genetika MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- RNA ribozomální 18S MeSH
Ectoparasitic monogeneans of the family Diplozoidae have direct and monoxenous life cycle. The cytogenetics of monogeneans in general and diplozoids in particular, is a relatively underexplored area. This is why each new detailed description of a karyotype provides significant information about the evolution of monogenean chromosomes and contributes to a better understanding of phylogenetic relationships within this group. This study offers new data on the chromosomes of Eudiplozoon nipponicum, an invasive parasite of the common carp. This species' karyotype consists of seven pairs of telocentric chromosomes (2n = 14 t). After DAPI staining, we marked heterochromatin blocks on all chromosomes in the pericentromeric region. Silver staining (AgNO3) and staining with fluorescent dye YOYO-1 revealed the presence of one large active nucleolus. Fluorescent in situ hybridization with an 18S rDNA probe revealed one cluster of ribosomal genes at the terminal part of the long arms of chromosome pair No. 7. We compared our results with studies on the phylogenetic relationships of diplozoids which applied a combination of molecular methods and classical morphological characterization and found that the results of our cytogenetic analysis are consistent with the hypothesis that E. nipponicum is more basal member of the family Diplozoidae.
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