WALTER: an easy way to online evaluate telomere lengths from terminal restriction fragment analysis
Jazyk angličtina Země Velká Británie, Anglie Médium electronic
Typ dokumentu časopisecké články
Grantová podpora
18-07027S
Grantová Agentura České Republiky
CZ.02.1.01/0.0 /0.0/15_003/0000477
European Regional and Development Fund
PubMed
33752601
PubMed Central
PMC7986547
DOI
10.1186/s12859-021-04064-0
PII: 10.1186/s12859-021-04064-0
Knihovny.cz E-zdroje
- Klíčová slova
- Online toolset, Telomere length, Terminal restriction fragments,
- MeSH
- software * MeSH
- telomery * genetika MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: Telomeres, nucleoprotein structures comprising short tandem repeats and delimiting the ends of linear eukaryotic chromosomes, play an important role in the maintenance of genome stability. Therefore, the determination of the length of telomeres is of high importance for many studies. Over the last years, new methods for the analysis of the length of telomeres have been developed, including those based on PCR or analysis of NGS data. Despite that, terminal restriction fragment (TRF) method remains the gold standard to this day. However, this method lacks universally accepted and precise tool capable to analyse and statistically evaluate TRF results. RESULTS: To standardize the processing of TRF results, we have developed WALTER, an online toolset allowing rapid, reproducible, and user-friendly analysis including statistical evaluation of the data. Given its web-based nature, it provides an easily accessible way to analyse TRF data without any need to install additional software. CONCLUSIONS: WALTER represents a major upgrade from currently available tools for the image processing of TRF scans. This toolset enables a rapid, highly reproducible, and user-friendly evaluation of almost any TRF scan including in-house statistical evaluation of the data. WALTER platform together with user manual describing the evaluation of TRF scans in detail and presenting tips and troubleshooting, as well as test data to demo the software are available at https://www.ceitec.eu/chromatin-molecular-complexes-jiri-fajkus/rg51/tab?tabId=125#WALTER and the source code at https://github.com/mlyc93/WALTER .
Faculty of Informatics Masaryk University 602 00 Brno Czech Republic
Institute of Biophysics Academy of Sciences of the Czech Republic v v i 612 00 Brno Czech Republic
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