At some stage, laying hen farming is likely to be subjected to sub-optimum temperatures (SOTem) due to climate change. While egg enrichment with n-3 fatty acids is a common practice in the poultry industry, in SOTem it has been less investigated. This study tested the effects of egg enrichment through extruded flaxseed (FLX) (180 g/kg) alone or along with hulled-soaked barley (H-SB) (170 g/kg), namely FLBA, with and without a cold-temperament additive mix (CTA) (25 g/kg: 5 g/kg flixweed (Descurainia sophia), 10 g/kg dried herb-extraction residues from pussy willow (Salix aegyptiaca) and 10 g/kg dried lemon (Citrus limon) residue) in two temperatures (20°C and SOTem: 27°C) on egg performance, yolk fatty acids, lipid components, blood biochemistry, serological enzymes, antioxidant and immune system of Hy-Line W-36 53-week-old for 9 weeks. Two hundred seventy layers were randomly distributed to nine treatments, resulting in six replicates with five hens. Hens fed flaxseed diets, regardless of temperature or CTA, had higher levels of n-3 fatty acids in yolks than others. Temperature negatively influenced feed intake and egg production. FLX + H27 and FLBA + H27 groups outperformed the other groups regarding cholesterol in yolk (10.1, 10.3 mg/g yolk), high-density lipoprotein (HDL) (5.19, 4.93 mg/g yolk), total protein (FLX + H27: 6.82 mg/dl), HDL in the blood (FLBA + H27:83.8 mg/dl), superoxide dismutase (FLBA + H27:90.4 U/ml), glutathione reductase (FLBA + H27: 1042.1 U/ml), glutathione peroxidase (FLX + H27: 1149.7 U/ml) and catalase (FLBA + H27: 12.5 U/ml). Total antibody, immunoglobulin G and immunoglobulin M after 42 days were significantly higher in chicks fed CTA-added diets (p < 0.05). Replacing corn and soya bean meal with H-SB did not negatively change the above-mentioned factors. Our findings collectively suggest that egg enrichment with n-3 fatty acids through dietary FLBA + H27 (180 g/kg flaxseed, 170 g/kg H-SB, and 25 g/kg CTA) in SOtem is suggested without any adverse impacts.

CSIRO Agriculture and Food Livestock and Aquaculture Program Bribie Island Research Centre Bribie Island Qld Australia

Department of Microbiology Pathobiology and Basic Sciences Faculty of Veterinary Medicine Razi University Kermanshah Iran

Institute for Marine and Antarctic Studies University of Tasmania Hobart Tas Australia

Institute of Aquaculture and Protection of Waters University of South Bohemia České Budějovice Czech Republic

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Abd El-Hack, M. E., Abdel-Moneim, A.-M. E., Mesalam, N. M., Mahrose, K. M., Khafaga, A. F., Taha, A. E., & Swelum, A. A. (2021). Nigella sativa seeds and its derivatives in poultry feed. In R. Mohamed Fawzy (eds.), Black cumin (Nigella sativa) seeds: Chemistry, technology, functionality, and applications (pp. 265-296). Springer. https://doi.org/10.1007/978-3-030-48798-0_18

Abd El-Hack, M. E., Alagawany, M., Shaheen, H., Samak, D., Othman, S. I., Allam, A. A., Taha, A. E., Khafaga, A. F., Arif, M., Osman, A., El Sheikh, A. I., Elnesr, S. S., & Sitohy, M. (2020). Ginger and its derivatives as promising alternatives to antibiotics in poultry feed. Animals, 10(3), 452. https://doi.org/10.3390/ani10030452

Abd El-Hack, M. E., Mahrose, K., Askar, A. A., Alagawany, M., Arif, M., Saeed, M., Abbasi, F., Soomro, R. N., Siyal, F. A., & Chaudhry, M. T. (2017). Single and combined impacts of vitamin A and selenium in diet on productive performance, egg quality, and some blood parameters of laying hens during hot season. Biological Trace Element Research, 177(1), 169-179. https://doi.org/10.1007/s12011-016-0862-5

Abdel-Moneim, A.-M., Shehata, A. M., Alzahrani, S. O., Shafi, M. E., Mesalam, N. M., Taha, A. E., Swelum, A. A., Arif, M., Fayyaz, M., & Abd El-Hack, M. E. (2020). The role of polyphenols in poultry nutrition. Journal of Animal Physiology and Animal Nutrition, 104(6), 1851-1866. https://doi.org/10.1111/jpn.13455

Abou-Elkhair, R., Abdo Basha, H., Slouma Hamouda Abd El Naby, W., Ajarem, J. S., Maodaa, S. N., Allam, A. A., & Naiel, M. A. E. (2020). Effect of a diet supplemented with the Moringa oleifera seed powder on the performance, egg quality, and Gene expression in Japanese laying quail under heat-stress. Animals, 10(5), 809. https://doi.org/10.3390/ani10050809

Ahmad, M. U. (2017). Fatty acids: Chemistry, synthesis, and applications. Elsevier.

Ahmad, S., Haq, A.-U., Yousaf, M., Kamran, Z., Rehman, A.-U., & U. Sohail, M. (2014). Production of n-3PUFA enriched eggs by feeding various dietary ratios of n-6 to n-3 fatty acids and vitamin A levels to the laying hens in hot climate. The Journal of Poultry Science, 51(2), 213-219. https://doi.org/10.2141/jpsa.0120169

Akbarian, A., Golian, A., Kermanshahi, H., De Smet, S., & Michiels, J. (2015). Antioxidant enzyme activities, plasma hormone levels and serum metabolites of finishing broiler chickens reared under high ambient temperature and fed lemon and orange peel extracts and Curcuma xanthorrhiza essential oil. Journal of Animal Physiology and Animal Nutrition, 99(1), 150-162. https://doi.org/10.1111/jpn.12188

Akbarian, A., Golian, A., Sheikh Ahmadi, A., & Moravej, H. (2011). Effects of ginger root (Zingiber officinale) on egg yolk cholesterol, antioxidant status and performance of laying hens. Journal of Applied Animal Research, 39(1), 19-21. https://doi.org/10.1080/09712119.2011.558612

Akbarian, A., Michiels, J., Degroote, J., Majdeddin, M., Golian, A., & De Smet, S. (2016). Association between heat stress and oxidative stress in poultry; mitochondrial dysfunction and dietary interventions with phytochemicals. Journal of Animal Science and Biotechnology, 7(1), 1-14. https://doi.org/10.1186/s40104-016-0097-5

Akhtari, M., Moeini, R., Mojahedi, M., & Gorji, N. (2020). Assessment the studies on the concept of Mizaj (temperament) in Persian Medicine. Journal of Complementary and Integrative Medicine, 17(3), 20180122. https://doi.org/10.1515/jcim-2018-0122

Al-Sagan, A. A., Khalil, S., Hussein, E. O., & Attia, Y. A. (2020). Effects of fennel seed powder supplementation on growth performance, carcass characteristics, meat quality, and economic efficiency of broilers under thermoneutral and chronic heat stress conditions. Animals, 10(2), 206. https://doi.org/10.3390/ani10020206.

Asadi, M., Kenari, A. A., & Esmaeili, M. (2021). Restricted-protein feeding strategy decreased the protein consumption without impairing growth performance, flesh quality and non-specific immune parameters in rainbow trout (Oncorhynchus mykiss). Aquaculture, 531, 735946. https://doi.org/10.1016/j.aquaculture.2020.735946

Attia, Y. A., Abd El-Hamid, A.-H., Abedalla, A. A., Berika, M. A., Al-Harthi, M. A., Kucuk, O., Sahin, K., & Abou-Shehema, B. M. (2016). Laying performance, digestibility and plasma hormones in laying hens exposed to chronic heat stress as affected by betaine, vitamin C, and/or vitamin E supplementation. SpringerPlus, 5(1), 1-12. https://doi.org/10.1186/s40064-016-3304-0

Attia, Y. A., Al-Harthi, M. A., El-Shafey, A. S., Rehab, Y. A., & Kim, W. K. (2016). Enhancing tolerance of broiler chickens to heat stress by supplementation with vitamin E, vitamin C and/or probiotics. Annals of Animal Science, 17(4), 1155-1169. https://doi.org/10.1515/aoas-2017-0012

Attia, Y. A., Al-Harthi, M. A., & Hassan, S. S. (2018). Turmeric (Curcuma longa Linn.) as a phytogenic growth promoter alternative for antibiotic and comparable to mannan oligosaccharides for broiler chicks. Revista Mexicana De Ciencias Pecuarias, 8(1), 11-21. https://doi.org/10.22319/rmcp.v8i1.4309

Attia, Y., El-Hamid, A., Abdallah, A., Berikaa, M., El-Gandy, M., Sahin, K., & Abou-Shehema, B. (2018). Effect of betaine, vitamin C and vitamin E on egg quality, hatchability, and markers of liver and renal functions in dual-purpose breeding hens exposed to chronic heat stress. European Poultry Science, 82.2018.

Azzam, M. M., Alhotan, R., Al-Abdullatif, A., Al-Mufarrej, S., Mabkhot, M., Alhidary, I. A., & Zheng, C. (2019). Threonine requirements in dietary low crude protein for laying hens under high-temperature environmental climate. Animals, 9(9), 586. https://doi.org/10.3390/ani9090586

Bilen, S., Altief, T. A. S., Özdemir, K. Y., Salem, M. O. A., Terzi, E., & Güney, K. (2020). Effect of lemon balm (Melissa officinalis) extract on growth performance, digestive and antioxidant enzyme activities, and immune responses in rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 46(1), 471-481. https://doi.org/10.1007/s10695-019-00737-z

Bishov, S., Masuoka, Y., & Kapsalis, J. (1977). Antioxidant effect of spices, herbs and protein hydrolyzates in freeze-dried model systems: Synergistic action with synthetic phenolic antioxidants. Journal of Food Processing and Preservation, 1(2), 153-166. https://doi.org/10.1111/j.1745-4549.1977.tb00320.x

Brewer, M. (2011). Natural antioxidants: Sources, compounds, mechanisms of action, and potential applications. Comprehensive Reviews in Food Science and Food Safety, 10(4), 221-247. https://doi.org/10.1111/j.1541-4337.2011.00156.x

Büyükkılıç Beyzi, S., Konca, Y., Kaliber, M., Sarıözkan, S., Kocaoğlu Güçlü, B., Aktuğ, E., & Şentürk, M. (2020). Effects of thyme essential oil and A, C, and E vitamin combinations to diets on performance, egg quality, MDA, and 8-OHdG of laying hens under heat stress. Journal of Applied Animal Research, 48(1), 126-132. https://doi.org/10.1080/09712119.2020.1746662

Cai, X., Zhang, L. U., Chen, X., Zhang, H., Xue, H., Lu, Y., Tang, J., & Lu, Y. (2020). Orthosiphon stamineus and rosmarinic acid reduce heat stress in laying hens. Livestock Science, 240, 104124. https://doi.org/10.1016/j.livsci.2020.104124

Corrales-Retana, L., Ciucci, F., Conte, G., Casarosa, L., Mele, M., & Serra, A. (2020). Profile of fatty acid lipid fractions of omega-3 fatty acid-enriched table eggs. Journal of Animal Physiology and Animal Nutrition, 105(2), 326-335. https://doi.org/10.1111/jpn.13462

Diaz, G., Squires, E., & Julian, R. (1999). The use of selected plasma enzyme activities for the diagnosis of fatty liver-hemorrhagic syndrome in laying hens. Avian Diseases, 43(4), 768-773. https://doi.org/10.2307/1592746

Elkin, R. G., & Rogler, J. C. (1990). Reduction of the cholesterol content of eggs by the oral administration of lovastatin to laying hens. Journal of Agricultural and Food Chemistry, 38(8), 1635-1641. https://doi.org/10.1021/jf00098a003

Emami, N. K., Jung, U., Voy, B., & Dridi, S. (2021). Radical response: Effects of heat stress-induced oxidative stress on lipid metabolism in the avian liver. Antioxidants, 10(1), 35. https://doi.org/10.3390/antiox10010035

Esmaeili, M., Abedian Kenari, A., & Rombenso, A. (2017a). Effects of fish meal replacement with meat and bone meal using garlic (Allium sativum) powder on growth, feeding, digestive enzymes and apparent digestibility of nutrients and fatty acids in juvenile rainbow trout (Oncorhynchus mykiss Walbaum, 1792). Aquaculture Nutrition, 23(6), 1225-1234. https://doi.org/10.1111/anu.12491

Esmaeili, M., Abedian Kenari, A., & Rombenso, A. (2017b). Immunohematological status under acute ammonia stress of juvenile rainbow trout (Oncorhynchus mykiss Walbaum, 1792) fed garlic (Allium sativum) powder-supplemented meat and bone meal-based feeds. Comparative Clinical Pathology, 26(4), 853-866. https://doi.org/10.1007/s00580-017-2457-8

Farag, M. R., & Alagawany, M. (2018). Physiological alterations of poultry to the high environmental temperature. Journal of Thermal Biology, 76, 101-106. https://doi.org/10.1016/j.jtherbio.2018.07.012

Gado, A. R., Ellakany, H. F., Elbestawy, A. R., Abd El-Hack, M. E., Khafaga, A. F., Taha, A. E., Arif, M., & Mahgoub, S. A. (2019). Herbal medicine additives as powerful agents to control and prevent avian influenza virus in poultry - A review. Annals of Animal Science, 19(4), 905-935. https://doi.org/10.2478/aoas-2019-0043

Gholami, M., Chamani, M., Seidavi, A., Sadeghi, A. A., & Aminafschar, M. (2020). Effects of stocking density and environmental conditions on performance, immunity, carcase characteristics, blood constitutes, and economical parameters of cobb 500 strain broiler chickens. Italian Journal of Animal Science, 19(1), 524-535. https://doi.org/10.1080/1828051X.2020.1757522

Ghosi Mobaraki, M. R., Abedian Kenari, A., Bahrami Gorji, S., & Esmaeili, M. (2020). Effect of dietary fish and vegetable oil on the growth performance, body composition, fatty acids profile, reproductive performance and larval resistance in pearl gourami (Trichogaster leeri). Aquaculture Nutrition, 26(3), 894-907. https://doi.org/10.1111/anu.13048

Gill, A. (2020). Enrichment of essential fatty acids in food. In Essential fatty acids (pp. 157-182). CRC Press. https://doi.org/10.1201/9780429321115-9

Giraldo, P., Benavente, E., Manzano-Agugliaro, F., & Gimenez, E. (2019). Worldwide research trends on wheat and barley: A bibliometric comparative analysis. Agronomy, 9:352. https://doi.org/10.3390/agronomy9070352.

Gous, R., & Morris, T. (2005). Nutritional interventions in alleviating the effects of high temperatures in broiler production. World’s Poultry Science Journal, 61(3), 463-475. https://doi.org/10.1079/WPS200568

Hashemi, S., & Davoodi, H. (2012). Herbal plants as new immuno-stimulator in poultry industry: A review. Asian Journal of Animal and Veterinary Advances, 7(2), 105-116. https://doi.org/10.3923/ajava.2012.105.116

Haugh, R. (1937). The Haugh unit for measuring egg quality. United States Egg Poultry, 43(522-555), 572-573.

He, S., Arowolo, M., Medrano, R., Li, S., Yu, Q., Chen, J., & He, J. (2018). Impact of heat stress and nutritional interventions on poultry production. World's Poultry Science Journal, 74(4), 647-664. https://doi.org/10.1017/S0043933918000727

Hosseini, H., Esmaeili, M., Fahim, A., Sepehr, A., & Rombenso, A. (2021). Herbal medicine alleviates the adverse effects of high inclusion of extruded flaxseed (Linum usitatissimum) on antioxidant and immune systems of laying hen: A comprehensive study. Journal of Animal Physiology and Animal Nutrition, under Review.

Huang, S., Baurhoo, B., & Mustafa, A. (2020). Effects of feeding extruded flaxseed on layer performance, total tract nutrient digestibility, and fatty acid concentrations of egg yolk, plasma and liver. Journal of Animal Physiology and Animal Nutrition, 104(5), 1365-1374. https://doi.org/10.1111/jpn.13364

Ibtisham, F., Nawab, A., Niu, Y., Wang, Z., Wu, J., Xiao, M., & An, L. (2019). The effect of ginger powder and Chinese herbal medicine on production performance, serum metabolites and antioxidant status of laying hens under heat-stress condition. Journal of Thermal Biology, 81, 20-24. https://doi.org/10.1016/j.jtherbio.2019.02.002

Ighodaro, O., & Akinloye, O. (2018). First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine, 54(4), 287-293. https://doi.org/10.1016/j.ajme.2017.09.001

Ilkhani, R., Aghanouri, Z., Mojahedi, M., Montazeri, A., Siavash, M., & Tabatabaei, F. (2019). Comparing Mizaj (temperament) in type 1 diabetes mellitus and healthy controls: A case-control study. Journal of Research in Medical Sciences, 24, 58. https://doi.org/10.4103/jrms.JRMS_980_18

Jia, W., Slominski, B., Guenter, W., Humphreys, A., & Jones, O. (2008). The effect of enzyme supplementation on egg production parameters and omega-3 fatty acid deposition in laying hens fed flaxseed and canola seed. Poultry Science, 87(10), 2005-2014. https://doi.org/10.3382/ps.2007-00474

Jiang, Z., Ahn, D. U., & Sim, J. S. (1991). Effects of feeding flax and two types of sunflower seeds on fatty acid compositions of yolk lipid classes. Poultry Science, 70(12), 2467-2475. https://doi.org/10.3382/ps.0702467

Johannson, S., Raginski, C., Schwean-Lardner, K., & Classen, H. (2016). Providing laying hens in group-housed enriched cages with access to barley silage reduces aggressive and feather-pecking behaviour. Canadian Journal of Animal Science, 96(2), 161-171. https://doi.org/10.1139/cjas-2015-0133

Kim, D.-H., Lee, Y.-K., Kim, S.-H., & Lee, K.-W. (2021). The impact of temperature and humidity on the performance and physiology of laying hens. Animals, 11(1), 56. https://doi.org/10.3390/ani11010056

Kim, D.-H., Lee, Y.-K., Lee, S.-D., Kim, S.-H., Lee, S.-R., Lee, H.-G., & Lee, K.-W. (2020). Changes in production parameters, egg qualities, fecal volatile fatty acids, nutrient digestibility, and plasma parameters in laying hens exposed to ambient temperature. Frontiers in Veterinary Science, 7, 412. https://doi.org/10.3389/fvets.2020.00412

Kim, J. H., Lee, H. K., Yang, T. S., Kang, H. K., & Kil, D. Y. (2019). Effect of different sources and inclusion levels of dietary fat on productive performance and egg quality in laying hens raised under hot environmental conditions. Asian-Australasian Journal of Animal Sciences, 32(9), 1407. https://doi.org/10.5713/ajas.19.0063

Kop-Bozbay, C., Akdag, A., Atan, H., & Ocak, N. (2020). Response of broilers to supplementation of branched-chain amino acids blends with different valine contents in the starter period under summer conditions. Animal Bioscience, 34(2), 295-305. https://doi.org/10.5713/ajas.19.0828

Kothari, D., Lee, W.-D., Niu, K.-M., & Kim, S.-K. (2019). The genus Allium as poultry feed additive: A review. Animals, 9(12), 1032. https://doi.org/10.3390/ani9121032

Kumari, P., Chandramoni, Singh, P. K., & Singh, A. K. (2020). Effect of different herbs and spices on cholesterol lowering in broiler: An overview. Indian Journal of Poultry Science, 55(2), 91-97. https://doi.org/10.5958/0974-8180.2020.00020.3

Lara, L. J., & Rostagno, M. H. (2013). Impact of heat stress on poultry production. Animals, 3(2), 356-369. https://doi.org/10.3390/ani3020356

Li, X., He, W., Wang, Z., & Xu, T. (2016). Effects of Chinese herbal mixture on performance, egg quality and blood biochemical parameters of laying hens. Journal of Animal Physiology and Animal Nutrition, 100(6), 1041-1049. https://doi.org/10.1111/jpn.12473

Liu, M., Lu, Y., Gao, P., Xie, X., Li, D., Yu, D., & Yu, M. (2020). Effect of curcumin on laying performance, egg quality, endocrine hormones, and immune activity in heat-stressed hens. Poultry Science, 99(4), 2196-2202. https://doi.org/10.1016/j.psj.2019.12.001

Marques, L. R., Diniz, T. A., Antunes, B. M., Rossi, F. E., Caperuto, E. C., Lira, F. S., & Gonçalves, D. C. (2018). Reverse cholesterol transport: molecular mechanisms and the non-medical approach to enhance HDL cholesterol. Frontiers in Physiology, 9, 526. https://doi.org/10.3389/fphys.2018.00526

Mignon-Grasteau, S., Moreri, U., Narcy, A., Rousseau, X., Rodenburg, T., Tixier-Boichard, M., & Zerjal, T. (2015). Robustness to chronic heat stress in laying hens: A meta-analysis. Poultry Science, 94(4), 586-600. https://doi.org/10.3382/ps/pev028

Montazeri Parchikolaei, H., Abedian Kenari, A., & Esmaeili, M. (2021). Soybean-based diets plus probiotics improve the profile of fatty acids, digestibility, intestinal microflora, growth performance, and the innate immunity of beluga (Huso huso). Aquaculture Research, 52(1), 152-166. https://doi.org/10.1111/are.14877

Nawab, A., Tang, S., Li, G., An, L., Wu, J., Liu, W., & Xiao, M. (2020). Dietary curcumin supplementation effects on blood immunological profile and liver enzymatic activity of laying hens after exposure to high temperature conditions. Journal of Thermal Biology, 90, 102573. https://doi.org/10.1016/j.jtherbio.2020.102573

Njembe, M. N., Dormal, E., Gardin, C., Mignolet, E., Debier, C., & Larondelle, Y. (2021). Effect of the dietary combination of flaxseed and Ricinodendron heudelotii or Punica granatum seed oil on the fatty acid profile of eggs. Food Chemistry, 344, 128668. https://doi.org/10.1016/j.foodchem.2020.128668

Nogueira, M. S., Scolaro, B., Milne, G. L., & Castro, I. A. (2019). Oxidation products from omega-3 and omega-6 fatty acids during a simulated shelf life of edible oils. LWT, 101, 113-122. https://doi.org/10.1016/j.lwt.2018.11.044

Orhan, C., Tuzcu, M., Deeh, P. B. D., Sahin, N., Komorowski, J. R., & Sahin, K. (2019). Organic chromium form alleviates the detrimental effects of heat stress on nutrient digestibility and nutrient transporters in laying hens. Biological Trace Element Research, 189(2), 529-537. https://doi.org/10.1007/s12011-018-1485-9

Panaite, T. D., Turcu, R. P., Soica, C., & Visinescu, P. (2020). Nutritional parameters of eggs from laying hens fed with flaxseed meal or mixture with rapeseed meal or rice bran. Journal of Applied Animal Research, 48(1), 566-574. https://doi.org/10.1080/09712119.2020.1848846

Parvinroo, S., Zahediasl, S., Sabetkasaei, M., Kamalinejad, M., & Naghibi, F. (2014). The effects of selected hot and cold temperament herbs based on Iranian traditional medicine on some metabolic parameters in normal rats. Iranian Journal of Pharmaceutical Research, 13(Suppl), 177.

Pliego, A. B., Tavakoli, M., Khusro, A., Seidavi, A., Elghandour, M. M. M. Y., Salem, A. Z. M., Márquez-Molina, O., & Rene Rivas-Caceres, R. (2020). Beneficial and adverse effects of medicinal plants as feed supplements in poultry nutrition: A review. Animal Biotechnology, 1-23. https://doi.org/10.1080/10495398.2020.1798973

Quinteiro-Filho, W. M., Ribeiro, A., Ferraz-de-Paula, V., Pinheiro, M. L., Sakai, M., Sá, L., Ferreira, A., & Palermo-Neto, J. (2010). Heat stress impairs performance parameters, induces intestinal injury, and decreases macrophage activity in broiler chickens. Poultry Science, 89(9), 1905-1914. https://doi.org/10.3382/ps.2010-00812

Rafiee, F., Mazhari, M., Ghoreishi, M., & Esmaeilipour, O. (2016). Effect of lemon verbena powder and vitamin C on performance and immunity of heat-stressed broilers. Journal of Animal Physiology and Animal Nutrition, 100(5), 807-812. https://doi.org/10.1111/jpn.12457

Ramezanzadeh, S., Abedian Kenari, A., & Esmaeili, M. (2020). Immunohematological parameters of rainbow trout (Oncorhynchus mykiss) fed supplemented diet with different forms of barberry root (Berberis vulgaris). Comparative Clinical Pathology, 29, 177-187. https://doi.org/10.1007/s00580-019-03032-8

Ramezanzadeh, S., Abedian Kenari, A., Esmaeili, M., & Rombenso, A. (2021). Effects of different forms of barberry root (Berberis vulgaris) on growth performance, muscle fatty acids profile, whole-body composition, and digestive enzymes of rainbow trout (Oncorhynchus mykiss). Journal of the World Aquaculture Society, 52, 284-302. https://doi.org/10.1111/jwas.12722

Redza-Dutordoir, M., & Averill-Bates, D. A. (2016). Activation of apoptosis signalling pathways by reactive oxygen species. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1863(12), 2977-2992. https://doi.org/10.1016/j.bbamcr.2016.09.012

Sahin, N., Hayirli, A., Orhan, C., Tuzcu, M., Akdemir, F., Komorowski, J., & Sahin, K. (2017). Effects of the supplemental chromium form on performance and oxidative stress in broilers exposed to heat stress. Poultry Science, 96(12), 4317-4324. https://doi.org/10.3382/ps/pex249

Saleh, A. A., Kirrella, A. A., Dawood, M. A., & Ebeid, T. A. (2019). Effect of dietary inclusion of cumin seed oil on the performance, egg quality, immune response and ovarian development in laying hens under high ambient temperature. Journal of Animal Physiology and Animal Nutrition, 103(6), 1810-1817. https://doi.org/10.1111/jpn.13206

Sayyari, Z., & Farahmandfar, R. (2017). Stabilization of sunflower oil with pussy willow (Salix aegyptiaca) extract and essential oil. Food Science & Nutrition, 5(2), 266-272. https://doi.org/10.1002/fsn3.389

Seidavi, A., Belali, M., Elghandour, M. M., Adegbeye, M. J., & Salem, A. (2020). Potential impacts of dietary inclusion of green tea (Camellia sinensis L.) in poultry feeding: A review. Agroforestry Systems, 94(4), 1161-1170. https://doi.org/10.1007/s10457-019-00444-x

Sepehr, A., Bahari Kashani, R., Esmaeili, M., Safari, O., & Rombenso, A. (2021). Effects of extruded, milled, and whole flaxseed (Linum usitatissimum) on egg performance, lipid components, and fatty acids concentrations in yolk and blood, and antioxidant system of commercial laying hens. Animal Feed Science and Technology, 276, 114877. https://doi.org/10.1016/j.anifeedsci.2021.114877

Shahidi, B., Sharifi, A., Nasiraie, L. R., Niakousari, M., & Ahmadi, M. (2020). Phenolic content and antioxidant activity of flixweed (Descurainia sophia) seeds extracts: Ranking extraction systems based on fuzzy logic method. Sustainable Chemistry and Pharmacy, 16, 100245. https://doi.org/10.1016/j.scp.2020.100245

Sugiharto, S., Yudiarti, T., Isroli, I., Widiastuti, E., & Kusumanti, E. (2017). Dietary supplementation of probiotics in poultry exposed to heat stress - A review. Annals of Animal Science, 17(3), 591-604. https://doi.org/10.1515/aoas-2016-0062

Surai, P., & Fisinin, V. (2015). Antioxidant-prooxidant balance in the intestine: Applications in chick placement and pig weaning. Journal of Veterinary Science & Medicine, 3(1), 66-84.

Svihus, B., Newman, R., & Newman, C. (1997). Effect of soaking, germination, and enzyme treatment of whole barley on nutritional value and digestive tract parameters of broiler chickens. British Poultry Science, 38(4), 390-396. https://doi.org/10.1080/00071669708418008

Tamiru, M., Ashagrie, S., Alkhtib, A., Getachew, M., Demeke, S., Hassen, W., Worku, Z., & Burton, E. (2020). Performance of broilers and layers supplemented with Moringa stenopetala leaf meal under hot humid tropical conditions. Animal Production Science, 60(17), 1987-1994. https://doi.org/10.1071/AN19671

Teymouri, H., Zarghi, H., & Golian, A. (2018). Evaluation of hull-less barley with or without enzyme cocktail in the finisher diets of broiler chickens. Journal of Agricultural Science and Technology, 20(3), 469-483.

Toroglu, S. (2011). In-vitro antimicrobial activity and synergistic/antagonistic effect of interactions between antibiotics and some spice essential oils. Journal of Environmental Biology, 32(1), 23-29.

Upadhaya, S., Park, J., & Kim, I. (2017). Effect of replacement of de-hulled barley with water-soaked barley in corn-soybean meal-based diet on growth performance, blood characteristics, and meat quality in finishing pigs. Journal of Applied Animal Research, 45(1), 164-169. https://doi.org/10.1080/09712119.2015.1124332

Vandana, G., Sejian, V., Lees, A., Pragna, P., Silpa, M., & Maloney, S. K. (2021). Heat stress and poultry production: Impact and amelioration. International Journal of Biometeorology, 65, 163-179. https://doi.org/10.1007/s00484-020-02023-7

Wen, C., Gu, Y., Tao, Z., Cheng, Z., Wang, T., & Zhou, Y. (2019). Effects of ginger extract on laying performance, egg quality, and antioxidant status of laying hens. Animals, 9(11), 857. https://doi.org/10.3390/ani9110857

Wijendran, V., & Hayes, K. (2004). Dietary n-6 and n-3 fatty acid balance and cardiovascular health. Annual Review of Nutrition, 24, 597-615. https://doi.org/10.1146/annurev.nutr.24.012003.132106

Xi, W., Lu, J., Qun, J., & Jiao, B. (2017). Characterization of phenolic profile and antioxidant capacity of different fruit part from lemon (Citrus limon Burm.) cultivars. Journal of Food Science and Technology, 54(5), 1108-1118. https://doi.org/10.1007/s13197-017-2544-5

Zeilab Sendijani, R., Abedian Kenari, A., Smiley, A. H., & Esmaeili, M. (2020). The effect of extract from dill (Anethum graveolens) on the growth performance, body composition, immune system and antioxidant system of Rainbow Trout (Oncorhynchus mykiss). North American Journal of Aquaculture, 82(2), 119-131. https://doi.org/10.1002/naaq.10123

The Recovery Time between Early Mild Stress and Final Acute Stress Affects Survival Rate, Immunity, Health, and Physiology of Oscar (Astronotus ocellatus)

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