A one-pot and facile method with assistance of high gravity was applied for the synthesis of inorganic two-dimensional MOF-5 embedded MXene nanostructures. The innovative inorganic MXene/MOF-5 nanostructure was applied in co-delivery of drug and gene, and to increase its bioavailability and interaction with the pCRISPR, the nanomaterial was coated with alginate and chitosan. The polymer-coated nanosystems were fully characterized, and the sustained DOX delivery and comprehensive cytotoxicity studies were conducted on the HEK-293, PC12, HepG2, and HeLa cell lines, demonstrating acceptable and excellent cell viability at both very low (0.1 μg.mL-1) and high (10 μg·mL-1) concentrations. The chitosan-coated nanocarriers showed superior relative cell viability compared to others, more than 60% on average of relative cell viability in all of the cell lines. Then, alginate-coated nanocarriers ranked at second place on the higher relative cell viability, more than 50% on average for all of the cell lines. Also, MTT results showed a complete dose-dependence, and by increasing the time of treatment from 24 to 72 h, the relative cell viability decreased by a meaningful slope; however, this decrease was optimized by coating the nanocarrier with chitosan and alginate. The nanosystems were also tagged with pCRISPR to analyze the potential application in the co-delivery of drug/gene. CLSM images of the HEK-293 and HeLa cell lines unveiled successful delivery of pCRISPR into the cells, and the enhanced green fluorescent protein (EGFP) reached up to ca. 26% for the HeLa cell line. Also, a considerable drug payload of 35.7% was achieved, which would be because of the interactions between the nanocarrier and the doxorubicin. In this unprecedented report pertaining to the synthesis of MXene assisted by a MOF and high-gravity technique, the methodology and the optimized ensuing MXene/MOF-5 nanosystems can be further developed for the co-delivery of drug/gene in animal models.

Center of Excellence in Electrochemistry School of Chemistry College of Science University of Tehran Tehran142411 Iran

Department of Chemistry Sharif University of Technology Tehran 11155 3516 Iran

Department of Materials Science and Engineering Research Institute of Advanced Materials Seoul National University Seoul 08826 Republic of Korea

Laboratoire Matériaux Optiques Photonique and Systèms Université de Lorraine CentraleSupélec F 57000 Metz France

Lunenfeld Tanenbaum Research Institute Mount Sinai Hospital University of Toronto Toronto M5S 1A1 Canada

Regional Center of Advanced Technologies and Materials Palacky University Šlechtitelů 27 783 71 Olomouc Czech Republic

School of Chemical Engineering Oklahoma State University 420 Engineering North Stillwater Oklahoma 74078 United States

Citace poskytuje Crossref.org

CaZnO-based nanoghosts for the detection of ssDNA, pCRISPR and recombinant SARS-CoV-2 spike antigen and targeted delivery of doxorubicin

Advanced MXene-Based Micro- and Nanosystems for Targeted Drug Delivery in Cancer Therapy

Quantum dots against SARS-CoV-2: diagnostic and therapeutic potentials

Porphyrin Molecules Decorated on Metal-Organic Frameworks for Multi-Functional Biomedical Applications