In this article, we present a unique combination of techniques focusing on the immobilization of noble metal nanoparticles into a honeycomb polystyrene pattern prepared with the improved phase-separation technique. The procedure consists of two main steps: the preparation of the honeycomb pattern (HCP) on a perfluoroethylenepropylene substrate (FEP), followed by an immobilization procedure realized by the honeycomb pattern's exposure to an excimer laser in a noble metal nanoparticle solution. The surface physico-chemical properties, mainly the surface morphology and chemistry, are characterized in detail in the study. The two-step procedure represents the unique architecture of the surface immobilization process, which reveals a wide range of potential applications, mainly in tissue engineering, but also as substrates for analytical use.

Department of Solid State Engineering University of Chemistry and Technology Prague Technická 5 16628 Prague Czech Republic

Faculty of Science J E Purkyne University 40001 Usti Nad Labem Czech Republic

Innovation Centre of the Institute of Macromolecular Chemistry Academy of Sciences of Czech Republic Prague 6 Brevnov 16200 Prague Czech Republic

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Biopolymer Honeycomb Microstructures: A Review