INTRODUCTION: Based on previous reports of defense-related defects in the Arabidopsis loss-of-function (LOF) mutants of the EXO70B1 and EXO70B2 exocyst subunits, we investigated the underlying causes of these phenotypes. METHODS: We analyzed stomatal aperture states in both young and adult plants. As the exocyst is involved in the secretion to the cell wall, we examined cell wall composition, and we correlated these findings via a comprehensive mRNA expression analysis. RESULTS: Our results revealed and discovered a more closed initial state of stomatal opening in exo70B mutants, as well as altered methylation and acetylation modifications of pectin and hemicellulose in the studied mutant lines. These changes in cell wall modifications may contribute to both compromised stomatal aperture-dependent and stomatal aperture-independent defense responses, as well as to the transcriptional activation of defense pathways observed in non-infected mutant plants at adult developmental stages. Several candidate genes involved in these processes were pinpointed using RNA-seq analysis. DISCUSSION: Interestingly, although the primary phenotypic and RNA-seq deviations in young mutant lines may be specific for each of the two EXO70B mutant lines, they exhibit converging gene expression profiles in later developmental stages. This convergence may reflect the shared evolutionary origin of the two Brassicaceae EXO70 isoforms by duplication from a common ancestral gene.

Department of Experimental Plant Biology Faculty of Science Charles University Prague Czechia

Laboratory of Cell Biology Institute of Experimental Botany of the Czech Academy of Sciences Prague Czechia

Laboratory of Hormonal Regulations in Plants Institute of Experimental Botany of the Czech Academy of Sciences Prague Czechia

Université Paris Saclay Institut National de Recherche pour l'Agriculture l'Alimentation et l'Environnement Versailles France

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