Cross-contamination of eukaryotic cell lines used in biomedical research represents a highly relevant problem. Analysis of repetitive DNA sequences, such as Short Tandem Repeats (STR), or Simple Sequence Repeats (SSR), is a widely accepted, simple, and commercially available technique to authenticate cell lines. However, it provides only qualitative information that depends on the extent of reference databases for interpretation. In this work, we developed and validated a rapid and routinely applicable method for evaluation of cell culture cross-contamination levels based on mass spectrometric fingerprints of intact mammalian cells coupled with artificial neural networks (ANNs). We used human embryonic stem cells (hESCs) contaminated by either mouse embryonic stem cells (mESCs) or mouse embryonic fibroblasts (MEFs) as a model. We determined the contamination level using a mass spectra database of known calibration mixtures that served as training input for an ANN. The ANN was then capable of correct quantification of the level of contamination of hESCs by mESCs or MEFs. We demonstrate that MS analysis, when linked to proper mathematical instruments, is a tangible tool for unraveling and quantifying heterogeneity in cell cultures. The analysis is applicable in routine scenarios for cell authentication and/or cell phenotyping in general.
- MeSH
- analýza hlavních komponent MeSH
- buněčné linie MeSH
- hmotnostní spektrometrie metody MeSH
- kalibrace MeSH
- kokultivační techniky MeSH
- lidé MeSH
- lidské embryonální kmenové buňky fyziologie MeSH
- multivariační analýza MeSH
- myši MeSH
- neuronové sítě (počítačové) * MeSH
- odběr biologického vzorku MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Metal-based coordination compounds have been used throughout the history of human medicine to treat various diseases, including cancer. Since the discovery of cisplatin in 1965, a great number of metal coordination complexes, such as platinum, ruthenium, gold or copper have been designed, synthesized and tested in order to develop clinically effective and safe drugs. Currently, many reviews cover applications of cytostatic metal complexes pointing out the most promising examples of platinum- and non-platinum-based compounds in preclinical and clinical trials. However, recent comprehensive reviews covering chemical and biological aspects of metal-based coordination compounds in cancer therapy are still rare. In this review we wish to provide an overview of the coordination chemistry of current and novel cytostatic compounds, including an outline of their design and rationale of synthesis, and summarize bio-chemical reactivity and physicochemical properties of candidate metal complexes.
- MeSH
- antitumorózní látky * farmakologie terapeutické užití MeSH
- cisplatina dějiny farmakologie terapeutické užití MeSH
- galium dějiny farmakologie terapeutické užití MeSH
- genomika metody trendy MeSH
- individualizovaná medicína metody trendy využití MeSH
- kobalt dějiny farmakologie terapeutické užití MeSH
- komplexní sloučeniny * farmakologie terapeutické užití MeSH
- lidé MeSH
- měď farmakologie terapeutické užití MeSH
- metabolomika metody trendy MeSH
- mezioborová komunikace MeSH
- proteomika metody trendy MeSH
- sloučeniny ruthenia dějiny farmakologie terapeutické užití MeSH
- sloučeniny železa dějiny farmakologie terapeutické užití MeSH
- sloučeniny zlata dějiny farmakologie terapeutické užití MeSH
- statistika jako téma MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
