Lactoferrin, a globular glycoprotein, is an important component of saliva. It shows an antibacterial, anticancerogenic and anti-inflammatory activity. The aim of this study was to develop a method of isolation of lactoferrin from human saliva using ion exchange chromatography in a monolithic column and spectrometric detection with Pyrogallol Red by the Bradford and biuret methods. The calibration curve for lactoferrin was linear in the range 0.0662.5 μg ml1, limit of detection 0.01 μg ml1. The lactoferin concentration in saliva of healthy subjects was 42±4 μg mg1. Patient with celiac disease showed 2.5x times higher concentration of lactoferrin compared with healthy subjects.
- MeSH
- chromatografie iontoměničová * metody přístrojové vybavení využití MeSH
- chromatografie kapalinová * metody přístrojové vybavení využití MeSH
- dospělí MeSH
- laktoferrin * analýza MeSH
- lidé MeSH
- limita detekce * MeSH
- metody pro přípravu analytických vzorků MeSH
- mladý dospělý MeSH
- sliny * MeSH
- spektrofotometrie ultrafialová MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
Deoxynivalenol (DON) is one of several mycotoxins produced by certain Fusarium species that frequently infect corn, wheat, oats, barley, rice, and other grains in the field or during storage. The exposure risk to human is directly through foods of plant origin (cereal grains) or indirectly through foods of animal origin (kidney, liver, milk, eggs). It has been detected in buckwheat, popcorn, sorgum, triticale, and other food products including flour, bread, breakfast cereals, noodles, infant foods, pancakes, malt and beer. DON affects animal and human health causing acute temporary nausea, vomiting, diarrhea, abdominal pain, headache, dizziness, and fever. This review briefly summarizes toxicities of this mycotoxin as well as effects on reproduction and their antagonistic and synergic actions.
- MeSH
- financování organizované MeSH
- Fusarium patogenita MeSH
- jedlá semena MeSH
- kontaminace potravin statistika a číselné údaje MeSH
- lidé MeSH
- mykotoxiny otrava toxicita MeSH
- toxické účinky toxicita MeSH
- trichotheceny otrava toxicita MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- přehledy MeSH
