In the field of tissue engineering, much research has been devoted to the surface topography of conductive materials. However, less work has been carried out on how the electrical stimulation of such materials influences nerve regeneration. Here, we investigated the effect of electrical stimulation on randomly- and uniaxially-aligned polypyrrole-coated cellulose acetate butyrate (PPy/CAB) nanofibers. First, SEM revealed that the conducting PPy coverage resulted in dramatic changes to the nanofiber morphology. In turn, these changes led to an increase in the sample wettability. Fourier transform spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) confirmed the presence of a PPy layer. Second, human neuroblastoma cells (SH-SY5Y) were seeded on the PPy/CAB nanofibers and stimulated by 100 mV mm-1 at 1 Hz pulses in vitro. We demonstrated that either with or without this electrical stimulation both nanofiber alignment and PPy coverage had a strong influence on cell morphology and attachment. Moreover, fluorescence microscopy revealed that the cells stimulated on PPy/CAB had longer neurite outgrowth. Collectively, our results shed light on the combined effect of scaffold morphology and external stimulation on neuronal cell behavior.
- MeSH
- buněčná adheze účinky léků MeSH
- celulosa analogy a deriváty farmakologie toxicita MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nanovlákna chemie toxicita MeSH
- neurity účinky léků MeSH
- neuronální růst účinky léků MeSH
- polymery farmakologie toxicita MeSH
- proliferace buněk účinky léků MeSH
- pyrroly farmakologie toxicita MeSH
- smáčivost MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Surface modification is an important step in making a synthetic polymer cytocompatible. We have previously reported improved cytocompatibility of immortalized human keratinocytes (HaCaT) with the otherwise bioinert fluorinated ethylene propylene (FEP) upon treatment with argon plasma discharge. In this article, we show that FEP modified with Ar plasma with the power of 3 and 8 W for 40 and 240 s served as a suitable material for cultivation of primary human dermal fibroblasts (HDF), which showed significantly improved proliferation and spreading comparable to standard tissue culture polystyrene. We also evaluated focal adhesions formed by HDF cells on modified FEP, which were far more numerous compared to pristine FEP. Moreover, we attempted spontaneous osteogenic differentiation of adipose-derived mesenchymal stem cells modified with human telomerase reverse transcriptase on Ar plasma-modified FEP. While the spontaneous osteogenic differentiation was unsuccessful, the cells were able to adhere and differentiated on tested matrices upon the administration of osteodifferentiation medium. These combined findings suggest that the treatment of FEP with Ar plasma comprises and efficient method to enable the adhesion and proliferation of various cell types on an otherwise largely bioinert material.
- MeSH
- argon chemie MeSH
- buněčná adheze MeSH
- buněčná diferenciace MeSH
- buněčné linie MeSH
- fibroblasty cytologie metabolismus MeSH
- lidé MeSH
- mezenchymální kmenové buňky cytologie metabolismus MeSH
- osteogeneze MeSH
- plazmové plyny chemie MeSH
- polytetrafluoroethylen analogy a deriváty chemie MeSH
- proliferace buněk MeSH
- škára cytologie metabolismus MeSH
- tuková tkáň cytologie metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- argon chemie MeSH
- biokompatibilní materiály chemie MeSH
- buněčná adheze MeSH
- buněčné linie MeSH
- fluorescenční barviva MeSH
- keratinocyty cytologie fyziologie MeSH
- lidé MeSH
- optické zobrazování metody MeSH
- plazmové plyny chemie MeSH
- polytetrafluoroethylen analogy a deriváty chemie MeSH
- povrchové vlastnosti MeSH
- viabilita buněk MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
