Aims: the presented study is aimed at the evaluation of correlation of free light chains serum levels – kappa, lambdaand their relation (K/L ratio) and serum levels of selected biological markers in a group of patients with multiplemyeloma examined at the time of the diagnosis. Methods: 102 patients with multiple myeloma were included in this prospective study. Free light chains serumlevels were determined by FreeliteTM Binding Site system, for determination of serum levels of selected parameters thefollowing methods were used: REA thymidinekinase (TK), RIA (ß2microglobulin (ß2m), ICTP, PINP), enzymoimmunoassay(sIL-6R, sVCAM-1, sICAM-1, sOPG) and quantitative enzymatic immunoassay (sHGF, sVEGF, sSyndecan-1(sSyn-1) a sFas). Results: There was found a correlation in the kappa-group of the dominant kappa chain and serum readings of ß2m(r = 0.344, p = 0.005), TK (r = 0.263, p = 0.035), ICTP (r = 0.402, p = 0.001), PINP (r = 0.264, p = 0.039), sOPG (r =0.328, p = 0.028), sSyn-1 (r = 0.255, p = 0.046) and sFas (r = 0.418, p = 0.001). In case of K/L ratio there was found astatistically signifi cant association of levels of ß2m (r = 0.316, p = 0.01), TK (r = 0.274, p = 0.027), ICTP (r = 0.346, p =0.006), PINP (r = 0.261, p = 0.042), sSyn-1 (r = 0.283, p = 0.026) and sFas (r = 0.377, p = 0.002). In the lambda-groupthe analysis confi rmed mutual dependence of the dominant lambda chain levels on ß2m (r = 0.476, p = 0.003), ICTP(r = 0.375, p = 0.022), sVCAM-1 (r = 0.383, p = 0.019), sHGF (r = 0.441, p = 0.006) and sFas (r = 0.334, p = 0.040).In addition we ascertained a correlation of L/K ratio and levels of ß2m (r = –0.473, p = 0.003), TK (r = –0.412, p =0.011), ICTP (r = –0.331, p = 0.045), PINP (r = –0.409, p = 0.012), sHGF (r = –0.357, p = 0.028), sSyn-1 (r = –0.449,p = 0.005) a sFas (r = – 0.371, p = 0.022). Conclusions: The study confi rmed mutual correlation of FLC serum levels and the levels of several selected biologicalmarkers, in particular ß2m, TK, ICTP, PINP, sSyn-1 a sFas at time of the diagnosis. It referred to the mutualrelation of bone marrow microenvironment, biological qualities of clonal plasmocytes and the intensity of the freelight chains production by the tumour cell population.
- MeSH
- biologické markery krev MeSH
- diferenciální diagnóza MeSH
- financování organizované MeSH
- imunoanalýza metody využití MeSH
- imunoenzymatické techniky metody využití MeSH
- imunoglobuliny - kappa-řetězce izolace a purifikace MeSH
- imunoglobuliny - lambda-řetězce izolace a purifikace MeSH
- lehké řetězce imunoglobulinů izolace a purifikace MeSH
- lidé MeSH
- mnohočetný myelom diagnóza etiologie MeSH
- monoklonální gamapatie nejasného významu diagnóza MeSH
- progrese nemoci MeSH
- prospektivní studie MeSH
- radioimunoanalýza metody využití MeSH
- statistika jako téma MeSH
- Check Tag
- lidé MeSH