Oxidative stress markers are usually measured in plasma, a stable environment for biomarkers. Blood collection is invasive, but the use of alternative biofluids is limited, due to high variability. In this study, we aimed to establish reference values for oxidative stress markers in plasma, urine and saliva of adult, healthy mice and to identify some sources of variability. Samples were obtained from 41 female and 37 male adult, healthy mice of the CD-1 strain, aged 95-480 days, weighing 21-55 grams. Reference ranges of TBARS (thiobarbituric acid reactive substances), AOPP (advanced oxidation protein products), fructosamine, GSH/GSSG (reduced and oxidized glutathione) ratio, TAC (total antioxidant capacity), and FRAP (ferric reducing antioxidant power) were measured in plasma and urine, and TBARS, GSH/GSSG ratio, TAC and FRAP in saliva, using standard spectrophotometric and fluorometric methods. Salivary GSH/GSSG and urinary AOPP were higher in females. Urinary fructosamine, GSH/GSSG and FRAP were higher in males. Urinary TAC and FRAP negatively correlated with age, and urinary GSH/GSSG positively correlated with weight. We determined that urine and saliva can be obtained non-invasively from mice, in sufficient amounts for reliable oxidative status assessment. Further studies are needed to uncover whether these biofluids reflect systemic oxidative status in diseases.
- MeSH
- antioxidancia metabolismus MeSH
- biologické markery krev MeSH
- fruktosamin krev moč MeSH
- glutathion krev moč MeSH
- látky reagující s kyselinou thiobarbiturovou metabolismus MeSH
- myši MeSH
- oxidační stres fyziologie MeSH
- sliny metabolismus MeSH
- zdravotní stav * MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- elektrokardiografie využití MeSH
- endotelin-1 diagnostické užití terapeutické užití MeSH
- experimenty na zvířatech MeSH
- infarkt myokardu farmakoterapie MeSH
- koronární nemoc patofyziologie MeSH
- prospektivní studie MeSH
- psi MeSH
- srdeční arytmie patofyziologie MeSH
- zvířata MeSH
- Check Tag
- psi MeSH
- zvířata MeSH
- MeSH
- časové faktory MeSH
- dospělí MeSH
- estradiol krev metabolismus MeSH
- finanční podpora výzkumu jako téma MeSH
- Glycine max metabolismus MeSH
- kognice MeSH
- lidé MeSH
- potravní doplňky zásobování a distribuce MeSH
- psychologické testy MeSH
- testosteron krev metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- MeSH
- glukokortikoidy metabolismus MeSH
- neurodegenerativní nemoci farmakoterapie metabolismus MeSH
- NF-kappa B antagonisté a inhibitory fyziologie MeSH
- reaktivní formy kyslíku antagonisté a inhibitory MeSH
- transkripční faktory genetika MeSH
- tumor nekrotizující faktory fyziologie MeSH
- zánět farmakoterapie metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH