Long-term performance of implanted cardiovascular grafts can be ensured if living endothelium overgrows their surface. Surface modifications to implants are therefore being sought that can encourage endothelialization while preventing thrombus formation until the natural endothelium is formed. In the present study, heparin was covalently attached to a fibrin mesh grown from a polyvinyl chloride (PVC) substrate surface by the catalytic action of surface immobilized thrombin on a fibrinogen solution. The coating prevented platelet activation, thrombin generation and clot formation, and reduced inflammatory reactions when exposed to fresh human whole blood circulating in a Chandler loop model. In addition, in vitro seeded human umbilical vein and human saphenous vein endothelial cells showed considerably enhanced attachment and proliferation on the coating. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2995-3005, 2017.
- MeSH
- aktivace trombocytů účinky léků MeSH
- antikoagulancia chemie farmakologie MeSH
- biokompatibilní potahované materiály chemie farmakologie MeSH
- buněčná adheze účinky léků MeSH
- cévní protézy škodlivé účinky MeSH
- endoteliální buňky pupečníkové žíly (lidské) MeSH
- endoteliální buňky cytologie účinky léků MeSH
- fibrin chemie MeSH
- hematokrit MeSH
- hemokoagulace účinky léků MeSH
- heparin chemie farmakologie MeSH
- kultivované buňky MeSH
- lidé MeSH
- trombóza krev etiologie prevence a kontrola MeSH
- vena saphena cytologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
