- MeSH
- chronická nemoc MeSH
- hypoxie * patofyziologie MeSH
- maternofetální výměna látek fyziologie MeSH
- modely nemocí na zvířatech MeSH
- perfuze metody MeSH
- placenta krevní zásobení MeSH
- potkani Wistar * MeSH
- těhotenství MeSH
- zvířata MeSH
- Check Tag
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
The vessels on the fetal side of the placenta differ from most other vascular beds except the lungs in that they respond to acute hypoxia by vasoconstriction. An essential role of calcium influx in the mechanism of this hypoxic fetoplacental vasoconstriction (HFPV) has been shown previously. That finding does not, however, exclude the possible involvement of other mechanisms of vascular tone regulation. In this study we tested the hypothesis that Rho-kinase-mediated calcium sensitization is involved in HFPV. We used a model of isolated rat placenta dually perfused (from both the maternal and fetal side) with Krebs salt solution saturated with normoxic and hypoxic gas mixture respectively at constant flow rate. Rho-kinase pathway was inhibited by fasudil (10 μM). We found that fasudil reduced basal normoxic fetoplacental vascular resistance and completely prevented HFPV. This suggests that the activity of Rho-kinase signaling pathway is essential for HFPV.
- MeSH
- cévní rezistence fyziologie MeSH
- hypoxie metabolismus patofyziologie MeSH
- kinázy asociované s rho antagonisté a inhibitory metabolismus MeSH
- placenta * krevní zásobení MeSH
- placentární oběh * fyziologie MeSH
- těhotenství MeSH
- vápník MeSH
- vazokonstrikce fyziologie MeSH
- zvířata MeSH
- Check Tag
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- MeSH
- dextrany diagnostické užití MeSH
- lymfografie metody MeSH
- psi MeSH
- radioisotopová scintigrafie MeSH
- Check Tag
- psi MeSH
