The Central and Eastern European Proteomic Conference (CEEPC) successfully launched its second decade of proteomics in Košice, Slovakia with a program of systems biology, cellular, clinical, veterinary and sports proteomics. Whilst many conferences are struggling to attract participants, CEEPC with its outstanding track record and unique 'family - feel' packaged with excellent ambiance is thriving and bringing together proteomics experts from academia, industry, scientific specialties, clinics and precision medicine communities interested in resolving mysteries about protein functionalities in health and disease. CEEPC is also renowned for addressing humanitarian global healthcare issues, may it be ageing, chronic diseases or global epidemics. This year CEEPC intertwined with Košice Peace Marathon's pursuit of excellence in sports and initiatives including sports medicine and global peace.
This study presents the binding of ovine factor H (fH) by various serotypes of Borrelia and simultaneously correlates their complement resistance to sheep serum. Affinity ligand binding assay was employed to study the binding of borrelial proteins to ovine recombinant fH and its truncated forms (short consensus repeat, SCR 7 and SCRs 19-20). From a repertoire of 17 borrelial strains, only two strains showed affinity to sheep fH. A ~28-kDa protein of Borrelia burgdorferi sensu stricto (B. burgdorferi s.s., strain SKT-2) bound full-length fH as well as SCRs 19-20. This fH-binding protein was further identified as complement regulator-acquiring surface protein of B. burgdorferi (BbCRASP-1) by MALDI-TOF analysis. Surprisingly, a ~26-kDa protein of Borrelia bissettii (DN127) showed affinity to full-length fH but not to SCR 7 and SCRs19-20. In complement sensitivity assay, both strains-SKT-2 and DN127-were resistant to normal sheep serum. Significant complement resistance of two Borrelia garinii strains (G117 and T25) was also observed; however, none of those strains was able to bind sheep fH. Our study underscores the need of further exploration of fH-mediated evasion of complement system by Borrelia in domestic animals.
- MeSH
- Bacterial Proteins chemistry genetics immunology MeSH
- Borrelia classification genetics immunology isolation & purification MeSH
- Kinetics MeSH
- Complement Factor H chemistry immunology MeSH
- Lyme Disease immunology microbiology veterinary MeSH
- Molecular Sequence Data MeSH
- Sheep Diseases immunology microbiology MeSH
- Sheep MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- MeSH
- Complement Pathway, Alternative immunology MeSH
- Blood Bactericidal Activity drug effects MeSH
- Borrelia immunology MeSH
- Financing, Organized MeSH
- Borrelia Infections immunology blood veterinary MeSH
- Humans MeSH
- Disease Reservoirs classification veterinary MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- MeSH
- Borrelia immunology MeSH
- Research Support as Topic MeSH
- Lyme Disease diagnosis veterinary MeSH
- Sheep Diseases history MeSH
- Dog Diseases microbiology MeSH
- Nerve Tissue Proteins diagnostic use MeSH
- Staphylococcal Protein A diagnostic use MeSH
- Deer microbiology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Geographicals
- Slovakia MeSH
