In eukaryotes, the decapping machinery is highly conserved and plays an essential role in controlling mRNA stability, a key step in the regulation of gene expression. Yet, the role of mRNA decapping in shaping gene expression profiles in response to environmental cues and the operating molecular mechanisms are poorly understood. Here, we provide genetic and molecular evidence that a component of the decapping machinery, the LSM1-7 complex, plays a critical role in plant tolerance to abiotic stresses. Our results demonstrate that, depending on the stress, the complex from Arabidopsis thaliana interacts with different selected stress-inducible transcripts targeting them for decapping and subsequent degradation. This interaction ensures the correct turnover of the target transcripts and, consequently, the appropriate patterns of downstream stress-responsive gene expression that are required for plant adaptation. Remarkably, among the selected target transcripts of the LSM1-7 complex are those encoding NCED3 and NCED5, two key enzymes in abscisic acid (ABA) biosynthesis. We demonstrate that the complex modulates ABA levels in Arabidopsis exposed to cold and high salt by differentially controlling NCED3 and NCED5 mRNA turnover, which represents a new layer of regulation in ABA biosynthesis in response to abiotic stress. Our findings uncover an unanticipated functional plasticity of the mRNA decapping machinery to modulate the relationship between plants and their environment.

• MeSH
• Arabidopsis genetika   fyziologie   MeSH
• biologické modely * MeSH
• chlorid sodný metabolismus   MeSH
• endoribonukleasy genetika   metabolismus   MeSH
• fyziologická adaptace * MeSH
• fyziologický stres MeSH
• geneticky modifikované rostliny MeSH
• kyselina abscisová metabolismus   MeSH
• messenger RNA genetika   metabolismus   MeSH
• nízká teplota MeSH
• proteiny huseníčku genetika   metabolismus   MeSH
• regulace genové exprese u rostlin * MeSH
• regulátory růstu rostlin metabolismus   MeSH
• reportérové geny MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH

The strategies used by living organisms to survive under low and freezing temperatures reveal the extraordinary adaptability of life on Earth. Understanding the molecular mechanisms underlying cold adaptation and freezing survival will provide new insights into the existing relationships between living organisms and their environment, and the possibility of developing multiple biotechnological applications. In the case of plants, the use of classical genetic and new "omics" approaches is allowing to the identification of new elements involved in regulating the cold acclimation response. The challenge ahead is to determine temperature-perception molecules and mechanisms, to uncover new internodes of multiple responses, and to integrate the regulation not only at the transcriptome but also at proteome and metabolome levels. Attaining these goals will significantly contribute global understanding the adaptive strategies plants have evolved to cope with hostile environmental conditions, and to the development biotechnological strategies to improve crop tolerance to freezing and other important abiotic stresses.

• MeSH
• adaptorové proteiny signální transdukční genetika   imunologie   MeSH
• aklimatizace fyziologie   genetika   imunologie   MeSH
• Arabidopsis cytologie   genetika   imunologie   MeSH
• biologická adaptace fyziologie   genetika   imunologie   MeSH
• biotechnologie metody   trendy   MeSH
• cytosol chemie   metabolismus   MeSH
• financování organizované MeSH
• fyziologická adaptace fyziologie   genetika   imunologie   MeSH
• metabolomika metody   MeSH
• molekulární biologie metody   MeSH
• nízká teplota škodlivé účinky   MeSH
• permeabilita buněčné membrány fyziologie   genetika   imunologie   MeSH
• proteomika metody   MeSH
• regulace genové exprese genetika   imunologie   MeSH
• rostliny MeSH
• Publikační typ
• přehledy MeSH