The aim of the study was to assess serum hyaluronic acid (HA) and transforming growth factor beta 1 (TGF-β1) concentrations: 1) to differentiate hepatic fibrosis from other forms of liver disease, and 2) for the non-invasive staging of canine liver fibrosis. We also evaluated the association between serum HA concentration and the size of the shunt vessel as an indirect marker of decreased liver clearance in patients with single congenital vascular anomaly. Forty-one healthy client-owned dogs and forty dogs diagnosed with hepatobiliary disease were enrolled in the prospective study. Patients were divided into 4 subgroups: 1) congenital portosystemic shunts (CPSS); 2) parenchymal diseases (a. mild and moderate fibrosis, b. advanced fibrosis and cirrhosis); 3) hepatic neoplasia; 4) biliary tract disorders based on thorough clinical, ultrasound and histopathological examination. Serum HA and TGF-β1 concentrations were measured using ELISA. The HA concentration was significantly increased in patients with advanced liver fibrosis/cirrhosis (P < 0.001) and CPSS (P < 0.001) compared to healthy dogs. Using a cut-off HA concentration of 135.94 ng/ml, the sensitivity and specificity for diagnosis for advanced liver fibrosis/cirrhosis was 100% (95% CI, 50.6–100) and 90.8% (95% CI, 81.6–95.7), respectively. The TGF-β1 levels did not significantly differ among groups (P = 0.180). Negligible correlation was found between serum HA concentration and the size of portosystemic shunt vessel (rs = 0.07; P = 0.831). These findings suggest that serum HA concentration is a potential non-invasive biomarker for advanced liver fibrosis and/or cirrhosis in dogs. The utility of measuring serum concentration of TGF-β1 for diagnosing canine liver fibrosis was not supported.

• MeSH
• biologické markery krev   MeSH
• biopsie metody   MeSH
• chronická hepatitida krev   veterinární   MeSH
• diferenciální diagnóza MeSH
• ELISA MeSH
• jaterní cirhóza diagnóza   krev   veterinární   MeSH
• kyselina hyaluronová * krev   MeSH
• laparoskopie metody   MeSH
• nemoci jater diagnóza   klasifikace   krev   veterinární   MeSH
• nemoci trávicího systému diagnóza   klasifikace   krev   veterinární   MeSH
• prospektivní studie MeSH
• psi MeSH
• ROC křivka MeSH
• senzitivita a specificita MeSH
• statistika jako téma MeSH
• transformující růstový faktor beta1 * krev   MeSH
• Check Tag
• psi MeSH
• Publikační typ
• práce podpořená grantem MeSH

• MeSH
• kardiovaskulární nemoci veterinární   MeSH
• kastrace škodlivé účinky   veterinární   MeSH
• kočky MeSH
• komorbidita * MeSH
• krmivo pro zvířata MeSH
• muskuloskeletální nemoci veterinární   MeSH
• nadváha veterinární   MeSH
• obezita * epidemiologie   komplikace   veterinární   MeSH
• pouto mezi člověkem a zvířetem MeSH
• psi MeSH
• rizikové faktory * MeSH
• zvířata MeSH
• Check Tag
• kočky MeSH
• psi MeSH
• zvířata MeSH
• Publikační typ
• přehledy MeSH

Trichomonads are a group of anaerobic flagellates. Two species of intestinal trichomonads have been described in cats - pathogenic Tritrichomonas foetus and probably harmless Pentatrichomonas hominis. InPouch™ TF-Feline medium (Bio-Med Diagnostics, White City, Oregon, USA) is considered the gold standard for diagnosis of T. foetus infection in cats. It is commercially available, relatively cheap and easy-to-use. The medium is intended to be highly specific to T. foetus as morphologically similar Pentatrichomonas hominis and Giardia sp. do not survive here longer than 24h. In this study we successfully cultivated P. hominis in InPouch™ TF-Feline medium for 3 days after inoculation with cat faeces. The identity of the organism was assessed by sequencing of SSU rDNA and ITS region. Possible coinfection with T. foetus was ruled out using Tritrichomonas-specific PCR. Our results suggest possible misdiagnosis of tritrichomonosis in cats using InPouch™ TF-Feline medium. PCR-based verification of culture-positive samples prior the potentially neurotoxic ronidazole treatment is recommended.

• MeSH
• feces parazitologie   MeSH
• kočky MeSH
• kultivační média MeSH
• nemoci koček diagnóza   parazitologie   MeSH
• protozoální infekce zvířat diagnóza   parazitologie   MeSH
• Tritrichomonas foetus izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• kočky MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH