The aim of the study was to assess serum hyaluronic acid (HA) and transforming growth factor beta 1 (TGF-β1) concentrations: 1) to differentiate hepatic fibrosis from other forms of liver disease, and 2) for the non-invasive staging of canine liver fibrosis. We also evaluated the association between serum HA concentration and the size of the shunt vessel as an indirect marker of decreased liver clearance in patients with single congenital vascular anomaly. Forty-one healthy client-owned dogs and forty dogs diagnosed with hepatobiliary disease were enrolled in the prospective study. Patients were divided into 4 subgroups: 1) congenital portosystemic shunts (CPSS); 2) parenchymal diseases (a. mild and moderate fibrosis, b. advanced fibrosis and cirrhosis); 3) hepatic neoplasia; 4) biliary tract disorders based on thorough clinical, ultrasound and histopathological examination. Serum HA and TGF-β1 concentrations were measured using ELISA. The HA concentration was significantly increased in patients with advanced liver fibrosis/cirrhosis (P < 0.001) and CPSS (P < 0.001) compared to healthy dogs. Using a cut-off HA concentration of 135.94 ng/ml, the sensitivity and specificity for diagnosis for advanced liver fibrosis/cirrhosis was 100% (95% CI, 50.6–100) and 90.8% (95% CI, 81.6–95.7), respectively. The TGF-β1 levels did not significantly differ among groups (P = 0.180). Negligible correlation was found between serum HA concentration and the size of portosystemic shunt vessel (rs = 0.07; P = 0.831). These findings suggest that serum HA concentration is a potential non-invasive biomarker for advanced liver fibrosis and/or cirrhosis in dogs. The utility of measuring serum concentration of TGF-β1 for diagnosing canine liver fibrosis was not supported.
- MeSH
- biologické markery krev MeSH
- biopsie metody MeSH
- chronická hepatitida krev veterinární MeSH
- diferenciální diagnóza MeSH
- ELISA MeSH
- jaterní cirhóza diagnóza krev veterinární MeSH
- kyselina hyaluronová * krev MeSH
- laparoskopie metody MeSH
- nemoci jater diagnóza klasifikace krev veterinární MeSH
- nemoci trávicího systému diagnóza klasifikace krev veterinární MeSH
- prospektivní studie MeSH
- psi MeSH
- ROC křivka MeSH
- senzitivita a specificita MeSH
- statistika jako téma MeSH
- transformující růstový faktor beta1 * krev MeSH
- Check Tag
- psi MeSH
- Publikační typ
- práce podpořená grantem MeSH
Cholinesterasy jsou enzymy nezastupitelné v některých nervových přenosech, kde hraje zásadní roli acetylcholinesterasa. Druhá cholinesterasa, butyrylcholinesterasa, pak není nezbytná pro neurotransmisi, ale může se podílet na některých detoxifikačních reakcích. V tomto přehledovém článku je provedeno shrnutí literatury, diskuze diagnostického významu a metod určení aktivity. Je tak nastíněno například rozpoznání jaterních selhání, expozice neurotoxickým látkám, geneticky podmíněné dispozice. V oblasti testování je diskutováno provádění spektrofotometrických, kolorimetrických a elektrochemických měření.
Cholinesterases are enzymes important for some nerve transmissions where the enzyme acetylcholinesterase plays a crucial role. The second enzyme, butyrylcholinesterase, is not necessary for the neurotransmission but it is involved in some detoxification reactions. A survey of literature, a discussion of diagnostic importance and the methods for an activity assay are presented in this review article. Liver failures, exposure to neurotoxic compounds, genetic dispositions are outlined here. In the field of assays, spectrophotometric, colorimetric and electrochemical tests are discussed.
