Ischemická choroba srdeční je nejčastější kardiovaskulární příčinou úmrtí v České republice. Její těžké formy mohou vést až k srdečnímu selhání, které má až 50% čtyřletou mortalitu. V dnešní době můžeme prognózu výrazně zlepšit moderní farmakologickou léčbou. Jedněmi z pilířů této léčby jsou inhibitory ACE a β-blokátory, které výrazně snižují morbiditu i mortalitu pacientů. Klíčem k úspěchu je adherence pacientů k léčbě, po dvou letech však předepsanou léčbu nedodržují až dvě třetiny pacientů. Adherenci lze zlepšit podáváním kombinovaných léčivých přípravků. V České republice je jediným dostupným kombinovaným léčivem s obsahem inhibitoru ACE i β-blokátoru bisoprolol/perindopril. Formou kazuistiky prezentujeme výhodu užívání bisoprolol/perindoprilu u non-adherentní pacientky s ICHS a srdečním selháním.
Coronary artery disease is the most common cardiovascular cause of death in Czech Republic. The most severe forms of CAD can lead to heart failure, a serious condition with poor prognosis and a 50% four-year mortality. Nowadays, prognosis can be significantly improved by modern medication. The core of pharmacologic therapy are ACE-inhibitors and betablockers, which vastly reduce both morbidity and mortality. The key to success is adherence, up to two thirds of patients are not following proper treatment protocol after two years. Adherence can be improved by using polypills. The only available polypill combining ACE inhibitor and betablocker in the Czech Republic is bisoprolol/perindopril. In this case report, we show the benefit of prescribing of bisoprolol/perindopril to a non-adherent patient with CAD and heart failure
- MeSH
- adherence k farmakoterapii statistika a číselné údaje MeSH
- beta blokátory aplikace a dávkování farmakologie MeSH
- bisoprolol aplikace a dávkování farmakologie MeSH
- diferenciální diagnóza MeSH
- echokardiografie MeSH
- fixní kombinace léků * MeSH
- inhibitory ACE aplikace a dávkování farmakologie MeSH
- komorbidita MeSH
- koronární angiografie MeSH
- lidé MeSH
- nemoci srdce * diagnostické zobrazování farmakoterapie terapie MeSH
- neúspěšná terapie MeSH
- perindopril aplikace a dávkování farmakologie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
AIM: Cloning of artificial intronic sequence within the open reading frame (ORF) of DsRed2 gene. METHOD: Splice prediction software was used to analyze DsRed2 sequence to find an ideal site for cloning artificial intronic sequence. Intron was cloned within DsRed2 using cyclic ligation assembly. Flow cytometry was used to quantify the number of cells expressing red fluorescence. RESULT: Sequencing data confirmed precise cloning of intron at the desired position using cyclic ligation assembly. Successful expression of red fluorescence after cloning of intron confirmed successful intron recognition and splicing by host cell line. Cloning of intron increased the number of cells expressing red fluorescent protein. CONCLUSION: Cloning of intronic sequence within DsRed2 has helped to increase the number of cells expressing red fluorescence by approximately four percent.
- MeSH
- HeLa buňky MeSH
- introny MeSH
- klonování DNA metody MeSH
- lidé MeSH
- luminescentní proteiny genetika metabolismus MeSH
- otevřené čtecí rámce MeSH
- transfekce MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Ensuring mycoplasma-free cell culture is of prime importance as they severely affect cellular characteristics leading to experimental artefacts and spurious results. Various methods persist for mycoplasma detection; out of the whole array of methods polymerase chain reaction (PCR) is the most favoured one because it is highly sensitive, specific and quick. The PCR-based detection procedure involves three steps: cell culture supernatant collection, DNA isolation, and PCR. We have modified this procedure so that cell culture supernatant can directly be used for PCR without the need for DNA extraction. This modification makes the procedure quicker and more sensitive because loss of mycoplasma DNA is prevented and this loss becomes more significant when the level of mycoplasma contamination is very low.
- MeSH
- buněčné linie MeSH
- kontaminace DNA * MeSH
- lidé MeSH
- Mycoplasma genetika izolace a purifikace MeSH
- myši MeSH
- polymerázová řetězová reakce metody MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The cell culture became an invaluable tool for studying cell behaviour, development, function, gene expression, toxicity of compounds and efficacy of novel drugs. Although most results were obtained from cell cultivation in two-dimensional (2D) systems, in which cells are grown in a monolayer, three-dimensional (3D) cultures are more promising as they correspond closely to the native arrangement of cells in living tissues. In our study, we focused on three types of 3D in vitro systems used for cultivation of one cell type. Cell morphology, their spatial distribution inside of resulting multicellular structures and changes in time were analysed with histological examination of samples harvested at different time periods. In multilayered cultures of WRL 68 hepatocytes grown on semipermeable membranes and non-passaged neurospheres generated by proliferation of neural progenitor cells, the cells were tightly apposed, showed features of cell differentiation but also cell death that was observable in short-term cultures. Biogenic scaffolds composed of extracellular matrix of the murine tibial anterior muscle were colonized with C2C12 myoblasts in vitro. The recellularized scaffolds did not reach high cell densities comparable with the former systems but supported well cell anchorage and migration without any signs of cell regression.
- MeSH
- buněčné kultury MeSH
- buněčné linie MeSH
- buněčné sféroidy fyziologie MeSH
- hepatocyty fyziologie MeSH
- lidé MeSH
- myoblasty MeSH
- myši MeSH
- proliferace buněk fyziologie MeSH
- tkáňové inženýrství metody MeSH
- tkáňové podpůrné struktury * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
