- MeSH
- potravinářská technologie MeSH
- potravinářský průmysl MeSH
- syrovátka * MeSH
- Publikační typ
- práce podpořená grantem MeSH
- MeSH
- antibakteriální látky * farmakologie MeSH
- Candida tropicalis účinky léků MeSH
- Escherichia coli účinky léků MeSH
- mikrobiální testy citlivosti metody MeSH
- Penicillium chrysogenum účinky léků MeSH
- potravinářská mikrobiologie MeSH
- rostlinné extrakty * MeSH
- techniky in vitro MeSH
- těkavé organické sloučeniny * farmakologie MeSH
- Thymus (rostlina) chemie MeSH
- Publikační typ
- práce podpořená grantem MeSH
The aim of this in vitro study was to examine the secretion activity (progesterone, 17beta-estradiol and insulin-like growth factor-I) of rat ovarian fragments after molybdenum (Mo) addition. Rat ovarian fragments were incubated with ammonium molybdate (NH(4))(6)Mo(7)O(24).4H(2)O at the doses 90, 170, 330 and 500 microg.ml(-1) for 24 h and compared with control group without Mo addition. Release of progesterone (P(4)), estradiol (17beta-estradiol) and insulin-like growth factor I (IGF-I) by ovarian fragments was assessed by radioimmunoassay (RIA). Data show that P(4) release by ovarian fragments was not affected by (NH(4))(6).Mo(7)O(24).4H(2)O addition at all the doses used (90-500 microg.ml(-1)). However, addition of ammonium molybdate was found to cause a significant (P<0.05) dose-dependent decrease (at the doses 90, 170 and 500 microg.ml(-1)) in release of 17beta-estradiol by ovarian fragments in comparison to control. Also, addition of ammonium molybdate significantly (P<0.05) inhibited IGF-I release at all the doses (90-500 microg.ml(-1)) used in the study. Results suggest ammonium molybdate induced inhibition in the release of growth factor IGF-I and its dose-dependent effect on secretion of steroid hormone 17beta-estradiol but not progesterone. These data contribute to new insights regarding the mechanism of action of Mo on rat ovarian functions.
- MeSH
- estradiol sekrece MeSH
- insulinu podobný růstový faktor I sekrece MeSH
- krysa rodu rattus MeSH
- molybden farmakologie MeSH
- ovarium účinky léků sekrece MeSH
- progesteron sekrece MeSH
- techniky in vitro MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
