Head and neck cancer is the sixth most common malignancy worldwide, predominantly developing from squamous cell epithelia (HNSCC). The main HNSCC risk factors are tobacco, excessive alcohol use, and the presence of human papillomavirus (HPV). HPV positive (+) cancers are etiologically different from other HNSCC and often show better prognosis. The current knowledge regarding HNSCC miRNA profiles is still incomplete especially in the context of HPV+ cancer. Thus, we analyzed 61 freshly collected primary oral (OSCC) and oropharyngeal (OPSCC) SCC samples. HPV DNA and RNA was found in 21% cases. The Illumina whole-genome small-RNA profiling by next-generation sequencing was done on 22 samples and revealed 7 specific miRNAs to HPV+ OSCC, 77 to HPV+ OPSCC, and additional 3 shared with both; 51 miRNAs were specific to HPV- OPSCC, 62 to HPV- OSCC, and 31 shared with both. The results for 9 miRNAs (miR-9, -21, -29a, -100, -106b, -143 and -145) were assessed by reverse transcription-quantitative polymerase chain reaction on the whole study population. The data was additionally confirmed by reanalyzing publicly available miRNA sequencing Cancer Genome Atlas consortium (TCGA) HNSCC data. Cell signaling pathway analysis revealed differences between HPV+ and HPV- HNSCC. Our findings compared with literature data revealed extensive heterogeneity of miRNA deregulation with only several miRNAs consistently affected, and miR-9 being the most likely HPV related miRNA.

• MeSH
• dospělí MeSH
• infekce papilomavirem genetika   virologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mikro RNA genetika   metabolismus   MeSH
• nádorové biomarkery genetika   MeSH
• nádory hlavy a krku genetika   virologie   MeSH
• nádory orofaryngu genetika   virologie   MeSH
• Papillomaviridae genetika   patogenita   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• techniky in vitro MeSH
• vysoce účinné nukleotidové sekvenování MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

BACKGROUND: Human papillomaviruses (HPV) are a heterogeneous group of viruses classified into five genera. The beta-HPV type (beta-PV) infection is very common but mostly asymptomatic in immunocompetent individuals. However, beta-PVs play a role in Epidermodysplasia verruciformis and possibly in non-melanoma skin cancer. Head and neck cancer (HNC) is a common cancer type worldwide and high-risk alpha-PV involvement in HNC has been extensively studied but beta-PV types have rarely been the focus of such studies. OBJECTIVES: To evaluate the prevalence of beta-PV types in HNC, subjects with non-malignant or potentially pre-malignant oral lesions, and healthy controls. STUDY DESIGN: The frequency of different beta-PVs in samples from oral (n=35) and oropharyngeal (n=35) cancer patients, gender- and age-matched healthy controls (n=70), and subjects with various non-malignant or potentially pre-malignant oral lesions (n=102) was assessed by a highly sensitive, bead-based, multiplex genotyping assay. RESULTS: Overall, 54.8% of all tested samples contained at least one beta-PV type. Even though the correlation between types found in lavage and tissue specimens from cancer patients was low, there was a large statistically significant difference between oropharyngeal cancer patients and matched controls for HPV5 (P=0.003; OR=15.58) and between both oral (P=0.026; OR=5.7) and oropharyngeal cancer patients (P=0.002; OR=25.5) and controls for HPV122. In addition, there was no correlation between the prevalence of alpha and beta-PVs in the study patients. CONCLUSION: The study provides new data on the prevalence of beta-PVs in HNC. HPV5 was found significantly associated with HNC as already observed by other studies. Additionally, the significant association of HPV122 with HNC might warrant further study as this type has not been extensively studied so far.

• MeSH
• Betapapillomavirus klasifikace   genetika   izolace a purifikace   MeSH
• dospělí MeSH
• genotyp * MeSH
• genotypizační techniky MeSH
• infekce papilomavirem epidemiologie   virologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• nemoci úst epidemiologie   virologie   MeSH
• orofarynx virologie   MeSH
• prevalence MeSH
• senioři MeSH
• ústa virologie   MeSH
• zdraví dobrovolníci pro lékařské studie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• Publikační typ
• abstrakt z konference MeSH

The situation of cervical cancer prevention in Croatia is hardly well established, in spite of the fact that opportunistic screening is on going for some time and that cervical cancer is on the 8th place of female cancer. Each year, approximately 350 women develop cervical cancer and about 100 die from the disease. A matter of concern is the steady state of the mortality rate. Thus, appropriate cervical cancer prevention programmes should be set up without delay in Croatia.

• MeSH
• cytologické techniky dějiny   metody   využití   MeSH
• infekce papilomavirem diagnóza   epidemiologie   prevence a kontrola   MeSH
• kolposkopie metody   využití   MeSH
• lidé MeSH
• nádory děložního čípku diagnóza   prevence a kontrola   MeSH
• plošný screening MeSH
• směrnice pro lékařskou praxi jako téma MeSH
• statistika jako téma MeSH
• vaginální stěr metody   využití   MeSH
• vakcíny proti papilomavirům terapeutické užití   MeSH
• vládní programy organizace a řízení   MeSH
• Check Tag
• lidé MeSH
• Geografické názvy
• Chorvatsko MeSH

Human papillomaviruses (HPVs) have been recognized as etiologic factors in a variety of diseases. Due to the large number of HPV types, methods for HPV genotyping are difficult to standardize. Despite this fact, several methods exist, and some of them are available commercially. In this study, we evaluated the Roche Diagnostics linear array (LA) HPV genotyping assay, the Innogenetics INNO-LiPA (line probe assay [LiPA]), and two noncommercial reverse line blot (RLB) assays based on either primers GP5+ and GP6+ (GP) or newly designed broad-spectrum primers BSGP5+ and BSGP6+ (BS). The reliabilities of these assays were tested with a wide spectrum of HPV types less prevalent in cervical samples. This is the first study to compare the performance of the most widely used HPV genotyping methods with selected samples positive for low-prevalence HPV types. We focused on interassay agreement, both overall and type specific, in cases with single and/or multiple HPV infections. Interassay agreement was moderate in cases of single HPV infections and poor in cases of multiple HPV infections. The LA and the BS-based RLB assays found a higher rate of cases positive for multiple HPV types than LiPA and the GP-based RLB assay. The weakest capability in detecting multiple HPV infections was observed for LiPA. The use of only one assay in epidemiological and clinical studies might lead to biased conclusions. Therefore, a universally evaluated and agreed upon HPV typing assay or a combination of current assays is needed for possible clinical applications, and knowledge of their limitations is advised.

• MeSH
• diagnostické techniky molekulární * metody   MeSH
• infekce papilomavirem * diagnóza   virologie   MeSH
• lidé MeSH
• Papillomaviridae genetika   izolace a purifikace   klasifikace   MeSH
• senzitivita a specificita MeSH
• virologie * metody   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

National and international experts in cervical cancer prevention met at the International Workshop on Human Papillomaviruses and Consensus Recommendations for Cervical Cancer Prevention to review the current evidence and assess the potential for improvement in cervical cancer prevention and to develop plans for implementation of cervical cancer prevention programmes in Croatia. Key recommendations were developed and adopted during the course of the meeting. The process of bringing national experts together with internationally recognized experts in an open forum for the development of consensus recommendations could serve as a model for other countries seeking to implement or improve cervical cancer prevention programmes.

• MeSH
• dysplazie děložního hrdla diagnóza   prevence a kontrola   diagnóza   prevence a kontrola   MeSH
• Evropská unie MeSH
• nádory děložního čípku diagnóza   prevence a kontrola   MeSH
• plošný screening metody   organizace a řízení   využití   MeSH
• směrnice pro lékařskou praxi jako téma MeSH
• vakcíny proti papilomavirům aplikace a dávkování   terapeutické užití   MeSH
• Geografické názvy
• Chorvatsko MeSH