Ateroskleróza je hlavnou prí činou kardiovaskulá rnych ochorení a významne prispieva k celosvetovej úmrtnosti. Stá le rastúci počet dôkazov naznačuje, že črevný mikrobióm môže zohrá vať kľúčovú úlohu v patogenéze tohto ochorenia. Črevný mikrobióm pozostá va z biliónov mikroorganizmov, ktoré nielen podporujú trá venie a metabolizmus, ale tiež ovplyvňujú imunitnú odpoveď hostiteľa. Zmeny v zložení črevného mikrobiómu boli spojené s rôznymi chronickými ochoreniami, vrá tane obezity, cukrovky a aterosklerózy. Tento člá nok poskytuje stručný prehľad zloženia črevného mikrobiómu u pacientov s koroná rnou chorobu srdca a sumarizuje možné zá kladné mechanizmy vplyvu na rozvoj aterosklerózy. Zloženie črevného mikrobiómu u pacientov s aterosklerózou sa líš i od zdravých jedincov. Identifiká cia a pochopenie týchto rozdielov poskytuje nové možnosti pre diagnostiku a liečbu aterosklerózy.
Atherosclerosis is a major cause of cardiovascular diseases and significantly contributes to global mortality. An increasing body of evidence suggests that the gut microbiome may play a key role in the pathogenesis of this disease. The gut microbiome consists of trillions of microorganisms that not only support digestion and metabolism but also influence the host's immune response. Alterations in the composition of the gut microbiome have been associated with various chronic diseases, including obesity, diabetes, and atherosclerosis. This article provides a brief overview of the gut microbiome composition in patients with coronary artery disease and summarizes the potential underlying mechanisms influencing the development of atherosclerosis. The composition of the gut microbiome in patients with atherosclerosis differs from that in healthy individuals. Identifying and understanding these differences offers new opportunities for the diagnosis and treatment of atherosclerosis.
- MeSH
- arterioskleróza * etiologie prevence a kontrola MeSH
- lidé MeSH
- střevní mikroflóra * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
A novel series of trisubstituted acridines were synthesized with the aim of mimicking the effects of BRACO19. These compounds were synthesized by modifying the molecular structure of BRACO19 at positions 3 and 6 with heteroacyclic moieties. All of the derivatives presented in the study exhibited stabilizing effects on the human telomeric DNA quadruplex. UV-vis spectroscopy, circular dichroism, linear dichroism and viscosimetry were used in order to study the nature of the DNA binding in more detail. The results show that all of the novel derivatives were able to fold the single-stranded DNA sequences into antiparallel G-quadruplex structures, with derivative 15 exhibiting the highest stabilizing capability. Cell cycle analysis revealed that a primary trend of the "braco"-like derivatives was to arrest the cells in the S- and G2M-phases of the cell cycle within the first 72h, with derivative 13 and BRACO19 proving particularly effective in suppressing cell proliferation. All studies derivatives were less toxic to human fibroblast cell line in comparison with HT 29 cancer cell line.
- MeSH
- akridiny chemie farmakologie MeSH
- buněčné linie MeSH
- buněčný cyklus účinky léků MeSH
- DNA chemie metabolismus MeSH
- G-kvadruplexy účinky léků MeSH
- lidé MeSH
- ligandy MeSH
- nádorové buněčné linie MeSH
- nádory farmakoterapie metabolismus MeSH
- proliferace buněk účinky léků MeSH
- protinádorové látky chemie farmakologie MeSH
- simulace molekulového dockingu MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Synovial membrane and synovial fluid represent a good source of mesenchymal stem cells. They have been regarded as a promising therapeutic tool for musculoskeletal regeneration. Synovium-derived mesenchymal stem cells have higher expression of CD44 and better chondrogenic potential in vitro than mesenchymal stem cells from other tissues. In this study we compared mesenchymal stem cells from synovium and synovial fluid on the base of morphological, immunophenotype and differentiation features. A heterogeneous population of cells with different morphology was obtained after isolation and 4-day cultivation. The mesenchymal stem cell immunophenotype was confirmed by positive expression of CD105, CD90, and CD44 by flow cytometry and cells were negative for CD45. CD105+ cells were selected by immunomagnetic separation after 2–4 weeks of cultivation. The percentage of CD105+ cells in the mesenchymal stem cell population from synovia was between 40–50 % before immunomagnetic separation and increased to 95 % following the immunomagnetic separation. Von Kossa, Alcian blue and Oil Red O staining was used to assess the differentiation potential of synovial mesenchymal stem cells. Long-term cultivation did not affect the morphology and immunophenotype of synovial mesenchymal stem cells. Our results confirmed that immunomagnetic separation based on CD 105 antigen is a suitable method to enrich the subpopulation of CD105+ synovial mesenchymal stem cells.
- MeSH
- buněčná diferenciace MeSH
- buněčný rodokmen MeSH
- CD antigeny metabolismus MeSH
- chondrocyty cytologie MeSH
- chondrogeneze MeSH
- imunofenotypizace MeSH
- imunomagnetická separace metody MeSH
- lidé MeSH
- mezenchymální kmenové buňky cytologie metabolismus MeSH
- průtoková cytometrie MeSH
- receptory buněčného povrchu metabolismus MeSH
- separace buněk metody MeSH
- synoviální membrána cytologie MeSH
- synoviální tekutina cytologie MeSH
- Check Tag
- lidé MeSH
