Research on ancient and forensic DNA is related in many ways, and the two fields must deal with similar obstacles. Therefore, communication between these two communities has the potential to improve results in both research fields. Here, we present the insights gained in the ancient DNA community with regard to analyzing DNA from aged skeletal material and the potential use of the developed protocols in forensic work. We discuss the various steps, from choosing samples for DNA extraction to deciding between classical PCR amplification and massively parallel sequencing approaches. Based on the progress made in ancient DNA analyses combined with the requirements of forensic work, we suggest that there is substantial potential for incorporating ancient DNA approaches into forensic protocols, a process that has already begun to a considerable extent. However, taking full advantage of the experiences gained from ancient DNA work will require comparative studies by the forensic DNA community to tailor the methods developed for ancient samples to the specific needs of forensic studies and case work. If successful, in our view, the benefits for both communities would be considerable.
- MeSH
- DNA fingerprinting MeSH
- DNA * genetika MeSH
- lidé MeSH
- nekrotická degradace DNA MeSH
- senioři MeSH
- soudní genetika MeSH
- starobylá DNA * MeSH
- Check Tag
- lidé MeSH
- senioři MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
The Asiatic wild dog (Cuon alpinus), restricted today largely to South and Southeast Asia, was widespread throughout Eurasia and even reached North America during the Pleistocene. Like many other species, it suffered from a huge range loss towards the end of the Pleistocene and went extinct in most of its former distribution. The fossil record of the dhole is scattered and the identification of fossils can be complicated by an overlap in size and a high morphological similarity between dholes and other canid species. We generated almost complete mitochondrial genomes for six putative dhole fossils from Europe. By using three lines of evidence, i.e., the number of reads mapping to various canid mitochondrial genomes, the evaluation and quantification of the mapping evenness along the reference genomes and phylogenetic analysis, we were able to identify two out of six samples as dhole, whereas four samples represent wolf fossils. This highlights the contribution genetic data can make when trying to identify the species affiliation of fossil specimens. The ancient dhole sequences are highly divergent when compared to modern dhole sequences, but the scarcity of dhole data for comparison impedes a more extensive analysis.
- MeSH
- Canidae anatomie a histologie klasifikace genetika MeSH
- fylogeneze * MeSH
- genom mitochondriální MeSH
- hybridizace genetická MeSH
- migrace zvířat MeSH
- mitochondriální DNA MeSH
- starobylá DNA * MeSH
- zkameněliny MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Evropa MeSH
- MeSH
- bioinženýrství * MeSH
- DNA * analýza MeSH
- hemoglobiny analýza MeSH
- lidé MeSH
- mitochondriální DNA genetika MeSH
- polymerázová řetězová reakce MeSH
- receptor melanokortinový typ 1 genetika MeSH
- sekvenční analýza DNA MeSH
- sloni genetika MeSH
- zkameněliny * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH