- Publication type
- Meeting Abstract MeSH
Enterocytozoon bieneusi known as a causative agent of opportunistic infections instigating diarrhoea in AIDS patients was identified also in a number of immunocompetent patients and in a wide range of animals, including cattle. In the present study we tested if the Bovine Viral Diarrhea Virus (BVDV), the most common pathogen underlying immunosuppressive Bovine Viral Diarrhoea (BVD), can enhance the occurrence of opportunistic infections with E. bieneusi in cattle. Six dairy farms were investigated using ELISA to detect antibodies against or antigens arising from BVDV in collected sera. A total of 240 individual faecal samples from four age groups were examined for the presence of E. bieneusi by nested PCR. Sequence analysis of six E. bieneusi positive samples revealed the presence of the genotype I of E. bieneusi, previously described in cattle. The hypothesis expecting higher prevalence of E. bieneusi in BVDV positive cattle herds was not confirmed in this study; however this is the first description about E. bieneusi in cattle in the Czech Republic.
- MeSH
- Bovine Virus Diarrhea-Mucosal Disease complications microbiology virology MeSH
- Enzyme-Linked Immunosorbent Assay veterinary MeSH
- Enterocytozoon * genetics MeSH
- Feces microbiology MeSH
- Genotype MeSH
- Microsporidiosis epidemiology etiology microbiology veterinary virology MeSH
- Cattle Diseases epidemiology microbiology MeSH
- Polymerase Chain Reaction veterinary MeSH
- Prevalence MeSH
- Sequence Analysis, DNA veterinary MeSH
- Cattle parasitology virology MeSH
- Age Factors MeSH
- Diarrhea Viruses, Bovine Viral * MeSH
- Animals MeSH
- Check Tag
- Cattle parasitology virology MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- MeSH
- Antigens, Viral genetics MeSH
- Respiratory Syncytial Virus, Bovine genetics MeSH
- Research Support as Topic MeSH
- Phylogeny MeSH
- Genetic Variation MeSH
- Molecular Sequence Data MeSH
- Antibodies, Monoclonal MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Antibodies, Viral MeSH
- Restriction Mapping methods MeSH
- Amino Acid Sequence MeSH
- Sequence Homology, Amino Acid MeSH
- Sequence Alignment MeSH
- Viral Proteins genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- MeSH
- Respiratory Syncytial Virus, Bovine genetics immunology classification MeSH
- Research Support as Topic MeSH
- Phylogeny MeSH
- Respiratory Syncytial Virus Infections epidemiology veterinary virology MeSH
- Molecular Sequence Data MeSH
- Cattle Diseases epidemiology virology MeSH
- Viral Envelope Proteins MeSH
- Amino Acid Sequence MeSH
- Cattle MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
