Fecal microbiota transfer may serve as a therapeutic tool for treating obesity and related disorders but currently, there is no consensus regarding the optimal donor characteristics. We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects. Ex-germ-free animals were colonized with human vegan microbiota and fed a standard or Western-type diet (WD) with or without inulin supplementation. Despite the colonization with vegan microbiota, WD induced excessive weight gain, impaired glucose metabolism, insulin resistance, and liver steatosis. However, supplementation with inulin reversed steatosis and improved glucose homeostasis. In contrast, inulin did not affect WD-induced metabolic changes in non-humanized conventional mice. In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA). We found that (i) vegan microbiota alone does not protect against adverse effects of WD; and (ii) supplementation with inulin reversed steatosis and normalized glucose metabolism. This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.
- MeSH
- fekální transplantace MeSH
- glukosa farmakologie MeSH
- inulin farmakologie MeSH
- lidé MeSH
- myši MeSH
- potravní vláknina farmakologie MeSH
- střevní mikroflóra * MeSH
- vegani MeSH
- západní dieta MeSH
- ztučnělá játra * prevence a kontrola farmakoterapie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
V Európskej únii zažije nejakú formu psychickej poruchy aspoň raz za život približne jeden zo siedmich ľudí. Napriek tomu, že prevalencia psychických porúch je vysoká a narastá aj počet liečených, stále zostáva veľký počet tých, ktorí nevyhľadajú odbornú pomoc. Existujú reálne obavy, že duševné poruchy, ktoré sú aj veľmi ekonomicky nákladné, budú do roku 2030 hlavnou príčinou zdravotného postihnutia vo vyspelých krajinách. Pritom priame náklady na zdravotnú starostlivosť sú obvykle oveľa nižšie ako náklady na nepriamu, sociálnu starostlivosť. Správne nastavenie systému starostlivosti o duševné zdravie a alokáciu zodpovedajúcich finančných zdrojov limituje nedostatok epidemiologických údajov. Na Slovensku sú k dispozícii len epidemiologické údaje o prevalencii depresie, úzkostných porúch, alkoholizmu a fajčenia z výskumov ešte zo začiatku 21. storočia, ale chýbajú novšie údaje, resp. dáta o prevalencii iných duševných porúch. Na celkový manažment psychických porúch a ich sociálny dopad má významný vplyv ich včasná diagnostika a na dôkazoch založená liečba. V tejto oblasti nás čakajú zmeny v podobe 11. revízie Medzinárodnej klasifikácie chorôb (MKCH-11) a dôslednej implementácie schválených Štandardných diagnostických a terapeutických postupov. Podľa údajov Národného centra zdravotníckych informácií boli v roku 2018 najčastejšími diagnostickými kategóriami v starostlivosti psychiatrických ambulancií na Slovensku afektívne poruchy, neurotické, stresom podmienené a somatoformné poruchy a organické duševné choroby vrátane symptomatických, z ktorých najväčší podiel tvorili demencie. V práci analyzujeme tieto tri diagnostické okruhy z hľadiska ich postavenia v klasifikácii MKCH-11, podľa ich výskytu a ich dopadu na celkové duševné a telesné zdravie a funkčnosť pacientov a tiež ich ekonomickej náročnosti pre spoločnosť. Tieto poznatky sú podkladom pre správne nastavenie jednotlivých krokov reformy psychiatrickej starostlivosti, ktorá na Slovensku prebieha.
In the European Union every seventh person experiences any form of mental disorder at least one time during the life. Despite the fact, that the prevalence of mental disorders is high, and the number of treated patients is rising, still remains a large number of those, who never find out any professional help. Actual concern exists, that mental disorders associated with high economic burden, will be the main reason ofdisability in developed countries by the year 2030. And besides, direct costs of the health care are usually much lower than indirect costs for the social care. Accurate adjustment of the mental health care system and allocation of the appropriate financial sources is limited by missing epidemiological data. In Slovakia are available only data about the prevalence of depression, anxiety disorders, alcoholism and smoking from the research at the beginning of 21st century, but the newer data or the data about other mental disorders are missing. Global management of mental disorders and their social impact is very much influenced by early diagnostics and evidence-based treatment. In this field we are expecting some changes in 11th revision of International Classification of the Diseases (ICD-11) and rigorous implementation of approved Standard Diagnostic and Therapeutic Procedures. According to the data of National Health Information Center the most frequent diagnostic categories at the psychiatric outpatient care in Slovakia in 2018 were affective disorders, neurotic, stress and somatoform disorders and organic mental disorders including symptomatic disorders, with the greatest contribution of dementias. In this paper we analyse these three diagnostic categories in terms of their position in the ICD-11, their prevalence in the different population groups, their impact on overall mental and physical health and the functioning of the patient and also their economical burden for the society. This information represents the base for accurate setting-up of individual steps of the reform of psychiatric care, which takes place in Slovakia.
- MeSH
- ambulantní péče MeSH
- demence diagnóza epidemiologie klasifikace MeSH
- depresivní poruchy diagnóza epidemiologie klasifikace MeSH
- duševně nemocní MeSH
- lidé MeSH
- mezinárodní klasifikace nemocí * trendy MeSH
- prevalence MeSH
- služby péče o duševní zdraví MeSH
- úzkostné poruchy diagnóza epidemiologie klasifikace MeSH
- Check Tag
- lidé MeSH
- Geografické názvy
- Slovenská republika MeSH
Background and Aim: Plant-based diets are associated with potential health benefits, but the contribution of gut microbiota remains to be clarified. We aimed to identify differences in key features of microbiome composition and function with relevance to metabolic health in individuals adhering to a vegan vs. omnivore diet. Methods: This cross-sectional study involved lean, healthy vegans (n = 62) and omnivore (n = 33) subjects. We assessed their glucose and lipid metabolism and employed an integrated multi-omics approach (16S rRNA sequencing, metabolomics profiling) to compare dietary intake, metabolic health, gut microbiome, and fecal, serum, and urine metabolomes. Results: The vegans had more favorable glucose and lipid homeostasis profiles than the omnivores. Long-term reported adherence to a vegan diet affected only 14.8% of all detected bacterial genera in fecal microbiome. However, significant differences in vegan and omnivore metabolomes were observed. In feces, 43.3% of all identified metabolites were significantly different between the vegans and omnivores, such as amino acid fermentation products p-cresol, scatole, indole, methional (lower in the vegans), and polysaccharide fermentation product short- and medium-chain fatty acids (SCFAs, MCFAs), and their derivatives (higher in the vegans). Vegan serum metabolome differed markedly from the omnivores (55.8% of all metabolites), especially in amino acid composition, such as low BCAAs, high SCFAs (formic-, acetic-, propionic-, butyric acids), and dimethylsulfone, the latter two being potential host microbiome co-metabolites. Using a machine-learning approach, we tested the discriminative power of each dataset. Best results were obtained for serum metabolome (accuracy rate 91.6%). Conclusion: While only small differences in the gut microbiota were found between the groups, their metabolic activity differed substantially. In particular, we observed a significantly different abundance of fermentation products associated with protein and carbohydrate intakes in the vegans. Vegans had significantly lower abundances of potentially harmful (such as p-cresol, lithocholic acid, BCAAs, aromatic compounds, etc.) and higher occurrence of potentially beneficial metabolites (SCFAs and their derivatives).
- Publikační typ
- časopisecké články MeSH
Meduca (Solen)
Druhé prepracované vydanie 406 stran : ilustrace ; 25 cm
Publikácia sa zameriava na anatómiu, histológiu a fyziológiu nervového systému. Určené odbornej verejnosti.
- MeSH
- histologie MeSH
- nervový systém - fyziologické jevy MeSH
- nervový systém anatomie a histologie MeSH
- neuroanatomie MeSH
- neurofyziologie MeSH
- Publikační typ
- monografie MeSH
- Konspekt
- Anatomie člověka a srovnávací anatomie
- NLK Obory
- neurovědy
