Saliva contains possible biomarkers that are associated with dental caries. The present study aimed to analyse differences in the abundance of proteins in the saliva between caries-positive (CP; N = 15) and caries-free (CF; N = 12) males and to compare differences in the abundance of proteins between two saliva sample fractions (supernatant and pellet). We found 14 differently significantly expressed proteins in the CF group when comparing the supernatant fractions of the CP and CF groups, and three proteins in the pellet fractions had significantly higher expression in the CP group. Our results indicate very specific protein compositions of the saliva in relation to dental caries resistance (the saliva of the CP group mainly contained pellet proteins and the saliva of the CF group mainly contained supernatant proteins). This was the first time that the saliva pellet fraction was analysed in relation to the dental caries status. We detected specific calcium-binding proteins that could have decalcified enamel in the saliva pellet of the CP group. We also observed significantly up-regulated immune proteins in the saliva supernatant of the CF group that could play an important role in the caries prevention. The particular protein compositions of the saliva pellet and supernatant in the groups with different susceptibilities to tooth decay is a promising finding for future research.

• MeSH
• lidé MeSH
• náchylnost k zubnímu kazu * MeSH
• proteomika * MeSH
• slinné proteiny a peptidy analýza   imunologie   metabolismus   MeSH
• sliny chemie   imunologie   metabolismus   MeSH
• zubní kaz imunologie   metabolismus   prevence a kontrola   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Background: The rumen microbiota is one of the most complex consortia of anaerobes, involving archaea, bacteria, protozoa, fungi and phages. They are very effective at utilizing plant polysaccharides, especially cellulose and hemicelluloses. The most important hemicellulose decomposers are clustered with the genus Butyrivibrio. As the related species differ in their range of hydrolytic activities and substrate preferences, Butyrivibrio fibrisolvens was selected as one of the most effective isolates and thus suitable for proteomic studies on substrate comparisons in the extracellular fraction. The B. fibrisolvens genome is the biggest in the butyrivibria cluster and is focused on "environmental information processing" and "carbohydrate metabolism". Methods: The study of the effect of carbon source on B. fibrisolvens 3071 was based on cultures grown on four substrates: xylose, glucose, xylan, xylan with 25% glucose. The enzymatic activities were studied by spectrophotometric and zymogram methods. Proteomic study was based on genomics, 2D electrophoresis and nLC/MS (Bruker Daltonics) analysis. Results: Extracellular β-endoxylanase as well as xylan β-xylosidase activities were induced with xylan. The presence of the xylan polymer induced hemicellulolytic enzymes and increased the protein fraction in the interval from 40 to 80 kDa. 2D electrophoresis with nLC/MS analysis of extracellular B. fibrisolvens 3071 proteins found 14 diverse proteins with significantly different expression on the tested substrates. Conclusion: The comparison of four carbon sources resulted in the main significant changes in B. fibrisolvens proteome occurring outside the fibrolytic cluster of proteins. The affected proteins mainly belonged to the glycolysis and protein synthesis cluster.

• Publikační typ
• časopisecké články MeSH

Idiopathic pes equinovarus (clubfoot) is a congenital deformity of the feet and lower legs. Clubfoot belongs to a group of fibro-proliferative disorders but its origin remains unknown. Our study aimed to achieve the first complex proteomic comparison of clubfoot contracted tissue of the foot (medial side; n = 16), with non-contracted tissue (lateral side; n = 13). We used label-free mass spectrometry quantification and immunohistochemistry. Seven proteins were observed to be significantly upregulated in the medial side (asporin, collagen type III, V, and VI, versican, tenascin-C, and transforming growth factor beta induced protein) and four in the lateral side (collagen types XII and XIV, fibromodulin, and cartilage intermediate layer protein 2) of the clubfoot. Comparison of control samples from cadavers brought only two different protein concentrations (collagen types I and VI). We also revealed pathological calcification and intracellular positivity of transforming growth factor beta only in the contracted tissue of clubfoot. Most of the 11 differently expressed proteins are strongly related to the extracellular matrix architecture and we assume that they may play specific roles in the pathogenesis of this deformity. These proteins seem to be promising targets for future investigations and treatment of this disease. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.

• MeSH
• dítě MeSH
• extracelulární matrix - proteiny metabolismus   MeSH
• hmotnostní spektrometrie MeSH
• kalcinóza MeSH
• lidé MeSH
• pes equinovarus etiologie   metabolismus   MeSH
• předškolní dítě MeSH
• prospektivní studie MeSH
• proteom MeSH
• transformující růstový faktor beta metabolismus   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

Most people worldwide suffer from dental caries. Only a small part of the population is cariesresistant and the reason for this resistance in unknown. Only a few studies compared the saliva protein composition of persons with carious teeth and persons with no caries. Our study is the first to relate proteomic analysis of the caries aetiology with gender. In this study, we compared the differences in the abundances of proteins in the saliva between cariesresistant and caries-susceptible females and males by nano-liquid chromatography-tandem mass spectrometry (Label-Free Quantitative Proteomics). Our results demonstrate that the observed differences in the protein levels might have an influence on anticaries resistance. A total of 19 potential markers of tooth caries were found, for example proteins S100A8 and annexin A1 with higher expression in the cariessusceptible group in comparison with the caries-free group and mucin-5B, lactoferrin, lysozyme C with higher expression in the caries-free group in comparison with the caries-susceptible group. The presented study is the first complex proteomic and gender project where the saliva protein content of caries-free and caries-susceptible persons were compared by label-free MS. The newly detected potential protein markers of dental caries can be a good basis for further research and for possible future therapeutic use.

• MeSH
• chromatografie kapalinová MeSH
• dospělí MeSH
• hmotnostní spektrometrie MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• náchylnost k zubnímu kazu * MeSH
• slinné proteiny a peptidy * analýza   MeSH
• sliny chemie   MeSH
• zubní kaz MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH

The heart is characterized by a remarkable degree of heterogeneity. Since different cardiac pathologies affect different cardiac regions, it is important to understand molecular mechanisms by which these parts respond to pathological stimuli. In addition to already described left ventricular (LV)/right ventricular (RV) and transmural differences, possible baso-apical heterogeneity has to be taken into consideration. The aim of our study has been, therefore, to compare proteomes in the apical and basal parts of the rat RV and LV. Two-dimensional electrophoresis was used for the proteomic analysis. The major result of this study has revealed for the first time significant baso-apical differences in concentration of several proteins, both in the LV and RV. As far as the LV is concerned, five proteins had higher concentration in the apical compared to basal part of the ventricle. Three of them are mitochondrial and belong to the "metabolism and energy pathways" (myofibrillar creatine kinase M-type, L-lactate dehydrogenase, dihydrolipoamide dehydrogenase). Myosin light chain 3 is a contractile protein and HSP60 belongs to heat shock proteins. In the RV, higher concentration in the apical part was observed in two mitochondrial proteins (creatine kinase S-type and proton pumping NADH:ubiquinone oxidoreductase). The described changes were more pronounced in the LV, which is subjected to higher workload. However, in both chambers was the concentration of proteins markedly higher in the apical than that in basal part, which corresponds to the higher energetic demand and contractile activity of these segments of both ventricles.

• MeSH
• 2D gelová elektroforéza MeSH
• chaperon hsp60 metabolismus   MeSH
• chromatografie kapalinová MeSH
• dihydrolipoamiddehydrogenasa metabolismus   MeSH
• energetický metabolismus MeSH
• kreatinkinasa, forma MM metabolismus   MeSH
• L-laktátdehydrogenasa metabolismus   MeSH
• lehké řetězce myosinu metabolismus   MeSH
• mitochondriální proteiny metabolismus   MeSH
• potkani Wistar MeSH
• proteomika * MeSH
• respirační komplex I metabolismus   MeSH
• srdeční komory enzymologie   metabolismus   MeSH
• svalové proteiny izolace a purifikace   metabolismus   MeSH
• tandemová hmotnostní spektrometrie MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Isolated porcine pepsinogen A was used for the preparation of polyclonal rabbit and polyclonal chicken anti-pepsinogen A antibodies. Immunochemical properties of both immunoglobulin fractions were compared. The rabbit anti-serum was further purified using immobilized porcine pepsinogen A on magnetic cellulose beads and the resulting anti-pepsinogen A fraction proved to be applicable for the separation and the determination of porcine pepsinogen A. In contrary, antibodies prepared from chicken eggs by the same way have been found not suitable for the evaluation of the pepsinogen A level. Unexpectedly, the pre-immune fraction of chicken antibodies showed reactivity against porcine pepsinogen A and the affinity separation of specific polyclonal chicken anti-pepsinogen A antibodies on immobilized porcine pepsinogen A did not result in an enrichment of anti-pepsinogen A antibodies.

• MeSH
• ELISA MeSH
• imunohistochemie MeSH
• králíci MeSH
• kur domácí MeSH
• nádory žaludku imunologie   metabolismus   MeSH
• pepsinogen A imunologie   metabolismus   MeSH
• prasata MeSH
• protilátky imunologie   MeSH
• skot MeSH
• žaludeční sliznice imunologie   metabolismus   patologie   MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH