Heat-inactivated Lactobacillus casei LOCK 0900, L. casei LOCK 0908 and Lactobacillus paracasei LOCK 0919 strains, applied to blood cell cultures obtained from children with atopic dermatitis induced production of anti-allergic T^sub H^1 cytokines (interleukin-12, interleukin-18, interferon-?, tumor necrosis factor-?) and regulatory transforming growth factor-ß^sub 1^), but did not stimulate pro-allergic interleukin-5. The lactobacilli-mixture remarkably enhanced the T^sub H^1 response compared to single strains. This synergistic effect was not observed for transforming growth factor-ß^sub 1^. In contrast, the amount of interleukin-10 was found to be considerably lower when cells were stimulated with lactobacilli-mixture compared to single strains. The mixture of Lactobacillus strains represents a probiotic bacterial preparation modulating in vitro cytokine profile of allergic children towards anti-allergic T^sub H^1 response

• MeSH
• atopická dermatitida imunologie   MeSH
• cytokiny sekrece   MeSH
• financování organizované MeSH
• imunologické faktory farmakologie   MeSH
• kojenec MeSH
• kultivované buňky MeSH
• Lactobacillus imunologie   MeSH
• leukocyty mononukleární imunologie   MeSH
• lidé MeSH
• probiotika farmakologie   MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH

Three Lactobacillus strains (LOCK 0900, LOCK 0908, LOCK 0919) out of twenty-four isolates were selected according to their antagonistic activity against pathogenic bacteria, resistance to low pH and milieu of bile salts. Intragastric administration of a mixture of these strains to Balb/c mice affected cytokine T(H)1-T(H)2 balance toward nonallergic T(H)1 response. Spleen cells, isolated from lactobacilli-treated mice and re-stimulated in vitro with the mixture of heat-inactivated tested strains, produced significantly higher amounts of anti-allergic tumor necrosis factor- and interferon-gamma than control animals whereas the level of pro-allergic interleukin-5 was significantly lower. Lactobacillus cells did not translocate through the intestinal barrier into blood, liver and spleen; a few Lactobacillus cells found in mesenteric lymph nodes could create antigenic reservoir activating the immune system. The mixture of Lactobacillus LOCK 0900, LOCK 0908 and LOCK 0919 strains represents a probiotic bacterial preparation with possible use in prophylaxis and/or therapy of allergic diseases.

• MeSH
• antibakteriální látky farmakologie   MeSH
• antibióza MeSH
• bakteriální translokace MeSH
• financování organizované MeSH
• fyziologický stres MeSH
• interferon gama sekrece   MeSH
• interleukin-5 sekrece   MeSH
• játra mikrobiologie   MeSH
• krev mikrobiologie   MeSH
• kultivované buňky MeSH
• kyseliny farmakologie   MeSH
• Lactobacillus fyziologie   účinky léků   MeSH
• leukocyty mononukleární imunologie   MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• probiotika aplikace a dávkování   farmakologie   MeSH
• slezina imunologie   mikrobiologie   MeSH
• TNF-alfa sekrece   MeSH
• žlučové kyseliny a soli farmakologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH