Susceptibility in vitro and trends in resistance to antimicrobials were determined by a dilution micromethod in a group of Actinobacillus pleuropneumoniae, Pasteurella multocida, Mannheimia haemolytica and Escherichia coli isolates from clinical cases of cattle and swine diseases in the Czech Republic from 2007 to 2011. A high susceptibility of pig and cattle respiratory pathogens to antimicrobials was found, with the exception of the moderate prevalence of M. haemolytica resistance to ampicillin. In contrast to respiratory pathogens, low susceptibility of E. coli of pig and cattle isolates to ampicillin and amoxicillin/clavulanic acid was noted. Regarding resistance trends, an increase in levels of resistance among E. coli isolates to ampicillin and amoxicillin/clavulanic acid was identified, but the resistance of respiratory isolates was low, with the exception of M. haemolytica. For the period of 2007-2011, there was a significant and almost continuous increase in sales (compared with population correction unit) of ceftiofur, cefquinome and other beta lactams for pigs. Consumption peaked in 2010. In the case of amoxicillin in combination with clavulanic acid, data showed a significant decrease in sales from 2007 to 2008, followed by a period of fluctuation. In cattle, within the groups of 3rd and 4th generation cephalosporins and for the whole group of other betalactams for the period of 2007-2011, there was a significant and almost continuous increase in sales (compared with population correction unit). Consumption peaked in 2010. In the case of ceftiofur, there was a huge increase noted from 2010. In the case of amoxicillin in combination with betalactamase inhibitor (clavulanic acid) data shows a significant decrease from 2007 to 2008, followed by a period of fluctuation in sales.
- MeSH
- Actinobacillus pleuropneumoniae účinky léků MeSH
- bakteriální léková rezistence genetika MeSH
- beta-laktamy metabolismus MeSH
- Escherichia coli účinky léků MeSH
- infekce bakteriemi čeledi Pasteurellaceae epidemiologie veterinární MeSH
- infekce vyvolané Escherichia coli epidemiologie veterinární MeSH
- inhibitory beta-laktamasy farmakologie MeSH
- Mannheimia haemolytica účinky léků MeSH
- mikrobiální testy citlivosti veterinární MeSH
- nemoci prasat epidemiologie mikrobiologie MeSH
- nemoci skotu epidemiologie mikrobiologie MeSH
- Pasteurella multocida účinky léků MeSH
- prasata MeSH
- prevalence MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
The protective role of hyperimmune serum in the prevention of Haemophilus parasuis infections in post-weaned piglets was assessed by experimental challenge. The hyperimmune serum was obtained from a pig vaccinated with a commercial vaccine against Glässer's disease. Thirty-eight weaned piglets were divided into four groups: three groups were immunised intramuscularly with 10 ml of hyperimmune serum and one group consisted of unimmunised control animals. All piglets were subsequently infected intraperitoneally with H. parasuis serotype 5 at different times after immunisation. The use of hyperimmune serum provided the piglets with partial protection against experimental infection. The levels of protection indirectly depend on time between serum inoculation and challenge infection. The best protection of piglets against experimental infection was obtained in the group immunised 1 week before inoculation; the same group in which the highest levels of antibodies were detected at the time of challenge.
- MeSH
- ELISA veterinární MeSH
- Haemophilus parasuis imunologie izolace a purifikace MeSH
- hemofilové infekce imunologie prevence a kontrola veterinární MeSH
- hemofilové vakcíny imunologie MeSH
- lidé MeSH
- nemoci prasat imunologie prevence a kontrola MeSH
- odstavení MeSH
- pasivní imunizace metody MeSH
- polymerázová řetězová reakce veterinární MeSH
- prasata MeSH
- protilátky bakteriální krev MeSH
- Sus scrofa MeSH
- tkáňová distribuce MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
