In contrast to other retroviruses, Mason-Pfizer monkey virus (M-PMV) assembles immature capsids in the cytoplasm. We have compared the ability of minimal assembly-competent domains from M-PMV and human immunodeficiency virus type 1 (HIV-1) to assemble in vitro into virus-like particles in the presence and absence of nucleic acids. A fusion protein comprised of the capsid and nucleocapsid domains of Gag (CANC) and its N-terminally modified mutant (DeltaProCANC) were used to mimic the assembly of the viral core and immature particles, respectively. In contrast to HIV-1, where CANC assembled efficiently into cylindrical structures, the same domains of M-PMV were assembly incompetent. The addition of RNA or oligonucleotides did not complement this defect. In contrast, the M-PMV DeltaProCANC molecule was able to assemble into spherical particles, while that of HIV-1 formed both spheres and cylinders. For M-PMV, the addition of purified RNA increased the efficiency with which DeltaProCANC formed spherical particles both in terms of the overall amount and the numbers of completed spheres. The amount of RNA incorporated was determined, and for both rRNA and MS2-RNA, quantities similar to that of genomic RNA were encapsidated. Oligonucleotides also stimulated assembly; however, they were incorporated into DeltaProCANC spherical particles in trace amounts that could not serve as a stoichiometric structural component for assembly. Thus, oligonucleotides may, through a transient interaction, induce conformational changes that facilitate assembly, while longer RNAs appear to facilitate the complete assembly of spherical particles.
- MeSH
- bezbuněčný systém MeSH
- financování organizované MeSH
- genom virový fyziologie MeSH
- genové produkty gag genetika metabolismus MeSH
- HIV-1 fyziologie izolace a purifikace ultrastruktura MeSH
- kapsida metabolismus ultrastruktura MeSH
- konformace proteinů MeSH
- lidé MeSH
- Masonův-Pfizerův opičí virus fyziologie izolace a purifikace MeSH
- mutace MeSH
- oligonukleotidy genetika metabolismus MeSH
- RNA ribozomální genetika metabolismus MeSH
- RNA virová genetika metabolismus ultrastruktura MeSH
- sestavení viru fyziologie MeSH
- terciární struktura proteinů účinky léků MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- dějiny 20. století MeSH
- mikrobiologie * dějiny MeSH
- Check Tag
- dějiny 20. století MeSH
- Publikační typ
- biografie MeSH
- osobní vyprávění MeSH
- O autorovi
- Rýc, Miloš, 1938-1989 Autorita
352 s.
- Konspekt
- Všeobecná příručková díla
- NLK Obory
- všeobecná příručková díla a encyklopedie
- NLK Publikační typ
- učebnice vysokých škol
2. část. přeprac. vyd. 82 s. : il.
- Konspekt
- Biologické vědy
- NLK Obory
- biologie
- zdravotní výchova
- NLK Publikační typ
- učebnice vysokých škol
