Hodnocení polymorfismů a jejich vzájemných vztahů u genů DNA reparace a u genů účastnících se biotransformace u pacientů s nádorovým onemocněním a u zdravých kontrol v české populaci. Porovnání profilů expresí XME a DNA reparačních genů s genetickými profily a konfrontace s výsledky funkčních testů. Zavedení metod pro stanovení jednonukleotidového polymorfismu (SNP) v různých DNA reparačních genech (XPA, hOGG1, APE1 a NBS1). DNA bude izolována z leukocytů a XME i DNA reparační genetické polymorfismy budou stanovovány metodou PCR-RFLP.; Evaluation of links between individual genetic background - evaluation of polymorphisms in both DNA repair and XME genes and expression levels in lymphocytes in cancer patients and healthy controls in Czech Republic. Determination of expression profilesof DNA repair and XME genes in comparison with genetic profiles and outcomes from the functional tests. A study on genotype-phenotype relations with special regard to prognosis and therapy outcome.

• MeSH
• Biotransformation MeSH
• Genetic Predisposition to Disease MeSH
• Genetic Testing MeSH
• Disease Susceptibility MeSH
• Neoplastic Stem Cells physiology   MeSH
• Polymorphism, Genetic MeSH
• Recombination, Genetic MeSH

• Conspectus
• Biochemie. Molekulární biologie. Biofyzika
• NML Fields
• biologie
• farmacie a farmakologie
• onkologie
• NML Publication type
• závěrečné zprávy o řešení grantu IGA MZ ČR

New urinary adenine adducts, 3-(2-hydroxy-1-phenylethyl)adenine (N3alphaA), 3-(2-hydroxy-2-phenylethyl)adenine (N3betaA), were found in the urine of mice exposed to styrene vapour. These styrene 7,8-oxide derived adenine adducts as well as previously identified guanine adducts, 7-(2-hydroxy-1-phenylethyl)guanine (N7alphaG) and 7-(2-hydroxy-2-phenylethyl)guanine (N7betaG) were quantified by HPLC-ESI-MS(2) and the excretion profile during and after a repeated exposure to 600mg/m(3) or 1200mg/m(3) of styrene for 10 consecutive days (6h/day) was determined. The excretion was dose dependent. Total N3 adenine adducts (N3alphaA+N3betaA) excreted amounted to nearly 0.8x10(-5)% of the absorbed dose while urinary N7 guanine adducts (N7alphaG+N7betaG) amounted to nearly 1.4x10(-5)% of the dose. No accumulation of the adducts was observed. Due to rapid depurination from the DNA, the excretion of both N3 adenine and N7 guanine adducts ceased shortly after finishing the exposure. Both N3 adenine and N7 guanine adducts may be used as non-invasive biomarkers of effective dose reflecting only a short time exposure to styrene.

• MeSH
• Adenine analogs & derivatives   urine   MeSH
• DNA Adducts analysis   urine   MeSH
• Administration, Inhalation MeSH
• Financing, Organized MeSH
• Guanine analogs & derivatives   urine   MeSH
• Mice MeSH
• Styrene administration & dosage   metabolism   MeSH
• Chromatography, High Pressure Liquid MeSH
• Animals MeSH
• Check Tag
• Male MeSH
• Mice MeSH
• Animals MeSH