In many eukaryotes, a significant part of the plasma membrane is closely associated with the dynamic meshwork of cortical endoplasmic reticulum (cortical ER). We mapped temporal variations in the local coverage of the yeast plasma membrane with cortical ER pattern and identified micron-sized plasma membrane domains clearly different in cortical ER persistence. We show that clathrin-mediated endocytosis is initiated outside the cortical ER-covered plasma membrane zones. These cortical ER-covered zones are highly dynamic but do not overlap with the immobile and also endocytosis-inactive membrane compartment of Can1 (MCC) and the subjacent eisosomes. The eisosomal component Pil1 is shown to regulate the distribution of cortical ER and thus the accessibility of the plasma membrane for endocytosis.
- MeSH
- buněčná membrána fyziologie MeSH
- endocytóza MeSH
- endoplazmatické retikulum fyziologie MeSH
- fosfoproteiny fyziologie MeSH
- klathrin fyziologie MeSH
- Saccharomyces cerevisiae - proteiny fyziologie MeSH
- Saccharomyces cerevisiae fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- lidé MeSH
- rozpustnost MeSH
- Saccharomyces cerevisiae fyziologie MeSH
- transport proteinů MeSH
- Check Tag
- lidé MeSH
- MeSH
- biologický transport MeSH
- buněčná membrána fyziologie MeSH
- Publikační typ
- kongresy MeSH
