This review provides a brief survey of the biological effects of selected endocrine-disrupting compounds that are formed after internal exposure of organisms. Further, the present analytical methods available for the determination of these compounds in foodstuffs are critically evaluated. The attention is primarily devoted to the methods for sample pretreatment, which are the main source of errors and are usually the most time-consuming step of the whole analysis. This review is focused on selected natural and synthetic estrogens, estrogen conjugates, and chemical additives used in the plastic industry that can act as estrogen mimics.
- MeSH
- analýza potravin * MeSH
- endokrinní disruptory analýza MeSH
- estrogeny analýza MeSH
- lidé MeSH
- plastické hmoty chemie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
A review of the current knowledge of the styrene and styrene oxide metabolism in laboratory animals and humans. Styrene ranks among the most important monomers in the manufacture of plastics and styrene oxide is the main intermediate involved in its metabolism. Both chemicals exhibit adverse effects. Various analytical methods have been developed for assessing their concentrations in organisms. Determination of their protein adducts shows several advantages over their determination in urine or as DNA adducts due to their stability and easy availability. The protein adduct determination by a modified Edman degradation, Raney-nickel cleavage, alkaline hydrolysis and enzymatic hydrolysis is described. Styrene oxide adducts with various globin amino acids have been also studied by these methods. A modified Edman degradation has proved to be a most sensitive method, with a limit of detection of the order of pmol per g of globin.
Interactions of boar, bull, and human seminal plasma proteins with heparin and phosphorylcholine were studied by affinity LC using heparin immobilized to a Toyopearl support. A step gradient elution from 0.15 to 1.50 M NaCl was employed to elute the seminal plasma proteins. Relative amounts of the heparin-binding fraction of seminal plasma proteins (H+) in seminal plasma of three species were determined. Further on, the fraction of seminal plasma proteins interacting with phosphorylcholine-binding proteins (P+) was evaluated. P+ proteins were not found in human seminal plasma and their highest amount was present in bull seminal plasma. A CE method was developed for separation of seminal plasma proteins. Various capillaries and separation conditions were tested; the best resolution was obtained in a bare-silica capillary, with a micellar system consisting of a 0.02 M borate buffer and 0.05 M SDS pH 10.0. The optimized conditions were applied to the identification of the components in boar plasma.
- MeSH
- chromatografie afinitní metody MeSH
- chromatografie kapalinová metody MeSH
- elektroforéza kapilární metody MeSH
- financování organizované MeSH
- fosforylcholin chemie MeSH
- heparin chemie MeSH
- lidé MeSH
- protein - isoformy analýza MeSH
- proteiny semenné plazmy analýza MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- hodnotící studie MeSH
- MeSH
- chemické techniky analytické metody normy MeSH
- chemie fyzikální metody normy MeSH
- chromatografie na tenké vrstvě metody využití MeSH
- chromatografie plynová metody využití MeSH
- elektroforéza kapilární metody využití MeSH
- extrakce na pevné fázi metody využití MeSH
- herbicidy analýza chemie toxicita MeSH
- klinické laboratorní techniky využití MeSH
- pesticidy analýza chemie toxicita MeSH
- vysokoúčinná kapalinová chromatografie metody trendy využití MeSH
- MeSH
- chemické techniky analytické metody normy MeSH
- chemie fyzikální metody normy MeSH
- chromatografie afinitní metody přístrojové vybavení využití MeSH
- chromatografie kapalinová metody využití MeSH
- klinické laboratorní techniky využití MeSH
- ligandy MeSH
- organické látky chemie izolace a purifikace normy MeSH
- pufry MeSH
Three stationary phases have been prepared for affinity liquid chromatography isolation and separation of porcine and human pepsin. The phases contain 3,5-diiodo-L-tyrosine (DIT) bound to the supports HEMA BIO VS, HEMA BIO E and EPOXY TOYOPEARL. These phases have been tested on a model sample of porcine pepsin A and applied to human pepsin. Fractions have been collected and the chymase activity determined in selected analyses. For affinity CE, capillaries have been prepared by modifying the wall with 3-aminopropyltriethoxysilane, followed either by direct binding of DIT, or by binding L-tyrosine that was subsequently iodated. The dissociation constant K(d) has been determined for the pepsin-DIT complex from the changes in the electrophoretic mobilities.
- MeSH
- chromatografie afinitní metody přístrojové vybavení MeSH
- dijodtyrosin chemie MeSH
- elektroforéza kapilární metody přístrojové vybavení MeSH
- finanční podpora výzkumu jako téma MeSH
- lidé MeSH
- oxid křemičitý chemie MeSH
- pepsin A izolace a purifikace MeSH
- prasata MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
