OBJECTIVES: Intracoronary cell transplantation during catheter balloon inflations may be associated with adverse events. We studied the effectiveness of an alternative transplantation technique--intracoronary cell infusion. METHODS: Fourteen pigs, which had survived acute myocardial infarction, were randomized into 2 treatment groups and 2 controls. Three days after infarction, 12 pigs underwent allogeneic intracoronary mononuclear bone marrow cell transplantation using either the standard technique (short-term cell injections during repeat balloon inflations, technique A, n = 6) or continuous intracoronary cell infusion without balloon inflations (technique B, n = 6). Implanted cells were stained with fluorescent dye. After transplantation, the pigs were euthanized and myocardial samples were analyzed by fluorescent microscopy. RESULTS: The mean numbers of fluorescently labeled bone marrow cells in the infarction border zone, in the infarction mid-area and in the center of myocardial infarction were 84, 72 and 55 using technique A, and 29, 57 and 46 using technique B, respectively. The mean cell retention in the infarction border zone of 84 cells for technique A and 29 cells for technique B differed significantly (p = 0.034, two-tailed t test). CONCLUSION: The continuous intracoronary cell infusion technique is a less efficient cell delivery technique as compared with the standard technique using repeat intracoronary balloon inflations.
- MeSH
- financování organizované MeSH
- fluoresceiny MeSH
- fluorescenční barviva MeSH
- fluorescenční mikroskopie MeSH
- infarkt myokardu terapie MeSH
- katetrizace MeSH
- modely nemocí na zvířatech MeSH
- prasata MeSH
- srdeční katetrizace MeSH
- transplantace kostní dřeně metody MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- MeSH
- finanční podpora výzkumu jako téma MeSH
- hematopoetické kmenové buňky cytologie MeSH
- infarkt myokardu patofyziologie veterinární MeSH
- králíci anatomie a histologie MeSH
- modely nemocí na zvířatech MeSH
- železo diagnostické užití MeSH
- zvířata MeSH
- Check Tag
- králíci anatomie a histologie MeSH
- zvířata MeSH
